Caglayani, Zeynep
Sel, Kaan
Yalcin, Yagmur Demircan
Sukas, Ozlem Sardan
Külah, Haluk
This study presents an approach for analyzing the dielectrophoretic (DEP) spectra of biological cells without ascertaining their membrane and cytoplasmic properties. DEP spectrum device with reciprocal V-shaped planar-electrodes was utilized to obtain DEP spectra information of cells. Integrating electric field simulations based on a strong theory with the experimental data enables determination of the DEP spectrum at a range of frequencies, for the first in the literature. The proposed method is tested with MCF7 breast cancer cells and DEP spectra are obtained for 15 different frequencies in the range from 100 kHz to 50 MHz at 10 V-pp.


Investigation of parameters affecting morphology of microfiltration and ultrafiltration membranes fabricated via phase separation microfabrication
Bolat, Cankut Kaan; Çulfaz Emecen, Pınar Zeynep; Bat, Erhan; Department of Chemical Engineering (2017)
This study examines the factors affecting the morphology of polyethersulfone membranes produced via phase separation microfabrication technique. Flat and corrugated membranes were prepared using a combination of vapor induced and liquid induced phase separation, and characterized using scanning electron microscopy, pure water permeance tests and Baker’s yeast (Saccharomyces Cerevisiae) fouling tests. An inverse asymmetric morphology was tried to be achieved for corrugated membranes, in order to increase the...
Exploring the relationship between cytoplasmic ion content variation and multidrug resistance in cancer cells via ion-release based impedance spectroscopy
Demircan Yalçın, Yağmur; Sukas, Sertan; Töral, Taylan Berkin; Gündüz, Ufuk; Külah, Haluk (2019-07-01)
This study aimed to measure and compare the cytoplasmic ion concentrations of the parental cells and their drug resistant progenies. High-level laboratory and clinically-relevant multidrug resistance (MDR) cell line models were developed and investigated for K562 and CCRF-CEM cells, respectively. Measurements were achieved using a modified version of ion release-based impedance spectroscopy (IRbIS), where the average total ion concentration per cell was determined by measuring the variations in solution con...
Characterization of extracellular beta-lactamases from penicillin G-resistant cells of Streptococcus thermophilus
Chirica, LC; Güray, Nülüfer Tülün; Gültekin, Güzin Candan; Bozoglu, F (International Association for Food Protection, 1998-07-01)
In this study, biochemical properties of two extracellular beta-lactamases produced by penicillin-resistant Streptococcus thermophilus cells were investigated. Both beta-lactamases showed specificity for penicillins but not for cephaloridins. The p-lactamases exhibited different affinities for penicillin G. The one with the higher molecular weight (F1) had a K(m) value of 3.44 mu M and a V(max), value of 8.33, mu mol/min/mg of protein, whereas the beta-lactamase with the lower molecular weight (FII) had a K...
Comparison of antioxidant enzyme activities and DNA damage in chickpea (Cicer arietinum L.) genotypes exposed to vanadium
Imtiaz, Muhammad; Mushtaq, Muhammad Adnan; Rizwan, Muhammad Shahid; Arif, Muhammad Saleem; Yousaf, Balal; Ashraf, Muhammad; Xiong Shuanglian, Xiong Shuanglian; Rizwan, Muhammad; Mehmood, Sajid; Tu, Shuxin (2016-10-01)
The present study was done to elucidate the effects of vanadium (V) on photosynthetic pigments, membrane damage, antioxidant enzymes, protein, and deoxyribonucleic acid (DNA) integrity in the following chickpea genotypes: C-44 (tolerant) and Balkasar (sensitive). Changes in these parameters were strikingly dependent on levels of V, at 60 and 120 mg V L-1 induced DNA damage in Balkasar only, while photosynthetic pigments and protein were decreased from 15 to 120 mg V L-1 and membrane was also damaged. It was...
Preparation of Chitosan-Coated Magnetite Nanoparticles and Application for Immobilization of Laccase
Kalkan, Nuzhet Ayca; AKSOY, SERPİL; Aksoy, Eda Ayse; Hasırcı, Nesrin (2012-01-15)
In this study, immobilization of laccase (L) enzyme on magnetite (Fe(3)O(4)) nanoparticles was achieved, so that the immobilized enzyme could be used repeatedly. For this purpose, Fe(3)O(4) nanoparticles were coated and functionalized with chitosan (CS) and laccase from Trametes versicolor was immobilized onto chitosan-coated magnetic nanoparticles (Fe(3)O(4)-CS) by adsorption or covalent binding after activating the hydroxyl groups of chitosan with carbodiimide (EDAC) or cyanuric chloride (CC). For chitosa...
Citation Formats
Z. Caglayani, K. Sel, Y. D. Yalcin, O. S. Sukas, and H. Külah, “ANALYSIS OF THE DIELECTROPHORETIC (DEP) SPECTRA OF BIOLOGICAL CELLS,” 2017, Accessed: 00, 2020. [Online]. Available: