Concentration dependent different action of progesterone on the order, dynamics and hydration states of the head group of dipalmitoyl-phosphatidylcholine membrane

Download

10.1155:2005:641043.pdf

Date

2005

Author

Korkmaz, Filiz
Kirbiyik, Halil
Severcan, Feride

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

128
views

76
downloads

Interactions of progesterone with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) multilamellar liposomes (MLVs) were investigated as a function of progesterone concentration at selected temperatures monitoring both the gel and liquid crystalline phase, by using Fourier Transform Infrared spectroscopy (FTIR). It has been show that the effect of progesterone on membrane dynamics is dependent on progesterone concentration. At 1 mol%, which is close to physiological level, progesterone behaves differently. At this concentration the decrease in dynamics is more noticeable. Additionally a dramatic decrease in the strength of hydrogen bonding in the interfacial region of the bilayer is also observed. When concentration increases up to 12 mol% opposite behaviour is observed at all interactions. Above 12 mol%, progesterone–DPPC interactions shows almost linear plot.

Subject Keywords

DPPC liposome, Progesterone, FTIR, Membrane fluidity, Lipid order

URI

https://hdl.handle.net/11511/51738

Journal

Spectroscopy

DOI

https://doi.org/10.1155/2005/641043

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Biophysical studies of progesterone-model membrane interactions Korkmaz, Filiz; Kırbıyık, Halil; Severcan, Feride; Department of Physics (2003) Interactions of progesterone with zwitterionic dipalmitoyl phosphotidylcholine (DPPC) multilamellar liposomes (MLVs) were investigated as a function of temperature and progesterone concentration by using three non-invasive techniques of Fourier transform infrared (FTIR) spectroscopy, turbidity at 440 nm and differential scanning calorimetry (DSC).The results show that lmol% of progesterone does not induce a significant change in the shape of thermotropic profile of DPPC. However as progesterone concentratio...
Oestrogen phospholipid membrane interactions: An FTIR study Boyar, H; Severcan, Feride (1997-06-01) Interactions of 17 beta-oestradiol (E2) with dipalmitoyl phosphatidylcholine (DPPC) multilamellar liposomes were investigated by Fourier transform infrared (FTIR) spectroscopy as a function of temperature. The investigation of the C-H stretching region and the C=0 mode reveals that E2 changes the physical properties of the DPPC bilayers by decreasing the main phase transition temperature, diminishing the pretransition, slightly disordering the system in the gel and in the liquid crystalline phase, increasin...
Effects of the non-steroidal anti-inflammatory drug celecoxib on cholesterol containing distearoyl phosphatidylcholine membranes Sade, Asli; Banerjee, Sreeparna; Severcan, Feride (2011-01-01) The effects of different concentrations of celecoxib on the acyl chain order, dynamics and the hydration status of the head group and interfacial region of model membranes containing DSPC and cholesterol were investigated in detail using Fourier transform infrared spectroscopy. Our results reveal that regardless of the presence of cholesterol, celecoxib is able to alter the physical properties of membranes. It exerts opposing effects on membrane order at high and low concentrations and decreases membrane fl...
Concentration-dependent differing actions of the nonsteroidal anti-inflammatory drug, celecoxib, in distearoyl phosphatidylcholine multilamellar vesicles Sade, Asli; Banerjee, Sreeparna; Severcan, Feride (Informa UK Limited, 2010-06-01) The interactions of the nonsteroidal anti-inflammatory drug, celecoxib, with 1,2-distearoyl-sn-glycero-3-phosphocholine multilamellar vesicles were studied as a function of temperature and different drug concentrations, using Fourier transform infrared spectroscopy, differential scanning calorimetry, and turbidity technique at 440 nm. Our studies reveal that celecoxib lowers the main phase-transition temperature and decreases the fluidity of the membranes at all concentrations. Celecoxib induced opposing ef...
Competitive effect of vitamin D-2 and Ca2+ on phospholipid model membranes: an FTIR study Toyran, N; Severcan, Feride (2003-04-01) The interaction of Ca2+, with dipalmitoyl phosphatidylcholine (DPPC) model membranes was studied in the presence and absence of vitamin D-2 by using Fourier transform infrared spectroscopy. Addition of vitamin D-2 and/or Ca2+ into pure DPPC liposomes shifts the phase transition to higher temperature, orders and decreases the dynamics of the acyl chains in both phases and does not induce hydrogen bond formation in the interfacial region. Moreover, the dynamics of the head group of the phospholipid decreases ...

Citation Formats

F. Korkmaz, H. Kirbiyik, and F. Severcan, “Concentration dependent different action of progesterone on the order, dynamics and hydration states of the head group of dipalmitoyl-phosphatidylcholine membrane,” Spectroscopy, pp. 213–219, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51738.