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Real-Time PCR Investigation of the Dynamic Expression of Three "RNA Processing and Modification" Genes of Phanerochaete chrysosporium Exposed to Lead
Date
2013-01-01
Author
Tekın, Elif
İçgen, Bülent
Özcengiz, Gülay
Metadata
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The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 mu M lead, respectively. 50 and 100 mu M lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 mu M lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. Expression level of ATP-dependent RNA helicase was not affected from the period of exposure to this metal.
Subject Keywords
Toxicology
,
Pollution
,
Health, Toxicology and Mutagenesis
,
General Medicine
URI
https://hdl.handle.net/11511/48192
Journal
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
DOI
https://doi.org/10.1007/s00128-012-0886-2
Collections
Department of Environmental Engineering, Article