Rapid monitoring of diabetes-induced lipid peroxidation by Fourier transform infrared spectroscopy: Evidence from rat liver microsomal membranes

Severcan, F
Gorgulu, G
Gorgulu, ST
Güray, Nülüfer Tülün
Increased oxidative stress is the consequence of either enhanced reactive oxygen species (ROS) production or attenuated ROS scavenging capacity, resulting in tissue damage that in most instances is assessed by the measurement of lipid peroxides. In the current study, diabetes-induced lipid peroxidation in rat liver microsomal membranes was investigated by Fourier transform infrared (FT-IR) spectroscopy at different temperatures. The olefinic (=CH) band at 3012 cm(-1) was used to probe diabetes-induced lipid peroxidation. The intensity and area values of this band of diabetic samples were found to be increased significantly (P < 0.05) compared with nondiabetic samples. The increase in olefinic band intensity is attributed mainly to the lipid peroxidation end products. The results of the FT-IR study were found to be in agreement with biochemical studies that revealed a significant increase in malondialdehyde levels of diabetic samples compared with control samples (P < 0.05) using the thiobarbituric acid test. (c) 2005 Elsevier Inc. All rights reserved.


BASAGA, HS (Canadian Science Publishing, 1990-07-01)
Toxic free radicals can be produced by many reactions required for the maintenance of normal metabolism and the production of energy in the cell. The reactivity of both primary and secondary radicals with biomolecules and in whole tissue systems is of interest, not only because of their importance in radiobiology but also because of the role these species play in toxicity and various disorders. Oxidant stress is known to increase the production of free radicals. In the presence of metals, especially iron, t...
The effect of cysteine-43 mutation on thermostability and kinetic properties of citrate synthase from Thermoplasma acidophilum
Kocabıyık, Semra; Russel, RJM; Danson, MJ; Hough, DW (Elsevier BV, 1996-07-05)
In this study, we have substituted serine-43 by cysteine in the recombinant citrate synthase from a moderately thermophilic Archaeon Thermoplasma acidophilum, for site-specific attachment of labels and have investigated the effects of this mutation on the biochemical properties and thermal stability of the enzyme. Both wild-type and the mutant enzymes were purified to homogenity using affinity chromatography on Matrex Gel Red A. The mutant Thermoplasma citrate synthase is very similar to wild-type citrate s...
Vitamin E Decreases the Order of the Phospholipid Model Membranes in the Gel Phase: An FTIR Study
Severcan, Feride (Portland Press Ltd., 1997-4-1)
The effect of α-tocopherol on the frequency of the CH2 stretching bands of infrared spectra of dipalmitoylphosphotidylcholine multibilayers has been investigated, both in H2O and 2H2O buffer, to determine the reason for the discrepancy in the literature between the results of different spectroscopic techniques relating to the effect of α-tocopherol on membrane order in the gel phase. In contrast to previous FTIR studies, the present FTIR results indicate that αT increases the frequencies of the CH2 stretchi...
Inositol phospholipids activate plasma membrane ATPase in plants
Memon, Abdul Razaque; Chen, Qiuyun; Boss, Wendy F. (Elsevier BV, 1989-8)
Phosphatidylinositol-4-monophosphate and phosphatidylinositol-4,5-bisphosphate increased the activity of the vanadate-sensitive ATPase associated with plasma membranes isolated from both sunflower hypocotyls and carrot suspension culture cells. The response was not due to the metabolism of the polyphosphoinositides since diacylglycerol, inositol-1,4-bisphosphate, inositol-1,4,5-trisphosphate, glycerophosphoinositol monophosphate and glycerophosphoinositol bisphosphate had no effect. These data suggest that ...
An alternative supporting electrolyte for enzyme immobilization in conducting polymers
Kiralp, Senem; Balik, Balam; KARATAŞ, SEVİM; Toppare, Levent Kamil; Gungorb, Atilla (Elsevier BV, 2008-03-01)
In this study an alternative supporting electrolyte was used in enzyme immobilization. Invertase was studied to observe the effect of the supporting electrolyte. Sulfonated poly(arylene ether sulfone) was used as the supporting electrolyte during the electrolysis of pyrrole. The results show that the polymeric supporting electrolyte can be used instead of sodium dodecyl sulfate.
Citation Formats
F. Severcan, G. Gorgulu, S. Gorgulu, and N. T. Güray, “Rapid monitoring of diabetes-induced lipid peroxidation by Fourier transform infrared spectroscopy: Evidence from rat liver microsomal membranes,” ANALYTICAL BIOCHEMISTRY, pp. 36–40, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/49031.