Differential expression of genes possibly involved in incompatible interaction between barley and powdery mildew

Ridout, Christopher J.
Akkaya, Mahinur
The various domains of a plant disease resistance protein from wheat were found to be interacting with yeast proteins when screened via yeast two hybrid analyses. These genes are considered to play roles in disease resistance response. Thus, the expression levels in Mla3 mediated Powdery Mildew (Blumeria graminis f.sp. hordei, Bgh) disease resistance in barley were determined. The barley homologs of ARD1, CPR7, CSE1, GCN2 and SRP72, were partially cloned and sequenced. Their differential expression was confirmed using qRT-RCR at 6 hpi, 12 hpi, 24 hpi and 72 hpi upon incompatible Bgh infection in a resistant barley line (Pallas-02). All of the genes except HvCPR7 showed maximum expression levels at 12 hpi and gradually dropped at 24 and 72 hpi. On the other hand, HvCPR7 showed highest induction at 6 hpi, indicating that HvCRP7 is an even earlier responding gene. Most of the human homologues of these genes have been widely studied and found to have roles in apoptosis. As apoptosis is very important in the plant disease resistance response, qRT-PCR was performed to check if the genes are induced in resistant barley after powdery mildew inoculation. All the five genes were found to be differentially expressed in incompatible interactions between barley and powdery mildew.


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The metabolic fluxes through the central carbon pathways were calculated for the genus Bacillus separately for the enzymes serine alkaline protease (SAP), neutral protease (NP) and alpha -amylase (AMY) on five carbon sources that have different reduction degrees (gamma), to determine the theoretical ultimate limits of the production capacities of Bacillus species and to predict the selective substrate for the media design. Glucose (gamma = 4.0), acetate (gamma = 4.0), and the TCA cycle organic-acids succina...
QCM-based DNA biosensor for detection of genetically modified organisms (GMOs)
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Development of a mass sensitive quartz crystal microbalance (QCM)-based DNA biosensor for the detection of the hybridization of CaMV 35S promoter sequence (P35S) was investigated for the screening of genetically modified organisms (GMOs). Attention was focused on the choice of the coating chemistry that could be used for the immobilization of probe sequences on the gold surface of the quartz crystal. Two immobilization procedures were tested and compared considering the amount of the immobilized P35S probe ...
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Akkaya, Mahinur; Dagdas, Gulay Gok; Dagdas, Yasin F. (2011-09-01)
One of the molecular mechanisms underlying the plant–pathogen interactions is the regulation of gene expressions in plant responses by microRNAs which are either stimulated or silenced against pathogen attacks. Among the plant miRNAs, we found that mir159 is one of which that differentially expressed upon Blumeria graminis f. sp. hordei (Bgh) infected resistant and susceptible barley lines. The study aims to confirm its role in regulating one of its putative target genes, GaMyb transcription factor, in vivo...
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Çalık, Pınar; Ozdamar, TH (Wiley, 2003-04-01)
Influences of the concentration and addition time of alanine-group amino acids, i.e. alanine, leucine and valine, on serine alkaline protease (SAP) synthesis were investigated by Bacillus licheniformis (DSM 1969) carrying pHV1431::subC in a defined medium to identify the amino acids creating intracellular reaction-rate limitation in SAP production. While the precursors of alanine-group amino acids, pyruvate and alanine, did not affect SAP production considerably within the range 0-15 mM, the addition of leu...
Citation Formats
F. ERSOY, C. J. Ridout, and M. Akkaya, “Differential expression of genes possibly involved in incompatible interaction between barley and powdery mildew,” 2011, vol. 22, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53398.