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Metabolic engineering of L-phenylalanine pathway in Bacillus subtilis
Date
2005-08-01
Author
Demirci, Y
Çalık, Pınar
Calik, G
Ozdamar, TH
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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https://hdl.handle.net/11511/53614
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Department of Chemical Engineering, Conference / Seminar
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Metabolic control sites in the aromatic group amino acid pathway (AAAP) of Bacillus subtilis for L-Phenylalanine (Phe) overproduction were determined; and aiming pathway flux amplification, by cloning the flux controlling gene aroH to a multi-copy plasmid, the impact of single gene cloning on pathway flux distributions were investigated. The branch-point metabolites E4P and PEP+E4P supplied in vitro, enhanced Phe production and well defined perturbations were achieved on the AAAP reactions. The intracellula...
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Y. Demirci, P. Çalık, G. Calik, and T. Ozdamar, “Metabolic engineering of L-phenylalanine pathway in Bacillus subtilis,” 2005, vol. 118, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53614.