Roles of detoxification enzymes in pyrethroid resistance of Helicoverpa armigera from Turkey

Konus, Metin
UgurIci, Sakine
İşcan, Mesude
Abstracts / Toxicology Letters 180S (2008) S32–S246S169Helicoverpa armigera, cotton bollworm, is a polyphagous insect. Itis one of the most important preharvest pests for economicallyimportant crops including cotton, legumes, and vegetables.In this study, midguts ofH. armigeraobtained from Adana,Antalya, C ̧ anakkale and Mardin field populations were used. GSTactivities were determined using 1-chloro-2,4-dinitrobenzene asa substrate. Adana and Antalya field populations showed statisti-cally significant (p< 0.05), 2.4 and 2.9 fold increase, respectively,compared to susceptible population. General esterase activitymeasurements were done with Adana, Antalya and susceptiblepopulations by usingp-nitro phenyl acetate as a substrate. Adanapopulation showed statistically significant increase (p< 0.05); nev-ertheless, a small increment (1.29 fold) was found as compared tosusceptible population. Although, Antalya population did not showstatistically significant increase (p> 0.05) compared to susceptiblepopulation, it exhibited tendency to increase.The role of cytochrome P-450 monooxygenases (CYP6B6,CYP6B7, and CYP9A12) in pyrethroid resistance developmentanalyzed using Reverse Transcriptase-PCR method. In these exper-iments, C ̧ anakkale and Mardin field populations ofH. armigerawere used. Some individuals in both field populations exhibitedslight increases in gene expressions of CYP6B7 and CYP9A12 genescompared to susceptible individuals. CYP6B6 gene expressionwas not detected in susceptible strain; nonetheless, expressionof the same gene was observed in some of the individuals fromMardin and C ̧ anakkale field populations. Increased GST activity inconjunction with activation of CYP6B6 appears to play a role in theresistance of TurkishH. armigerain monitored field


Effect of mercury, cadmium, nickel, chromium and zinc on kinetic properties of NADPH-cytochrome P450 reductase purified from leaping mullet (Liza saliens)
Bozcaarmutlu, Azra; Arinc, Emel (Elsevier BV, 2007-04-01)
Information on the mechanism of metal ion inhibition of NADPH-cytochrome P450 reductase is limited. The purpose of the present paper was to elucidate in vitro effect of Hg(+2), Cd(+2), Ni(+2), Cr(+3) and Zn(+2) ions on the purified mullet NADPH-cytochrome P450 reductase. NADPH-cytochrome P450 reductase was purified from detergent-solubilized liver microsomes from leaping mullet (Liza saliens). All of the metal ions caused inhibition of the enzyme activity except Zn(+2). At 50 mu M metal concentration, Hg(+2...
Investigation of the role of glutathione S-transferase isozymes in pyrethroid resistance of Helicoverpa armigera in Turkey
Konus, Metin; Ugurlu, Sakine; İşcan, Mesude (2009-09-13)
Helicoverpa armigera, cotton bollworm, is one of the insects causing severe yield loss of economically important crops including cotton, legumes and vegetables. It has developed resistance against the pyrethroid insecticides that have been introduced into market. Resistance against pyrethroids have been reported in H. armigera populations at various parts of the world, however, except 1-chloro-2,4-dinitrobenzene substrate (CDNB), almost no any other substrates used for analysis of the role of glutathione S-...
Effects of anaerobic digestion enhanced by ultrasound pretreatment on the fuel properties of municipal sludge
Çelebi, Emrehan Berkay; Aksoy, Ayşegül; Sanin, Faika Dilek (Springer Science and Business Media LLC, 2020-05-01)
In this study, effects of ultrasound pretreatment on combustion characteristics and elemental composition of municipal sludge were examined for energy-based evaluation of sludge pretreatment. Waste activated sludge (WAS) from a municipal wastewater treatment plant was pretreated with ultrasound at varying durations and was subjected to anaerobic digestion in a biochemical methane potential (BMP) assay. Changes in gas production rates, calorific value (CV), elemental compositions, and ash contents of sludge ...
Characterization of aldrin epoxidation in flathead mullet (Mugil cephalus) liver microsomes: Involvement of CYP3A
BOZCAARMUTLU, AZRA; Turna, S.; Erden, C. Sapmaz; Yenisoy-Karakas, S.; Arinc, E. (2011-08-28)
Toxic organochlorine pesticides, aldrin and its metabolite dieldrin, have been reported to contaminate the aquatic environment highly. In the present study, epoxidation reaction of aldrin to dieldrin was studied in mullet liver microsomes and contribution of cytochrome P450 isozyme(s) was determined by using specific cytochrome P450 inhibitors and substrate. Flathead mullet samples (Mugil cephalus) were caught from the West Black Sea Region of Turkey. Fish liver microsomes were prepared by differential cent...
Production of neutral and alkaline extracellular proteases by the thermophilic fungus, Scytalidium thermophilum, grown on microcrystalline cellulose
Ifrij, IH; Ögel, Zümrüt Begüm (Springer Science and Business Media LLC, 2002-07-01)
Extracellular proteases produced by Scytalidium thermophilum, grown on microcrystalline cellulose, were most active at pH 6.5-8 and 37-45 degreesC when incubated for 60 min. Highest protease activity was at day 3 where endoglucanase activity was low. Protease activity measurements with and without the protease inhibitors, p-chloromercuribenzoate, PMSF, antipain, E-64, EDTA and pepstatin A, suggest production of thiol-containing serine protease and serine proteases. Endoglucanase and Avicel-adsorbable endogl...
Citation Formats
M. Konus, S. UgurIci, and M. İşcan, “Roles of detoxification enzymes in pyrethroid resistance of Helicoverpa armigera from Turkey,” 2008, vol. 180, Accessed: 00, 2020. [Online]. Available: