Inactivation and injury of Escherichia coli O157 : H7 and Staphylococcus aureus by pulsed electric fields

Date

2002-02-01

Author

DAMAR, SİBEL
Bozoglu, F
Hizal, MİRZAHAN
Bayındırlı, Alev

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

148
views

0
downloads

Two pathogenic microorganisms Escherichia coli O157:H7 and Staphylococcus aureus, suspended in peptone solution (0.1% w/v) were treated with 12, 14, 16 and 20 kV/cm electric field strengths with different pulse numbers up to 60 pulses. Pulsed electric field (PEF) treatment at 20 kV/cm with 60 pulses provided nearly 2 log reduction in viable cell counts of E. coli O157:H7 and S. aureus. S. aureus cells were slightly more resistant than E.coli O157:H7 cells. The results related to the effect of initial cell concentration of E. coli O157:H7 on the PEF inactivation showed that more inactivation was obtained by decreasing initial cell concentration. Any possible injury by PEF was also investigated after applying 20 kV/cm electric field to the microorganisms. As a result, it was determined that there was 35.92 to 43.36% injury in E. coli O157:H7 cells, and 17.26 to 30.86% injury in S. aureus cells depending on pulse number. The inactivation results were also described by a kinetic model.

Subject Keywords

Electric field strength, S. aureus, Pulsed electric field, Pulse number, Pathogens, Modelling, Injury, Inactivation, E. coli O157 : H7

URI

https://hdl.handle.net/11511/43968

Journal

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

DOI

https://doi.org/10.1023/a:1013900222139

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Physiological changes of Escherichia coli O157:H7 and Staphylococcus aureus following exposure to high hydrostatic pressure ÇELİK, MUTLU; Yousef, Ahmed; Alpas, Hami (2013-09-01) Morphological changes and membrane integrity of Escherichia coli O157:H7 and Staphylococcus aureus cells before and after high hydrostatic pressure (HHP) treatments (200-400 MPa) and time (1-5 min), at a constant temperature (40 A degrees C), in peptone water were examined by using scanning electron microscopy (SEM) and fluorescent microscopy, respectively. SEM images showed that unpressurized cells exhibited a smooth surface appearance. E. coli O157:H7 cells exposed to pressure treatments first appeared la...
Enzymatic extraction of activated sludge extracellular polymers and implications on bioflocculation Sesay, Ml; Özcengiz, Gülay; Sanin, Faika Dilek (Elsevier BV, 2006-04-01) This study examines enzyme hydrolysis, a mild, effective, but a rarely used method of extracellular polymer extraction, in removing polymers from mixed culture activated sludge flocs. Two carbohydrate specific enzymes (a-amylase and cellulase) and a protein specific enzyme (proteinase) are used during the study. First, the kinetic aspect is investigated, then enzyme dose optimization is carried out on laboratory grown activated sludge samples cultured at solids retention times (SRT) of 4 and 20 days. A more...
Dielectric Characterization of Imatinib Resistant K562 Leukemia Cells through Electrorotation with 3-D Electrodes Bahrieh, G.; Koydemir, H. Ceylan; Erdem, Murat; Ozgur, E.; Gündüz, Ufuk; Külah, Haluk (2013-11-06) This study reports the use of electrorotation (ER) technique within a micro-fabricated device with 3D electrodes for dielectric characterization of Imatinib-resistant K562 (K562/IMA-0.2) human leukemic cells. The ER devices with 3D quadruple electrodes (30 mu m in height) were used in order to eliminate the fringing field effect on the rotation of cells. To induce the rotational moment on the cells, signals in phase quadrature were applied to the triangular electrodes. The rotation of cells measured at the ...
Disintegration Efficiency of Pulsed Electric Field Induced Effects on Onion (Allium cepa L.) Tissues as a Function of Pulse Protocol and Determination of Cell Integrity by 1H-NMR Relaxometry ERSUS, Seda; Öztop, Halil Mecit; MCCARTHY, Michael J.; BARRETT, Diane M. (2010-09-10) The influence of electrical pulse protocol parameters on cell rupture of onion tissues was investigated in order to improve fundamental understanding and to enhance the processing of plant tissues with pulsed electric fields (PEFs). The impact of PEF parameters on cell integrity of 20 mm dia, 4-mm thick disks of Don Victor onions (Allium cepa L.) was determined by ion leakage measurements. Electric field strength, pulse width, total pulse duration, and frequency effects were determined in relation to their ...
Electrochemical polymerization and characterization of polyether-substituted aniline derivatives Tirkeş, Süha; Oenal, Ahmet M. (Wiley, 2007-08-01) New compounds consisting of aniline units linked by polyether bridges have been synthesized and their electrochemical polymerization was performed via constant potential electrolysis and cyclic voltammetry in an aqueous solution containing 3.0 moI L-1 H2SO4. Chemical polymerization was carried out using (NH4)(2)S2O8 as oxidizing agent. It was found that both methods gave the same polymer product without any cleavage of the polyether bridge between aniline rings. The polymers were characterized using the Fou...

Citation Formats

S. DAMAR, F. Bozoglu, M. Hizal, and A. Bayındırlı, “Inactivation and injury of Escherichia coli O157 : H7 and Staphylococcus aureus by pulsed electric fields,” WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, pp. 1–6, 2002, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43968.