Effect of arachidonic acid on specific binding of [H-3]naloxone to opioid receptors

The effects of arachidonic acid (AA) on binding of [H-3]naloxone to the agonist and antagonist configurations of opioid receptors were investigated in rat brain. Equilibrium binding parameters of the agonist and antagonist configurations of the receptors were evaluated from homologue displacement data in the presence or absence of AA. Addition of AA at a concentration of 0.6 mM (1.5 mu mole/mg of protein) reduced by 22% and 53% the maximal number of binding sites (B-max) respectively in the absence or presence of 100 mM NaCl. Binding affinity (K-D) was not altered significantly (P<0.05) either in the presence or absence of 100 mM NaCl and AA. We conclude that AA mediated reduction in [H-3]naloxone specific binding was chiefly due to a decrease in the number of binding sites.


Effect of lipids on binding characteristics of opiod receptors
Apaydın, Serpil; Öktem, Hüseyin Avni; Department of Biochemistry (2005)
Effect of lipids on binding characteristics of opioid receptors in membranes prepared from rat brain were studied. Lipid concentrations causing changes in specific binding of [3H]Endomorphin-1 (ProE1), an opioid agonist highly specific to mu-type opioid, [3H]Ile5,6deltorphin II (DIDI), an agonist ligand highly specific to delta type receptor and [3H]Naloxone (Nlx), a universal opioid receptor antagonist were determined. Inhibition of [3H]ProE1, [3H]DIDI and [3H]Nlx specific binding was also examined by homo...
Effects of chronic diazepam treatment and withdrawal on avoidance behavior
Ayvasik, B; Zagli, U; Palaoglu, O; Soygur, H; Ayhan, IH (1999-06-01)
The present study was designed to evaluate the effects of chronic diazepam (DZ) treatment with low doses and diazepam withdrawal on the two-way shuttle-box avoidance performance in rats. After 24 rats were randomly assigned into three groups (I mg/kg DZ, 2 mg/kg DZ and control groups), all animals were trained to avoid from an electrical foot shock (0.8 mA) delivered through the grid floor by crossing to the other compartment of the box. Following the avoidance training, the first group received I mg/kg DZ ...
Effects of risperidone treatment on the expression of hypothalamic neuropeptide in appetite regulation in Wistar rats
Kursungoz, Canan; AK, MEHMET; Yanık, Tülin (2015-01-30)
Although the use of atypical antipsychotic drugs has been successful in the treatment of schizophrenia, they can cause some complications in the long-term use, including weight gain. Patients using these drugs tend to disrupt treatment primarily due to side effects. The atypical antipsychotic mechanism of action regulates a number of highly disrupted neurotransmitter pathways in the brains of psychotic patients but may also cause impairment of neurohorrnonal pathways in different brain areas. In this study,...
Effects of 2-arylbenzimidazoles on rat hepatic microsomal monooxygenase system
İşcan, Mesude Y.; Büyükbingöl, Erdem; İşcan, Mümtaz; Şahin, Fethi; Şafak, Cihat (Elsevier BV, 1989-1)
1. The effects of eight newly synthesized 2-aryl substituted benzimidazole derivatives on control and phenobarbital (PB) treated rat liver microsomal aniline 4-hydroxylase and ethylmorphine N-demethylase activities, and their binding to control and PB-treated rat liver microsomal oxidized cytochrome P-450 are presented. 2. All compounds inhibited ethylmorphine N-demethylase activity with I50 values ranging from 8.50 × 10−4 M to 27.83 × 10−4 M in control and ranging from 2.80 × 10−4 M to 15.79 × 10−4 M in P...
Effect of neuropeptide-y (NPY) on bone metabolism as a neuromediator
Çevik, Muammer Özgür; Korkusuz, Feza; Department of Biotechnology (2004)
In order to elucidate the effects of NPY directly on bone tissue, two different doses of NPY (NPY dose 1= 1X10-5 M and NPY dose 2 = 1X 10?6 M) and NPY dose 2 plus its inhibitor were applied together with hyaluronic acid (HA) into the intramedullary area of right tibia of Wistar rats. HA alone was administered as the control group. On three time points, day one, week one and week two after administration, the tibiae were collected and stored at ?20oC for analysis. Evaluation was performed via conventional ra...
Citation Formats
S. Apaydin and H. A. Öktem, “Effect of arachidonic acid on specific binding of [H-3]naloxone to opioid receptors,” ACTA NEUROBIOLOGIAE EXPERIMENTALIS, pp. 361–363, 2000, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55012.