An automated blood cell analysis and classification system

Direct analysis of blood and bone marrow smear images obtained from microscope are not common in current trends of hematology. Current blood smear analysis methods heavily depend on flow cytometry based techniques in which the blood cells are flew through microtubes thus classified according to their flow characteristics and cell volumes. This method is an indirect way of measuring features hence is accurate at a certain level. In this work an automated blood cell classification system is presented including cell segmentation, cell partitioning and classification.


A 3-D vascular connectivity tracking and vascular network extraction toolkit
Kara, Kerim; Eyüboğlu, Behçet Murat; Çekirge, H. Saruhan; Department of Biomedical Engineering (2011)
Angiography is an invasive procedure since contrast medium is injected into circulatory system of patients and the mostly preferred technique is X-ray angiography. For diagnosis, treatment planning, and risk assessment purposes, interventional radiologists utilize visual inspection to determine connectivity relations between vessels. This situation leads angiography to be more invasive, since it requires additional injection of contrast medium and X-ray dose. This thesis work presents a 3-D vascular connect...
Contact guidance enhances the quality of a tissue engineered corneal stroma
Vrana, E.; Builles, N.; Hindie, M.; Damour, O.; AYDINLI, ATİLLA; Hasırcı, Vasıf Nejat (2008-02-01)
Corneal stroma is a very complex structure, composed of 200 lamellae of oriented collagen fibers. This highly complex nature of cornea is known to be important for its transparency and mechanical integrity. Thus, an artificial cornea design has to take into account this complex structure. In this study, behavior of human corneal keratocytes on collagen films patterned with parallel channels was investigated. Keratocytes proliferated well on films and reached confluency after 7 days in the incubation medium....
Aydemir, Umut; Erel Göktepe, İrem; Akdağ, Akın; Department of Biochemistry (2021-9-24)
Boron Neutron Capture Therapy (BNCT) is a non-invasive radiotherapy technique which is based on delivery of non-radioactive 10B compounds to cancer cells and irradiation of the tumour tissues with low energy thermal neutrons. It is a promising radiotherapy technique due to reduce the side effects of the treatment. The main goal of this thesis is to prepare a non-toxic polymer vehicle to provide efficient delivery of 10B to cancer cells. Dodecahydro-closo-dodecaborate (B12H12)2- (B12) anion was utilized as a...
A DEP-Based Lab-On-A-Chip System For The Detection Of Multidrug Resistance In K562 Leukemia Cells
Yalçın, Yağmur Demircan; Özkayar, Gürkan; Özgür, Ebru; Gündüz, Ufuk; Külah, Haluk (2016-06-09)
This study presents a DEP-based lab-on-a-chip (LOC) system for label-free detection of multidrug resistant (MDR) K562 leukemia cells in a cell mixture, consisting of red blood cells (RBCs) and MDR-K562 cells, for the first time in the literature. The system consists of 2 consecutive DEP units, one for the depletion of RBCs and the other for capturing of MDR-K562 cells. RBCs are depleted by 60% in the first unit. In the second unit, MDR-K562 cell detection is performed with 100% selectivity at a flow rate of...
Examination of the dielectrophoretic spectra of MCF7 breast cancer cells and leukocytes
Çağlayan, Zeynep; Demircan Yalçın, Yağmur; Külah, Haluk (Wiley, 2020-03-01)
The detection of circulating tumor cells (CTCs) in blood is crucial to assess metastatic progression and to guide therapy. Dielectrophoresis (DEP) is a powerful cell surface marker-free method that allows intrinsic dielectric properties of suspended cells to be exploited for CTC enrichment/isolation from blood. Design of a successful DEP-based CTC enrichment/isolation system requires that the DEP response of the targeted particles should accurately be known. This paper presents a DEP spectrum method to inve...
Citation Formats
G. Ongun et al., “An automated blood cell analysis and classification system,” 1998, vol. 53, Accessed: 00, 2020. [Online]. Available: