Metabolic engineering of L-phenylalanine pathway in Bacillus subtilis

Demirci, Y
Çalık, Pınar
Calik, G
Ozdamar, TH


Metabolic engineering of aromatic group amino acid pathway in Bacillus subtilis for L-phenylalanine production
Ozcelik, IS; Çalık, Pınar; Calik, G; Ozdamar, TH (Elsevier BV, 2004-11-01)
Metabolic control sites in the aromatic group amino acid pathway (AAAP) of Bacillus subtilis for L-Phenylalanine (Phe) overproduction were determined; and aiming pathway flux amplification, by cloning the flux controlling gene aroH to a multi-copy plasmid, the impact of single gene cloning on pathway flux distributions were investigated. The branch-point metabolites E4P and PEP+E4P supplied in vitro, enhanced Phe production and well defined perturbations were achieved on the AAAP reactions. The intracellula...
Metabolic reaction network of Pichia pastoris with glycosylation reactions: Flux analysis for erythropoietin production
Eskitoros, Melda S.; Ata, Ozge; Çalık, Pınar (Wiley, 2014-11-01)
BACKGROUND: Biochemical reaction network of Pichia pastoris was improved by including N-glycosylation pathway reactions to determine the intracellular reaction rates for glycosylated protein production.
Metabolic engineering of aspartate pathway for increased production of cephamycin C in Streptomyces clavuligerus
Özcengiz, Gülay (null; 2009-09-27)
Metabolic engineering with a novel promoter in Pichia pastoris for recombinant human growth hormone production: effects of oxygen transfer conditions
Kalender, Özge; Çalık, Pınar; Özdamar, Tunçer; Department of Chemical Engineering (2018)
The objectives of this thesis are investigation of, i) the effects of oxygen transfer conditions on recombinant protein production in Pichia pastoris strains designed with novel naturally occuring pyruvate kinase (PYK) promoter (PPYK) which is a potential promoter for recombinant protein production under low to moderate oxygen transfer conditions, and ii) influences of engineering with single- and multi- copy genes, in fed-batch fermentation processes. Production of recombinant human growth hormone (rhGH) b...
Metabolic changes in schizophrenia and human brain evolution
Khaitovich, Philipp; Lockstone, Helen E.; Wayland, Matthew T.; Tsang, Tsz M.; Jayatilaka, Samantha D.; Guo, Arfu J.; Zhou, Jie; Somel, Mehmet; Harris, Laura W.; Holmes, Elaine; Paeaebo, Svante; Bahn, Sabine (2008-01-01)
Background: Despite decades of research, the molecular changes responsible for the evolution of human cognitive abilities remain unknown. Comparative evolutionary studies provide detailed information about DNA sequence and mRNA expression differences between humans and other primates but, in the absence of other information, it has proved very difficult to identify molecular pathways relevant to human cognition.
Citation Formats
Y. Demirci, P. Çalık, G. Calik, and T. Ozdamar, “Metabolic engineering of L-phenylalanine pathway in Bacillus subtilis,” 2005, vol. 118, Accessed: 00, 2020. [Online]. Available: