Evaluation of disseminated candidiasis on an experimental animal model: A Fourier transform infrared study

Dogan, Ayca
Ergen, Kivanc
Budak, Fatma
Severcan, Feride
Invasive candidiasis, defined as candidemia and disseminated candidiasis, is the most common fungal infection in hospitalized patients. In the current study, we used Fourier transform infrared (FT-IR) spectroscopy as a rapid, non-perturbing technique to investigate the effects of disseminated candidiasis on mouse liver tissues at the molecular level. The results revealed that the infection caused compositional changes in the tissues by decreasing the lipid content and the ratio of the saturated lipids to unsaturated lipids. An increase in the lipid/protein ratio was also observed. In addition, investigation of the olefinic band at 3014 cm(-1), showed that lipid peroxidation took place in the infected samples. These results indicate that FT-IR spectroscopy is a promising technique for the evaluation and diagnosis of disseminated candidiasis.


Ionizing Radiation Induces Structural and Functional Damage on the Molecules of Rat Brain Homogenate Membranes: A Fourier Transform Infrared (FT-IR) Spectroscopic Study
Demir, Pinar; Akkaş, Sara Banu; Severcan, Mete; Zorlu, Faruk; Severcan, Feride (SAGE Publications, 2015-01-01)
Humans can be exposed to ionizing radiation, due to various reasons, whose structural effects on biological membranes are not well defined. The current study aims to understand the ionizing radiation-induced structural and functional alterations in biomolecules of brain membranes using Fourier transform infrared (FT-IR) spectroscopy using rat animal models. For this purpose, 1000 cGy of ionizing radiation was specifically directed to the head of Sprague Dawley rats. The rats were decapitated after 24 h. The...
Development and analysis of controlled release polymeric rods containing vancomycin
Tağıt, Oya; Hasırcı, Vasıf Nejat; Department of Biotechnology (2005)
Antibiotic use is a vital method for the treatment of most diseases involving bacterial infections. Unfortunately, in certain cases these agents are not effective in treatments against diseases for either some limitation in antibiotic usage because of the side effects or some distribution problems caused by physiological or pathological barriers in the body. Such problems are thought to be minimized by development of controlled release systems which involve implantation of antibiotic loaded polymeric system...
Optimization of microwave-assisted extraction of phenolic compounds from tomato: Characterization by FTIR and HPLC and comparison with conventional solvent extraction
Baltacıoğlu, Hande; Baltacıoğlu, Cem; Okur, İlhami; Tanrıvermiş, Arif; Yalıç, Muharrem (Elsevier BV, 2021-03-01)
© 2021 Elsevier B.V.In the extraction of phenolic compounds from tomato, Microwave-Assisted Extraction (MAE) was used as a novel technology in this study and compared to conventional solvent extraction (CSE). The optimal extraction conditions for tomato were determined using the multi-level factorial design. For this purpose, different powers (360, 600 and 900 W) and times (30, 60 and 90 s) were used for MAE and also different temperatures (40, 50 and 60 °C) and times (10, 20 and 30 min) were used for CSE. ...
Characterization and genomic analysis of a novel bacteriophage against methicillin-resistant staphylococcus aureus
Çotak, Medine; Akkaya, Mahinur S.; Karahan, Zeynep Ceren; Department of Biotechnology (2019)
Methicillin resistant Staphylococcus aureus (MRSA) is one of the most frequent hospital and community acquired infectious agents causing severe diseases. Bacteriophages may offer a solution to treat the bacterial infections that are not responding to classical antibiotics. In this context, a new lytic phage, named as vB_SauM-MikSA913, was isolated from sewage treatment center in Samsun (Turkey). MRSA clinical strains obtained from a local hospital were used as propagating hosts while searching for the lytic...
The characterization of bacteria with Fourier Transform Infrared (FTIR) spectroscopy
Garip, Şebnem; Severcan, Feride; Department of Biology (2005)
New and rapid techniques for the characterization and identification of bacteria would have an important role in clinical microbiology and in food analysis because of an increasing prevalence of infectious diseases and In this work we carried out two approaches. In the first study the characterization and differentiation of mesophilic and thermophilic bacteria were investigated by using Fourier Transform Infrared (FTIR) Spectroscopic technique. In the second study, we investigated the characterization and i...
Citation Formats
A. Dogan, K. Ergen, F. Budak, and F. Severcan, “Evaluation of disseminated candidiasis on an experimental animal model: A Fourier transform infrared study,” APPLIED SPECTROSCOPY, pp. 199–203, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57135.