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Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1
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10.1242:jcs.234401.pdf
Date
2019-10-10
Author
Papini, Diana
Fant, Xavier
Ogawa, Hiromi
Desban, Nathalie
Samejima, Kumiko
Feizbakhsh, Omid
Askin, Bilge
Ly, Tony
Earnshaw, William C.
Ruchaud, Sandrine
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Timely and precise control of Aurora B kinase, the chromosomal passenger complex (CPC) catalytic subunit, is essential for accurate chromosome segregation and cytokinesis. Post-translational modifications of CPC subunits are directly involved in controlling Aurora B activity. Here, we identified a highly conserved acidic STD-rich motif of INCENP that is phosphorylated during mitosis in vivo and by Plk1 in vitro and is involved in controlling Aurora B activity. By using an INCENP conditional-knockout cell line, we show that impairing the phosphorylation status of this region disrupts chromosome congression and induces cytokinesis failure. In contrast, mimicking constitutive phosphorylation not only rescues cytokinesis but also induces ectopic furrows and contractile ring formation in a Plk1- and ROCK1-dependent manner independent of cell cycle and microtubule status. Our experiments identify the phospho-regulation of the INCENP STD motif as a novel mechanism that is key for chromosome alignment and cytokinesis.
Subject Keywords
Cell Biology
,
CPC
,
INCENP
,
Furrow initiation
,
Aurora B
,
Plk1
,
Mitosis
URI
https://hdl.handle.net/11511/52152
Journal
Journal of Cell Science
DOI
https://doi.org/10.1242/jcs.234401
Collections
Department of Biology, Article