In planta determination of GaMyb transcription factor as a target of pathogen induced microRNA, mir159

Akkaya, Mahinur
Dagdas, Gulay Gok
Dagdas, Yasin F.
One of the molecular mechanisms underlying the plant–pathogen interactions is the regulation of gene expressions in plant responses by microRNAs which are either stimulated or silenced against pathogen attacks. Among the plant miRNAs, we found that mir159 is one of which that differentially expressed upon Blumeria graminis f. sp. hordei (Bgh) infected resistant and susceptible barley lines. The study aims to confirm its role in regulating one of its putative target genes, GaMyb transcription factor, in vivo. Thus, both mir159 and GaMyb genes were co-infiltrated into tobacco as constructs of pEarlyGate100 and GFP expressing pEarlyGate103, respectively, via agrobacterium transient transformation. Our results suggest that mir159 indeed reduces the expression of GaMyb transcription factor by which, for the first time, confirm it being a biological target of mir159. To further confirm the induced expression level and the biological role of mir159 in susceptible barley by virulent Bgh pathogen infection, the differential level of disease development should be investigated in a susceptible line of barley after the over expression and/or the silencing of the gene of mir159.


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Citation Formats
M. Akkaya, G. G. Dagdas, and Y. F. Dagdas, “In planta determination of GaMyb transcription factor as a target of pathogen induced microRNA, mir159,” 2011, vol. 22, Accessed: 00, 2020. [Online]. Available: