Real-Time PCR Investigation of the Dynamic Expression of Three "RNA Processing and Modification" Genes of Phanerochaete chrysosporium Exposed to Lead

Date

2013-01-01

Author

Tekın, Elif
İçgen, Bülent
Özcengiz, Gülay

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

187
views

0
downloads

The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 mu M lead, respectively. 50 and 100 mu M lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 mu M lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. Expression level of ATP-dependent RNA helicase was not affected from the period of exposure to this metal.

Subject Keywords

Toxicology, Pollution, Health, Toxicology and Mutagenesis, General Medicine

URI

https://hdl.handle.net/11511/48192

Journal

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY

DOI

https://doi.org/10.1007/s00128-012-0886-2

Collections

Department of Environmental Engineering, Article

Suggestions

OpenMETU
Core

Methicillin-Resistant Bacteria Inhabiting Surface Waters Monitored by mecA-Targeted Oligonucleotide Probes Seyedmonir, Elnaz; Yılmaz, Fadime; İçgen, Bülent (Springer Science and Business Media LLC, 2016-08-01) Part of a 20-60 kb staphylococcal chromosome cassette called mecA encodes low-affinity penicillin-binding protein PBP2a and causes methicillin resistance. Among all methicillin-resistant bacteria, methicillin-resistant Staphylococcus aureus is a major pathogen and main concern worldwide. Although the origin of the mecA is not very well-defined, mecA homologues are also ubiquitous in methicillin-resistant non-staphylococcal bacteria. Due to the dissemination of methicillin resistance through the transmission...
Developmental genetics and physiology of sex differentiation in vertabrates Uguz, C; İşcan, Mesude; Togan, İnci Zehra (Elsevier BV, 2003-06-01) The involvement of the Y chromosome in sex determination was determined by the development and the application of techniques for karyotyping the mammalian chromosome in 1960s. There were many reports on the particular region of the Y chromosome. Such as histocompatibility (H-Y) antigen, bandit krait minor satellite (Bkm) the zinc finger Y gene (ZFY) and the sex-determining region of the Y chromosome (SRY) which were believed to be the testis determining factors. However, converging experimental evidence hav...
Mitochondria-Targeting Selenophene-Modified BODIPY-Based Photosensitizers for the Treatment of Hypoxic Cancer Cells Karaman, Osman; Almammadov, Toghrul; Gedik, M. Emre; GÜNAYDIN, GÜRCAN; Kolemen, Safacan; Günbaş, Emrullah Görkem (Wiley, 2019-11-05) Two red-absorbing, water-soluble and mitochondria (MT)-targeting selenophene-substituted BODIPY-based photosensitizers (PSs) were realized (BOD-Se, BOD-Se-I), and their potential as photodynamic therapy (PDT) agents were evaluated. BOD-Se-I showed higher O-1(2) generation yield thanks to the enhanced heavy-atom effect, and this derivative was further tested in detail in cell culture studies under both normoxic and hypoxic conditions. BOD-Se-I not only effectively functioned under hypoxic conditions, but als...
VanA-Type MRSA (VRSA) Emerged in Surface Waters İçgen, Bülent (Springer Science and Business Media LLC, 2016-09-01) Due to the widespread occurrence of mecA-encoded methicillin resistance in Staphylococcus aureus (MRSA), treatment of staphylococcal infections is shifted to glycopeptide antibiotics like vancomycin and teicoplanin. The selective pressure of glycopeptides has eventually led to the emergence of staphylococci with increased resistance. Of great concern is vanA-encoded high level vancomycin and teicoplanin resistance in MRSA (VRSA). Therefore, this study aimed at investigating the occurrence of VRSA in surface...
Biofilms attached to Myriophyllum spicatum play a dominant role in nitrogen removal in constructed wetland mesocosms with submersed macrophytes: Evidence from N-15 tracking, nitrogen budgets and metagenomics analyses Mu, Xiaoying; Lv, Xiaoyang; Liu, Wei; Qiu, Changhao; Ma, Yu; Zhang, Songhe; Jeppesen, Erik (Elsevier BV, 2020-11-01) The mechanisms behind nitrogen removal by the submersed macrophyte-biofilm complex in wetlands remain to be fully elucidated. This study investigated the role of Myriophyllum spicatum and the biofilm on their leaves in nitrogen removal in mesocosm experiments. N-15 tracking showed that 61.9% and 30% of the N-15, respectively, was removed from the system and assimilated by the macrophyte-biofilm complex after loading with 5.4 mg L-1 N-15 labelled NH4+ for 17 days. Nitrogen budget results showed that about 0....

Citation Formats

E. Tekın, B. İçgen, and G. Özcengiz, “Real-Time PCR Investigation of the Dynamic Expression of Three “RNA Processing and Modification” Genes of Phanerochaete chrysosporium Exposed to Lead,” BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, pp. 27–33, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48192.