Evaluation of abiotic stress tolerance and physiological characteristics of potato (Solanum tuberosum L. cv. Kennebec) that heterologously expresses the rice Osmyb4 gene

Date

2014-05-01

Author

Aydin, Gulsum
Yucel, Meral
Chan, Ming-Tsair
Öktem, Hüseyin Avni

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

98
views

0
downloads

MYB transcription factors are involved in diverse biochemical and physiological processes, including hormone signaling, defense, and stress responses. In the present study, we developed a transgenic potato (Solanum tuberosum L. cv. Kennebec) expressing the rice Osmyb4 gene, which encodes the transcription factor MYB4. The transgene was under the control of either the constitutive CaMV35S promoter or the stress-induced Arabidopsis COR15a promoter. The potential involvement of MYB4 in certain physiological processes and the abiotic stress response in the potato was evaluated. The transgenic plants did not exhibit growth retardation, and they showed no significant difference (P < 0.05) in tuber yield from that of non-transgenic wild-type plants. Although the chlorophyll a and b as well as the anthocyanin contents of the six transgenic lines were similar to those of the wild type, the transgenic line S2 presented a significantly higher carotenoid content. The total sugar contents of the lines S2 and M48 were significantly higher than that of the wild-type plants. S2 and M48 were significantly more tolerant of salinity than the wild type, according to measured growth parameters. Transgenic plants grown under a high concentration of boric acid (3 mM) exhibited greater survival rates than non-transgenic control plants. On the other hand, the transgenic plants did not show an improvement in freezing tolerance. Overall, our results indicated that MYB4 may affect diverse processes such as carotenoid biosynthesis, sugar metabolism, and salinity tolerance in potato, and that it may be an upstream regulatory element of these processes.

Subject Keywords

Biotechnology, Plant Science

URI

https://hdl.handle.net/11511/48916

Journal

PLANT BIOTECHNOLOGY REPORTS

DOI

https://doi.org/10.1007/s11816-014-0322-7

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Comparison of benzaldehyde lyase production capacity in recombinant Escherichia coli and recombinant Bacillus species Kaya, Hande; Çalık, Pınar; Department of Chemical Engineering (2006) In this study, the benzaldehyde lyase (BAL, EC 4.1.2.38) production in E. coli BL21 (DE3) pLySs as intracellular and in Bacillus species as extracellular were investigated, and comparison of the production capacity of the enzyme in the developed recombinant microorganisms were compared. For this purpose, firstly, PCR amplified bal gene was cloned into pRSETA vector which is under the control of strong T7 promoter and expressed in E. coli BL21 (DE3) pLysS strain. With developed recombinant E. coli BL21 (DE3)...
Cutting edge: Role of toll-like receptor 9 in CpG DNA-induced activation of human cells Takeshita, F; Leifer, CA; Gursel, I; Ishii, KJ; Takeshita, S; Gürsel, Mayda; Klinman, DM (The American Association of Immunologists, 2001-10-01) Unmethylated CpG motifs present in bacterial DNA stimulate a rapid and robust innate immune response. Human cell lines and PBMC that recognize CpG DNA express membrane-bound human Toll-like receptor 9 (hTLR9). Cells that are not responsive to CpG DNA become responsive when transfected with hTLR9. Expression of hTLR9 dramatically increases uptake of CpG (but not control) DNA into endocytic vesicles. Upon cell stimulation, hTLR9 and CpG DNA are found in the same endocytic vesicles. Cells expressing hTLR9 are ...
Recombinant therapeutic protease production by Bacillus sp. Korkmaz, Nuriye; Çalık, Pınar; Department of Chemical Engineering (2007) The first aim of this study is the development of extracellular recombinant therapeutic protease streptokinase producing Bacillus sp., and the second aim is to determine fermentation characteristics for streptokinase production. In this context, the signal (pre-) DNA sequence of B.licheniformis (DSM1969) extracellular serine alkaline protease enzyme gene (subC: Acc. No. X03341) was ligated to 5’ end of the streptokinase gene (skc: Acc. No. S46536) by SOE (Gene Splicing by Overlap Extension) method through P...
Suppressive oligodeoxynucleotides inhibit Th1 differentiation by blocking IFN-gamma- and IL-12-mediated signaling. Shirota, H; Gürsel, Mayda; Klinman, DM (The American Association of Immunologists, 2004-10-15) Repetitive TTAGGG motifs present at high frequency in mammalian telomeres can suppress Th1-mediated immune responses. Synthetic oligonucleotides (ODN) containing TTAGGG motifs mimic this activity and have proven effective in the prevention/ treatment of certain Th1-dependent autoimmune diseases. This work explores the mechanism by which suppressive ODN block the induction of Th1 immunity. Findings indicate that these ODN inhibit IFN- -induced STAT1 phosphorylation and IL-12- induced STAT3 and STAT4 phosphor...
Development of PCR methods for detection and quantification of genetically modified maize Jabbari Farhoud, Houman; Gültekin, Güzin Candan; Department of Biotechnology (2010) This study describes development of methods for screening, identification and quantification of genetic modifications in maize samples. Totally 88 maize samples were collected randomly throughout Turkey in three years from 2006 to 2008 and were analyzed. Two maize samples that were detected as GM positive in previous studies were selected as positive controls. Following the DNA extraction by manual CTAB method, conventional PCR methods were employed for screening of genetic modifications in samples by detec...

Citation Formats

G. Aydin, M. Yucel, M.-T. Chan, and H. A. Öktem, “Evaluation of abiotic stress tolerance and physiological characteristics of potato (Solanum tuberosum L. cv. Kennebec) that heterologously expresses the rice Osmyb4 gene,” PLANT BIOTECHNOLOGY REPORTS, pp. 295–304, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48916.