Methicillin-Resistant Bacteria Inhabiting Surface Waters Monitored by mecA-Targeted Oligonucleotide Probes

Seyedmonir, Elnaz
Yılmaz, Fadime
İçgen, Bülent
Part of a 20-60 kb staphylococcal chromosome cassette called mecA encodes low-affinity penicillin-binding protein PBP2a and causes methicillin resistance. Among all methicillin-resistant bacteria, methicillin-resistant Staphylococcus aureus is a major pathogen and main concern worldwide. Although the origin of the mecA is not very well-defined, mecA homologues are also ubiquitous in methicillin-resistant non-staphylococcal bacteria. Due to the dissemination of methicillin resistance through the transmission of mecA gene among staphylococcal and non-staphylococcal bacteria inhabiting surface waters, there is a need to monitor mecA gene in these waters for public health safety. Therefore, this study aimed at monitoring mecA harboring bacteria inhabiting surface waters by using fluorescently labelled mecA-targeted oligonucleotide probes. Under the hybridization conditions of 55 % formamide and 0.020 M NaCl at 46A degrees C, the oligonucleotide probe used in the study showed high hybridization stringency to the mecA gene targeted. The strong linear relationships observed between the signal intensity and the target gene were used to assess the population dynamics of mecA harboring isolates over a 2-year-period. The results indicated that mecA-targeted oligonucleotide probes can be effectively used for in situ monitoring of methicillin resistant isolates inhabiting surface waters.


Real-Time PCR Investigation of the Dynamic Expression of Three "RNA Processing and Modification" Genes of Phanerochaete chrysosporium Exposed to Lead
Tekın, Elif; İçgen, Bülent; Özcengiz, Gülay (Springer Science and Business Media LLC, 2013-01-01)
The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 mu M lead, respectively. 50 and 100 mu M lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the...
Developmental genetics and physiology of sex differentiation in vertabrates
Uguz, C; İşcan, Mesude; Togan, İnci Zehra (Elsevier BV, 2003-06-01)
The involvement of the Y chromosome in sex determination was determined by the development and the application of techniques for karyotyping the mammalian chromosome in 1960s. There were many reports on the particular region of the Y chromosome. Such as histocompatibility (H-Y) antigen, bandit krait minor satellite (Bkm) the zinc finger Y gene (ZFY) and the sex-determining region of the Y chromosome (SRY) which were believed to be the testis determining factors. However, converging experimental evidence hav...
VanA-Type MRSA (VRSA) Emerged in Surface Waters
İçgen, Bülent (Springer Science and Business Media LLC, 2016-09-01)
Due to the widespread occurrence of mecA-encoded methicillin resistance in Staphylococcus aureus (MRSA), treatment of staphylococcal infections is shifted to glycopeptide antibiotics like vancomycin and teicoplanin. The selective pressure of glycopeptides has eventually led to the emergence of staphylococci with increased resistance. Of great concern is vanA-encoded high level vancomycin and teicoplanin resistance in MRSA (VRSA). Therefore, this study aimed at investigating the occurrence of VRSA in surface...
Radiation induced chain addition of allylbenzene to 1,4-dioxane
Nesterov, SV; Kucukyavuz, S; Önal, Ahmet Muhtar (Springer Science and Business Media LLC, 1999-06-01)
Radiation induced chain addition of allylbenzene to 1,4-dioxane has been studied by IR-, UV-, mass-, NMR- and ESR-spectrometry. Efficiency of the addition depends on the concentration of monomer and it has an optimum at C-monom<0.125M. Apparently, upper concentration limit of the reaction is caused by absorbed energy transfer on the molecule of monomer followed by energy deactivation. Radiation-chemical yields of the product are 12 and 31 molec./100 eV for 0.125M and 0.07M solution, respectively. The value ...
Genotype and allele frequencies of polymorphic CYP2E1 in the Turkish population
Ulusoy, Gulen; Arinc, Emel; Adalı, Orhan (Springer Science and Business Media LLC, 2007-10-01)
Cytochrome P4502E1 (CYP2E1) gene shows genetic polymorphisms that vary markedly in frequency among different ethnic and racial groups. We studied the genotype distributions and allele frequencies of three CYP2E1 polymorphisms: CYP2E1*5B (RsaI/PstI RFLP, C-1053T/G-1293C SNP, rs2031920 /rs3813867), CYP2E1*6 (DraI RFLP, T7632A SNP, rs6413432), and CYP2E1*7B (DdeI RFLP, G-71T SNP, rs6413420) by PCR/RFLP technique in a sample of 206 healthy subjects representing Turkish population. CYP2E1*5B polymorphism analysi...
Citation Formats
E. Seyedmonir, F. Yılmaz, and B. İçgen, “Methicillin-Resistant Bacteria Inhabiting Surface Waters Monitored by mecA-Targeted Oligonucleotide Probes,” BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, pp. 261–271, 2016, Accessed: 00, 2020. [Online]. Available: