In planta determination of GaMyb transcription factor as a target of pathogen induced microRNA, mir159

Date

2011-09-01

Author

Akkaya, Mahinur
Dagdas, Gulay Gok
Dagdas, Yasin F.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

204
views

0
downloads

One of the molecular mechanisms underlying the plant–pathogen interactions is the regulation of gene expressions in plant responses by microRNAs which are either stimulated or silenced against pathogen attacks. Among the plant miRNAs, we found that mir159 is one of which that differentially expressed upon Blumeria graminis f. sp. hordei (Bgh) infected resistant and susceptible barley lines. The study aims to confirm its role in regulating one of its putative target genes, GaMyb transcription factor, in vivo. Thus, both mir159 and GaMyb genes were co-infiltrated into tobacco as constructs of pEarlyGate100 and GFP expressing pEarlyGate103, respectively, via agrobacterium transient transformation. Our results suggest that mir159 indeed reduces the expression of GaMyb transcription factor by which, for the first time, confirm it being a biological target of mir159. To further confirm the induced expression level and the biological role of mir159 in susceptible barley by virulent Bgh pathogen infection, the differential level of disease development should be investigated in a susceptible line of barley after the over expression and/or the silencing of the gene of mir159.

Subject Keywords

Biotechnology, Bioengineering, Biomedical Engineering

URI

https://hdl.handle.net/11511/52189

DOI

https://doi.org/10.1016/j.copbio.2011.05.121

Collections

Graduate School of Natural and Applied Sciences, Conference / Seminar

Suggestions

OpenMETU
Core

Analyses of extracellular protein production in Bacillus subtilis - II: Responses of reaction network to oxygen transfer at transcriptional level KOCABAŞ, PINAR; ÇALIK GARCİA, GÜZİDE; Çalık, Pınar; Ozdamar, Tuncer H. (Elsevier BV, 2017-11-15) Oxygen transfer influences intracellular fluxes which are orchestrated by genome and its transcription in Bacillus subtilis throughout fermentation in recombinant human growth hormone (rhGH) production. Responses of B. subtilis reaction network to oxygen transfer were analysed at transcriptional level with determined transcriptome and calculated intracellular fluxes by the reconstructed genome scale model iBsu1144(rhGH) based on updated gene-enzyme-reaction data. iBsu1144(rhGH) employing 1067 reactions link...
Development and brain delivery of chitosan-PEG nanoparticles functionalized with the monoclonal antibody OX26 Aktas, Y; Yemisci, M; Andrieux, K; Gursoy, RN; Alonso, MJ; Fernandez-Megia, E; Novoa-Carballal, R; Quinoa, E; Riguera, R; Sargon, MF; Celik, HH; Demir, Ayhan Sıtkı; Hincal, AA; Dalkara, T; Capan, Y; Couvreur, P (American Chemical Society (ACS), 2005-11-01) The inhibition of the caspase-3 enzyme is reported to increase neuronal cell survival following cerebral ischemia. The peptide Z-DEVD-FMK is a specific caspase inhibitor, which significantly reduces vulnerability to the neuronal cell death. However, this molecule is unable to cross the blood-brain barrier (BBB) and to diffuse into the brain tissue. Thus, the development of an effective delivery system is needed to provide sufficient drug concentration into the brain to prevent cell death. Using the avidin (...
Regulatory effects of alanine-group amino acids on serine alkaline protease production by recombinant Bacillus licheniformis Çalık, Pınar; Ozdamar, TH (Wiley, 2003-04-01) Influences of the concentration and addition time of alanine-group amino acids, i.e. alanine, leucine and valine, on serine alkaline protease (SAP) synthesis were investigated by Bacillus licheniformis (DSM 1969) carrying pHV1431::subC in a defined medium to identify the amino acids creating intracellular reaction-rate limitation in SAP production. While the precursors of alanine-group amino acids, pyruvate and alanine, did not affect SAP production considerably within the range 0-15 mM, the addition of leu...
Covalent immobilization of chloroperoxidase onto magnetic beads: Catalytic properties and stability Bayramoglu, Guelay; Kiralp, Senem; Yilmaz, Meltem; Toppare, Levent Kamil; Arica, M. Yakup (Elsevier BV, 2008-02-15) Amino groups containing magnetic beads were used in covalent immobilization of the enzyme "chloroperoxidase (CPO)" which is one of a few enzymes that can catalyse the peroxide dependent oxidation of a wide spectrum of organic and inorganic compounds. The magnetic poly(glycidylmethacrylate-methylmethacrylate-etbyleneglycol dimethacrylate), magnetic p(GMA-MMA-EGDMA) beads were prepared via suspension polymerization in the presence of ferric ions. The magnetic beads were characterized with scanning electron mi...
Differential expression of genes possibly involved in incompatible interaction between barley and powdery mildew ERSOY, FİGEN; Ridout, Christopher J.; Akkaya, Mahinur (2011-09-01) The various domains of a plant disease resistance protein from wheat were found to be interacting with yeast proteins when screened via yeast two hybrid analyses. These genes are considered to play roles in disease resistance response. Thus, the expression levels in Mla3 mediated Powdery Mildew (Blumeria graminis f.sp. hordei, Bgh) disease resistance in barley were determined. The barley homologs of ARD1, CPR7, CSE1, GCN2 and SRP72, were partially cloned and sequenced. Their differential expression was conf...

Citation Formats

M. Akkaya, G. G. Dagdas, and Y. F. Dagdas, “In planta determination of GaMyb transcription factor as a target of pathogen induced microRNA, mir159,” 2011, vol. 22, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/52189.