Differential expression of genes possibly involved in incompatible interaction between barley and powdery mildew

Date

2011-09-01

Author

ERSOY, FİGEN
Ridout, Christopher J.
Akkaya, Mahinur

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The various domains of a plant disease resistance protein from wheat were found to be interacting with yeast proteins when screened via yeast two hybrid analyses. These genes are considered to play roles in disease resistance response. Thus, the expression levels in Mla3 mediated Powdery Mildew (Blumeria graminis f.sp. hordei, Bgh) disease resistance in barley were determined. The barley homologs of ARD1, CPR7, CSE1, GCN2 and SRP72, were partially cloned and sequenced. Their differential expression was confirmed using qRT-RCR at 6 hpi, 12 hpi, 24 hpi and 72 hpi upon incompatible Bgh infection in a resistant barley line (Pallas-02). All of the genes except HvCPR7 showed maximum expression levels at 12 hpi and gradually dropped at 24 and 72 hpi. On the other hand, HvCPR7 showed highest induction at 6 hpi, indicating that HvCRP7 is an even earlier responding gene. Most of the human homologues of these genes have been widely studied and found to have roles in apoptosis. As apoptosis is very important in the plant disease resistance response, qRT-PCR was performed to check if the genes are induced in resistant barley after powdery mildew inoculation. All the five genes were found to be differentially expressed in incompatible interactions between barley and powdery mildew.

Subject Keywords

Biotechnology, Bioengineering, Biomedical Engineering

URI

https://hdl.handle.net/11511/53398

DOI

https://doi.org/10.1016/j.copbio.2011.05.116

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Graduate School of Natural and Applied Sciences, Conference / Seminar

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Citation Formats

F. ERSOY, C. J. Ridout, and M. Akkaya, “Differential expression of genes possibly involved in incompatible interaction between barley and powdery mildew,” 2011, vol. 22, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53398.