Disintegration Efficiency of Pulsed Electric Field Induced Effects on Onion (Allium cepa L.) Tissues as a Function of Pulse Protocol and Determination of Cell Integrity by 1H-NMR Relaxometry

Date

2010-09-10

Author

ERSUS, Seda
Öztop, Halil Mecit
MCCARTHY, Michael J.
BARRETT, Diane M.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

139
views

0
downloads

The influence of electrical pulse protocol parameters on cell rupture of onion tissues was investigated in order to improve fundamental understanding and to enhance the processing of plant tissues with pulsed electric fields (PEFs). The impact of PEF parameters on cell integrity of 20 mm dia, 4-mm thick disks of Don Victor onions (Allium cepa L.) was determined by ion leakage measurements. Electric field strength, pulse width, total pulse duration, and frequency effects were determined in relation to their effects on cell damage as a function of pulse protocol. Electric field strengths up to 500 V/cm increased the damage efficiency but there was no significant difference in efficiency beyond this field strength. Larger pulse widths increased the degree of tissue disintegration at a constant pulse number. Higher PEF efficiency was achieved with shorter pulse widths and a larger number of pulses at a constant total treatment time. Lower frequencies caused a greater degree of disintegration at constant number of pulses. 1H-NMR experiments were performed to determine the proton relaxation components of the PEF-treated onion samples and to obtain cell damage information nondestructively. Paramagnetic ion uptake by the onion sample was used to identify different proton relaxation components. Five different proton relaxation components were observed and changes in the 2 components representing different proton environments showed high correlations with ion leakage results (R2 = 0.99), indicating that T-2 distributions can be used to obtain information about cell membrane integrity in PEF-treated samples. 1H-NMR proved to be an effective method for nondestructive quantification of cell membrane rupture in onions.

Subject Keywords

Cell Disintegration, Frequency, Membrane İntegrity, Nuclear Magnetic Resonance (NMR), Onion Tissue, Pulsed Electric Fields, Pulse Widt, T-2 Distributions

URI

https://hdl.handle.net/11511/48629

Journal

JOURNAL OF FOOD SCIENCE

DOI

https://doi.org/10.1111/j.1750-3841.2010.01769.x

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Coil sensitivity map calculation using biot-savart law at 3 tesla and parallel imaging in MRI Esin, Yunus Emre; Alpaslan, Ferda Nur; Department of Computer Engineering (2017) Coil spatial sensitivity map is considered as one of the most valuable data used in parallel magnetic resonance imaging (MRI) reconstruction. In this study, a novel sensitivity map extraction method is introduced for phased-array coils. Proposed technique uses Biot-Savart law with coil shape information and low-resolution phase image data to form sensitivity maps. The performance of this method has been tested in the parallel image reconstruction task using sensitivity encoding technique. In MRI, coil sensi...
Immobilization of invertase in conducting polymer matrices Selampinar, F; Akbulut, Ural; Ozden, MY; Toppare, Levent Kamil (1997-09-01) This paper reports a novel approach in the electrode immobilization of an enzyme, invertase, by electrochemical polymerization of pyrrole in the presence of enzyme. The polypyrrole/invertase and polyamide/polypyrrole/invertase electrodes were constructed by the entrapment of enzyme in conducting matrices during electrochemical polymerization of pyrrole. This study involves the preparation and characterization of polypyrrole/invertase and polyamide/polypyrrole/invertase electrodes under conditions compatible...
Magnetohydrodynamic Flow Imaging Using Spin-Echo Pulse Sequence Eroğlu, Hasan Hüseyin; SADIGHI, MEHDI; Eyüboğlu, Behçet Murat (2019-04-24) In this study, magnetohydrodynamic (MHD) flow of conductive liquids due to injection of electrical current during magnetic resonance imaging (MRI) is investigated. A spin-echo based MRI pulse sequence is proposed to image the MHD flow. Magnetic resonance (MR) phase effects of the MHD flow is related to the MRI pulse parameters and injected current. Average velocity distributions of the MHD flow are reconstructed using the MR phase images. The method is validated by numerical simulations. The reconstruction ...
Induced Current Magnetic Resonance Electrical Conductivity Imaging With Oscillating Gradients Eroglu, Hasan H.; Sadighi, Mehdi; Eyüboğlu, Behçet Murat (2018-07-01) In this paper, induced current magnetic resonance electrical impedance tomography (ICMREIT) by means of current induction due to time-varying gradient fields of magnetic resonance imaging (MRI) systems is proposed. Eddy current and secondary magnetic flux density distributions are calculated for a numerical model composed of a z-gradient coil and a cylindrical conductor. An MRI pulse sequence is developed for the experimental evaluation of ICMREIT on a 3T MRI scanner. A relationship between the secondary ma...
Inactivation and injury of Escherichia coli O157 : H7 and Staphylococcus aureus by pulsed electric fields DAMAR, SİBEL; Bozoglu, F; Hizal, MİRZAHAN; Bayındırlı, Alev (2002-02-01) Two pathogenic microorganisms Escherichia coli O157:H7 and Staphylococcus aureus, suspended in peptone solution (0.1% w/v) were treated with 12, 14, 16 and 20 kV/cm electric field strengths with different pulse numbers up to 60 pulses. Pulsed electric field (PEF) treatment at 20 kV/cm with 60 pulses provided nearly 2 log reduction in viable cell counts of E. coli O157:H7 and S. aureus. S. aureus cells were slightly more resistant than E.coli O157:H7 cells. The results related to the effect of initial cell c...

Citation Formats

S. ERSUS, H. M. Öztop, M. J. MCCARTHY, and D. M. BARRETT, “Disintegration Efficiency of Pulsed Electric Field Induced Effects on Onion (Allium cepa L.) Tissues as a Function of Pulse Protocol and Determination of Cell Integrity by 1H-NMR Relaxometry,” JOURNAL OF FOOD SCIENCE, pp. 0–0, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48629.