Vitamin E Decreases the Order of the Phospholipid Model Membranes in the Gel Phase: An FTIR Study

Severcan, Feride
The effect of α-tocopherol on the frequency of the CH2 stretching bands of infrared spectra of dipalmitoylphosphotidylcholine multibilayers has been investigated, both in H2O and 2H2O buffer, to determine the reason for the discrepancy in the literature between the results of different spectroscopic techniques relating to the effect of α-tocopherol on membrane order in the gel phase. In contrast to previous FTIR studies, the present FTIR results indicate that αT increases the frequencies of the CH2 stretching bands in the gel phase, which implies an increase in the number of gauche conformers (increase in disordering), which is in agreement with other ESR and NMR spectroscopic studies.
Bioscience Reports


IR and turbidity studies of vitamin E-cholesterol-phospholipid membrane interactions
Severcan, Feride; Kazanci, Nadide; Baykal, Ülkü; Süzer, Şefik (Portland Press Ltd., 1995-8-1)
Binary and tertiary mixture of α-tocophenol, cholesterol and dimyristoylphosphatidylcholine in the form of multilamellar liposomes were investigated by Fourier Transform Infrared and visible spectroscopy. Results of the FTIR and turbidity experiments indicate that α T decreases or diminishes the effect of cholesterol on the frequency and the bandwidth of the C-H stretching, CH2 scissoring and C=O stretching bands in FTIR spectra and the turbidity measurements (recorded as absorbance values at 440 nm) in pho...
Vitamin E Derivative with Modified Side Chain Induced Apoptosis by Modulating the Cellular Lipids and Membrane Dynamics in MCF7 Cells
Gok, Seher; Kuzmenko, Oleksandr; Babinskyi, Andrii; Severcan, Feride (Springer Science and Business Media LLC, 2021-01-01)
The vitamin E derivative with side chain modification (TC6OAc) has been shown to possess anticancer activity in our earlier in vivo studies. It was hypothesized that, as Vitamin E (VE) and VE derivative are fat soluble lipophilic molecules, they exert their function by modulating the lipid metabolism and related pathways. This study aimed to evaluate the cellular impact of this VE derivative (2,5,7,8-Tetramethyl-2-(4 '-Methyl-3 '-Pentenyl)-6-Acetoxy Chromane-TC6OH), using alpha-tocopherol as a reference com...
BASAGA, HS (Canadian Science Publishing, 1990-07-01)
Toxic free radicals can be produced by many reactions required for the maintenance of normal metabolism and the production of energy in the cell. The reactivity of both primary and secondary radicals with biomolecules and in whole tissue systems is of interest, not only because of their importance in radiobiology but also because of the role these species play in toxicity and various disorders. Oxidant stress is known to increase the production of free radicals. In the presence of metals, especially iron, t...
Amino acid substitutions in the subunit interface enhancing thermostability of Thermoplasma acidophilum citrate synthase
Erduran, I; Kocabıyık, Semra (Elsevier BV, 1998-08-19)
We have used citrate synthase from Thermoplasma (Tp.) acidophilum as a thermostable model system to investigate the role of hydrophobic interactions in dimer interface for maintaining high temperature stability. Three mutant enzymes were constructed by single amino acid substitutions in the interface helices: Ala97 --> Ser, Ala104 --> Thr, and Gly209 --> Ala. All of the mutations enhanced the thermostability of Tp. citrate synthase, while improving its catalytic properties (K-m, V-max, and specific activity...
Vitamin D-2 at high and low concentrations exert opposing effects on molecular order and dynamics of dipalmitoyl phosphatidylcholine membranes
Kazanci, N; Toyran, N; Haris, PI; Severcan, Feride (2001-01-01)
Fourier Transform Infrared spectroscopic studies show that low concentrations of vitamin D-2 (1 and 3 mol %) does not induce significant change in the overall shape of the thermotropic profile of dipalmitoyl phosphatidylcholine (DPPC) membrane. In contrast, at higher concentrations of vitamin D-2 (9 and 12 mol %), the phase transition shifts to lower temperatures and a significant broadening in the phase transition curve is also observed. Low concentration of vitamin D-2 decreases the frequency of the CH2 s...
Citation Formats
F. Severcan, “Vitamin E Decreases the Order of the Phospholipid Model Membranes in the Gel Phase: An FTIR Study,” Bioscience Reports, pp. 231–235, 1997, Accessed: 00, 2020. [Online]. Available: