Concentration-dependent differing actions of the nonsteroidal anti-inflammatory drug, celecoxib, in distearoyl phosphatidylcholine multilamellar vesicles

Date

2010-06-01

Author

Sade, Asli
Banerjee, Sreeparna
Severcan, Feride

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The interactions of the nonsteroidal anti-inflammatory drug, celecoxib, with 1,2-distearoyl-sn-glycero-3-phosphocholine multilamellar vesicles were studied as a function of temperature and different drug concentrations, using Fourier transform infrared spectroscopy, differential scanning calorimetry, and turbidity technique at 440 nm. Our studies reveal that celecoxib lowers the main phase-transition temperature and decreases the fluidity of the membranes at all concentrations. Celecoxib induced opposing effects on molecular order at different concentrations by increasing the ordering of the system at low concentrations and disordering it at high concentrations. Further, the drug increases the number of hydrogen bonds around the carbonyl groups at low concentrations in both phases, whereas the degree of dehydration increases at high concentrations in the gel phase. An evidence of phase separation has also been clearly observed at high concentrations. Thus, depending on the concentration used, celecoxib induces significant changes in the biophysical properties of membranes that may aid in understanding its mechanism of action.</.

Subject Keywords

Pharmaceutical Science

URI

https://hdl.handle.net/11511/48573

Journal

JOURNAL OF LIPOSOME RESEARCH

DOI

https://doi.org/10.3109/08982100903244492

Collections

Department of Biology, Article

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Citation Formats

A. Sade, S. Banerjee, and F. Severcan, “Concentration-dependent differing actions of the nonsteroidal anti-inflammatory drug, celecoxib, in distearoyl phosphatidylcholine multilamellar vesicles,” JOURNAL OF LIPOSOME RESEARCH, pp. 168–177, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48573.