Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Regulation of biofilm formation bymarTinSalmonellaTyphimurium
Date
2020-07-01
Author
Eran, Zeynep
AKÇELİK, MUSTAFA
Yazici, Betul Cansu
Özcengiz, Gülay
AKÇELİK, NEFİSE
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
188
views
0
downloads
Cite This
In this study, we aimed at identifying the regulatory role ofmarTgene, known as the regulator ofmisL, on 15 different biofilm-related genes inS.Typhimurium 14028 strain. We also tested the strains for their ability to form biofilm and determined the adherence characteristics of the wild type and the mutant strains of the organism on Caco-2 and HEp-2 cells. For comparative analyses of the candidate genes, individual gene mutations were created via antibiotic gene cassette insertion into each gene of interest.marTgene was cloned behind an arabinose inducible BAD promoter in order to controlmarTexpression. This recombinant plasmid was transfer into each of the 15 mutant strains to investigate the level of expression of each single gene in the presence and absence ofmarTinduction. Besides determination of variations in biofilm formation by each mutant strain, the attachment characteristics of them onto Caco-2 and HEp-2 cell lines were also reported. As a result of attachments experiments on polystyrene surfaces, it was determined that the biofilm production capacity of each mutant strain decreased in a statistically significant manner (p < 0.05). QRT-PCR trials indicated that themarTgene regulates the expression of 14 genes, namelyfimA,fimD,fimF,fimH,stjB,stjC,csgA,csgD,ompC,sthB,sthE,rmbA,fliZandyaiC, in a positive manner. QRT-PCR studies were also revealed that the MarT protein positively regulates its own promoter. When the adherence characteristics of the mutant strains and the wild-type were investigated by using Caco-2 and HEp-2 cells, it was determined that the single gene mutations did have no effect on bacterial adhesion. In view of our mutational analyses and biofilm formation studies, it was concluded thatfliZ,ompC,rmbA,stjBandstjCgenes are related with biofilm formation inSalmonella,besides other cellular functions of them. Taken together, our data suggested that the regulatory role of MarT protein is not only restricted to the regulation ofmisLgene expression, but it rather acts as a general regulator on the biofilm-related genes inSalmonella.
Subject Keywords
Genetics
,
Molecular Biology
,
General Medicine
URI
https://hdl.handle.net/11511/41118
Journal
MOLECULAR BIOLOGY REPORTS
DOI
https://doi.org/10.1007/s11033-020-05573-6
Collections
Department of Biology, Article
Suggestions
OpenMETU
Core
Regulation of CpG-induced immune activation by suppressive oligodeoxynucleotides.
Klinman, DM; Zeuner, R; Yamada, H; Gürsel, Mayda; Currie, D; Gursel, I (Wiley, 2003-12-01)
Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated "CpG motifs" stimulate an innate immune response characterized by the production of cytokines, chemokines, and polyreactive Igs that promote host survival following infectious challenge. Yet CpG-driven immune activation can have deleterious consequences, such as increasing the host's susceptibility to autoimmune disease. The immunomodulatory activity of CpG DNA can be blocked by DNA containing "suppressive" motifs. This work exp...
Regulation of crystal protein biosynthesis by Bacillus thuringiensis: I. Effects of mineral elements and pH
ICGEN, Y; Icgen, BÜLENT; Özcengiz, Gülay (Elsevier BV, 2002-11-01)
Crystal protein synthesis by a local isolate of Bacillus thuringiensis was monitored and compared in association with growth and sporulation in media differing in mineral element content. Mg and Cu were the most important metals for the biosynthesis of 135 kDa and 65 kDa toxin components in that the former was essential and the latter was greatly stimulatory at 10(-6) to 10(-7) M concentration. Also the inclusion of Mn favored toxin production at concentrations ranging from 3 x 10(-4) to 10(-5) M. The omiss...
Structural Basis for EPC1-Mediated Recruitment of MBTD1 into the NuA4/TIP60 Acetyltransferase Complex
Zhang, Heng; Devoucoux, Maëva; Song, Xiaosheng; Li, Li; Ayaz, Gamze; Cheng, Harry; Tempel, Wolfram; Dong, Cheng; Loppnau, Peter; Côté, Jacques; Min, Jinrong (Elsevier BV, 2020-3)
MBTD1, a H4K20me reader, has recently been identified as a component of the NuA4/TIP60 acetyltransferase complex, regulating gene expression and DNA repair. NuA4/TIP60 inhibits 53BP1 binding to chromatin through recognition of the H4K20me mark by MBTD1 and acetylation of H2AK15, blocking the ubiquitination mark required for 53BP1 localization at DNA breaks. The NuA4/TIP60 non-catalytic subunit EPC1 enlists MBTD1 into the complex, but the detailed molecular mechanism remains incompletely explored. Here, we p...
Regulation of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources.
Içgen, Y; İçgen, Bülent; Özcengiz, Gülay (Elsevier BV, 2002-11-01)
The regulation of crystal protein production in Bacillus thuringiensis 81 by sources of carbon and nitrogen was investigated. The highest titers of toxin were obtained on sucrose, lactose and inulin which also supported sporulation. Whey and molasses were also potential carbon substrates for toxin production. Other carbohydrates including glucose, glycerol, maltose, starch and dextrin yielded lower amounts of toxin. Nitrogen sources were found to exert the most profound controls. Peptone was the best organi...
Apolipoprotein A5 polymorphisms in Turkish population: association with serum lipid profile and risk of ischemic stroke
CAN DEMİRDÖĞEN, BİRSEN; Sahin, Esra; Ozcelik, Aysun Turkanoglu; Bek, Semai; Demirkaya, Seref; Adalı, Orhan (Springer Science and Business Media LLC, 2012-12-01)
Atherosclerosis, a major cause of ischemic stroke, may be associated with variability of triglyceride (TG) levels. Apolipoprotein A5 (APOA5) genetic polymorphisms are associated with altered TG levels. The objective of this study was to investigate the coding region polymorphisms S19W (rs3135506) and G185C (rs2075291) and the promoter region polymorphism -1131T > C (rs662799) of the APOA5 gene as risk factors for ischemic stroke in Turkish population. Study group consisted of 272 ischemic stroke patients an...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
Z. Eran, M. AKÇELİK, B. C. Yazici, G. Özcengiz, and N. AKÇELİK, “Regulation of biofilm formation bymarTinSalmonellaTyphimurium,”
MOLECULAR BIOLOGY REPORTS
, pp. 5041–5050, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41118.