Ionizing Radiation Induces Structural and Functional Damage on the Molecules of Rat Brain Homogenate Membranes: A Fourier Transform Infrared (FT-IR) Spectroscopic Study

Date

2015-01-01

Author

Demir, Pinar
Akkaş, Sara Banu
Severcan, Mete
Zorlu, Faruk
Severcan, Feride

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

186
views

0
downloads

Humans can be exposed to ionizing radiation, due to various reasons, whose structural effects on biological membranes are not well defined. The current study aims to understand the ionizing radiation-induced structural and functional alterations in biomolecules of brain membranes using Fourier transform infrared (FT-IR) spectroscopy using rat animal models. For this purpose, 1000 cGy of ionizing radiation was specifically directed to the head of Sprague Dawley rats. The rats were decapitated after 24 h. The results revealed that the lipid-to-protein ratio decreased and that irradiation caused lipid peroxidation and increases in the amounts of olefinic =CH, carbonyl, and methylene groups of lipids. In addition, ionizing radiation induced a decrease in membrane fluidity, disordering of membrane lipids, strengthening of the hydrogen bonding of the phosphate groups of lipid head-groups, and weakening in the hydrogen bonding of the interfacial carbonyl groups of lipids. Radiation further caused significant decrements in the α-helix and turns, and significant increments in the β-sheet and random coil contents in the protein structure. Hierarchical cluster analyses, performed in the whole region (3030–1000 cm–1), lipid (3030–2800 cm–1), and protein (1700–1600 cm–1) regions separately, successfully differentiated the control and irradiated groups of rat brain membranes and showed that proteins in the membranes are affected more than lipids from the damages induced with ionizing radiation. As a result, the current study showed that FT-IR spectroscopy can be used successfully as a novel method to monitor radiation-induced alterations on biological membranes

Subject Keywords

Instrumentation, Spectroscopy

URI

https://hdl.handle.net/11511/52142

Journal

APPLIED SPECTROSCOPY

DOI

https://doi.org/10.1366/13-07154

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Concentration-Based Measurement Studies of L-Tryptophan Using Terahertz Time-Domain Spectroscopy (THz-TDS) Ozer, Zeynep; Gok, Seher; Altan, Hakan; Severcan, Feride (SAGE Publications, 2014-01-01) L-Tryptophan is an extremely important amino acid for a variety of biological functions in living organisms. In this study we were able to measure changes in the concentration of L-tryptophan when incorporated into pellets with polyethylene as a host. The changes were measured both through the characteristic absorption bands of the C11 and C12 bonds in the low terahertz frequency range and using changes in the refractive index where pellets with higher concentrations of L-tryptophan showed higher refractive...
Time resolved spectroscopy of laser induced air plasma Kurt, Mustafa; Esendemir, Akif; Department of Physics (2007) The laser beam interaction with matter and the plasma generation have been studied for many years. In some applications what is really important is to understand the composition and the temporal evolution of the species in the interested medium. In this thesis, time resolved optical spectroscopy was employed to understand the evolution of the plasma which is produced by interaction of Infrared (1.064 m) laser beam with air. In this thesis, a new technique is suggested to analyze the time evolution of laser ...
Ultra-High Precision Radiation Dosimetry via Laser Bleaching the Color Centers in Fast Recovery Optical Fiber Sensors Ghahrizjani, Reza Taheri; Ameri, Mohsen; Jahanbakhsh, Hossein; Sadeghi, Hossein; Mohajerani, Ezeddin (Institute of Electrical and Electronics Engineers (IEEE), 2020-06-01) High energy electromagnetic radiation or heavy charged particles are extensively used for medical treatment of certain cancers. Precise prediction or measurements of energy deposition of any types of radiation on tissue is critical in radiation therapy. In this research, the sensitivity of optical fiber dosimeters specifically doped with phosphor (P), Germanium (Ge) and Erbium (Er) are analyzed using Co-60 source at 169 cGy/min. The sensors are calibrated and the values of radiation induced attenuation (RIA...
Evaluation of disseminated candidiasis on an experimental animal model: A Fourier transform infrared study Dogan, Ayca; Ergen, Kivanc; Budak, Fatma; Severcan, Feride (SAGE Publications, 2007-02-01) Invasive candidiasis, defined as candidemia and disseminated candidiasis, is the most common fungal infection in hospitalized patients. In the current study, we used Fourier transform infrared (FT-IR) spectroscopy as a rapid, non-perturbing technique to investigate the effects of disseminated candidiasis on mouse liver tissues at the molecular level. The results revealed that the infection caused compositional changes in the tissues by decreasing the lipid content and the ratio of the saturated lipids to un...
Plasma Sensing using THz Waves, Altan, Hakan (Springer, 2011-01-01) Terahertz (THz) and Mid-Infrared (MIR) radiation (TERA-MIR) can be transmitted through nearly any material without causing biological harm. Novel and rapid methods of detection can be created with devices operation in these spectral ranges allowing scanning for weapons, detecting hidden explosives (including plastic landmines), controlling the quality of food and a host of other exciting applications. This book focuses on mathematical and physical aspects of the field, on unifying these two spectral domai...

Citation Formats

P. Demir, S. B. Akkaş, M. Severcan, F. Zorlu, and F. Severcan, “Ionizing Radiation Induces Structural and Functional Damage on the Molecules of Rat Brain Homogenate Membranes: A Fourier Transform Infrared (FT-IR) Spectroscopic Study,” APPLIED SPECTROSCOPY, pp. 154–164, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/52142.