Regulation of CpG-induced immune activation by suppressive oligodeoxynucleotides.

Date

2003-12-01

Author

Klinman, DM
Zeuner, R
Yamada, H
Gürsel, Mayda
Currie, D
Gursel, I

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

195
views

0
downloads

Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated "CpG motifs" stimulate an innate immune response characterized by the production of cytokines, chemokines, and polyreactive Igs that promote host survival following infectious challenge. Yet CpG-driven immune activation can have deleterious consequences, such as increasing the host's susceptibility to autoimmune disease. The immunomodulatory activity of CpG DNA can be blocked by DNA containing "suppressive" motifs. This work explores the rules governing cellular recognition of stimulatory and suppressive motifs, and the resultant modulation of the immune system. Results suggest that both CpG and suppressive ODN may find use as therapeutic agents.

Subject Keywords

General Biochemistry, Genetics and Molecular Biology, History and Philosophy of Science

URI

https://hdl.handle.net/11511/48001

Journal

Annals of the New York Academy of Sciences

DOI

https://doi.org/10.1196/annals.1281.023

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Immunotherapeutic applications of CpG ODN Gürsel, Mayda (2006-06-01) Bacterial DNA and synthetic oligodeoxynucleotides (ODN) expressing unmethylated CpG motifs stimulate the mammalian immune system to mount a rapid innate immune response. This response is characterized by the production of polyreactive IgM, immunomodulatory cytokines and chemokines. CpG ODN directly stimulate lymphocytes, natural killer cells and professional antigen-presenting cells (such as macrophages and dendritic cells). Owing to the strength and nature of this stimulation, CpG ODN are being harnessed f...
Structural Basis for EPC1-Mediated Recruitment of MBTD1 into the NuA4/TIP60 Acetyltransferase Complex Zhang, Heng; Devoucoux, Maëva; Song, Xiaosheng; Li, Li; Ayaz, Gamze; Cheng, Harry; Tempel, Wolfram; Dong, Cheng; Loppnau, Peter; Côté, Jacques; Min, Jinrong (Elsevier BV, 2020-3) MBTD1, a H4K20me reader, has recently been identified as a component of the NuA4/TIP60 acetyltransferase complex, regulating gene expression and DNA repair. NuA4/TIP60 inhibits 53BP1 binding to chromatin through recognition of the H4K20me mark by MBTD1 and acetylation of H2AK15, blocking the ubiquitination mark required for 53BP1 localization at DNA breaks. The NuA4/TIP60 non-catalytic subunit EPC1 enlists MBTD1 into the complex, but the detailed molecular mechanism remains incompletely explored. Here, we p...
Regulation of biofilm formation bymarTinSalmonellaTyphimurium Eran, Zeynep; AKÇELİK, MUSTAFA; Yazici, Betul Cansu; Özcengiz, Gülay; AKÇELİK, NEFİSE (Springer Science and Business Media LLC, 2020-07-01) In this study, we aimed at identifying the regulatory role ofmarTgene, known as the regulator ofmisL, on 15 different biofilm-related genes inS.Typhimurium 14028 strain. We also tested the strains for their ability to form biofilm and determined the adherence characteristics of the wild type and the mutant strains of the organism on Caco-2 and HEp-2 cells. For comparative analyses of the candidate genes, individual gene mutations were created via antibiotic gene cassette insertion into each gene of interest...
Effect of quercetin on mRNA and protein expressions of vitamin D metabolizing CYP27B1 and CYP24A1 in human embryonic kidney cell line (HEK-293) Durukan, Özlem; Akkulak, Merve; Özhan, Güneş; Evin, Emre; Adalı, Orhan (Wiley, 2018-07-01) Vitamin D is essential compound for life having role not only in the regulation of calcium metabolism but also in the regulation of cell proliferation, immune responses, cardiovascular homeostasis and nervous system. These wide range of actions occur with the enzymatic conversion of vitamin D to 1a,25-dihydroxyvitamin D by cytochrome P450 enzymes (CYPs). While CYP27B1 provides the synthesis of hormonally active form of Vitamin D, 1,25(OH)2D, CYP24A1 involves in catabolism of vitamin D. Quercetin, as one of ...
Investigating the malleability of RNA aptamers İlgü, Müslüm; Lamm, Monica H.; Nilsen-Hamilton, Marit (Elsevier BV, 2013-09-15) Aptamers are short, single-stranded nucleic acids with structures that frequently change upon ligand binding and are sensitive to the ionic environment. To achieve facile application of aptamers in controlling cellular activities, a better understanding is needed of aptamer ligand binding parameters, structures, intramolecular mobilities and how these structures adapt to different ionic environments with consequent effects on their ligand binding characteristics. Here we discuss the integration of biochemic...

Citation Formats

D. Klinman, R. Zeuner, H. Yamada, M. Gürsel, D. Currie, and I. Gursel, “Regulation of CpG-induced immune activation by suppressive oligodeoxynucleotides.,” Annals of the New York Academy of Sciences, pp. 112–23, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48001.