Biochemical characterization of Thermoplasma volcanium recombinant 20S proteasome and its regulatory subunit



Biochemical characterization of recombinant 20s proteasome from thermoplasma volcanium and cloning of it's regulatory subunit gene
Baydar, Gözde; Kocabıyık, Semra; Department of Biotechnology (2006)
In this study, we have characterized some biochemical and electrophoretic features of recombinant 20S Proteasome from a thermoacidophilic archaeon Thermoplasma volcanium. As revealed by SDS-PAGE the 20S Proteasome was composed of two subunits, ?- and β- subunits with estimated molecular masses of 24 kDa and 23 kDa, respectively. The highest chymotryptic activity was observed over an alkaline pH range (pH 8.0 ا pH 9.0) and the optimum temperature for the activity was determined as 85oC. The heat stability of...
Biochemical characterization of R.oryzae xylanase and heterologous probe preparation from T. reesei
Ersayın, Ayşegül; Bakır, Ufuk; Türkel, Sezai; Department of Biotechnology (2000)
AFKHAMI, P; HALICIGIL, C; Özcengiz, Gülay; ALAEDDINOGLU, NG (1993-06-17)
Biochemical and immunological characterization of beef liver NADPH-cytochrome P450 reductase
Çelik, Haydar; Arınç, Emel; Department of Biochemistry (2002)
Biochemical and Molecular Characterization of Strontium-resistant Environmental Isolates of Pseudomonas fluorescens and Sphingomonas paucimobilis
Ozer, Gamze; Ergene, Aysun; İçgen, Bülent (Informa UK Limited, 2013-03-13)
Strontium resistance was investigated in strains isolated from the river Kzlrmak which has been affected by industrial and agricultural heavy metal pollution from the surrounding facilities and domestic effluents from the city Krkkale, Turkey. Strontium-resistant isolates with a minimal inhibitory concentration of 2000mg L-1 were isolated and identified as Pseudomonas fluorescens and Sphingomonas paucimobilis. Both isolates were shown to be resistant to other heavy metals like aluminum, lithium, barium, nic...
Citation Formats
G. Baydar and S. Kocabıyık, “Biochemical characterization of Thermoplasma volcanium recombinant 20S proteasome and its regulatory subunit,” 2005, vol. 272, Accessed: 00, 2020. [Online]. Available: