MOLECULAR PHYLOGENY OF TRITICUM AND AEGILOPS GENERA BASED ON ITS AND MATK SEQUENCE DATA

2016-02-01
Dizkırıcı, Ayten
Kansu, Çiğdem
Önde, Sertaç
Understanding the phylogenetic relationship between Triticum and Aegilops species, which form a vast gene pool of wheat, is very important for breeding new cultivated wheat varieties. In the present study, phylogenetic relationships between Triticum (12 samples from 4 species) and Aegilops (24 samples from 8 species) were investigated using sequences of the nuclear ITS rDNA gene and partial sequences of the matK gene of chloroplast genome. The phylogenetic relationships among species were reconstructed using Maximum Likelihood method. The constructed tree based on the sequences of the nuclear component (ITS) displayed a close relationship between polyploid wheats and Aegilops speltoides species which provided new evidence for the source of the enigmatic B genome donor as Ae. speltoides. Concurrent clustering of Ae. cylindrica and Ae. tauschii and their close positioning to polyploid wheats pointed the source of the D genome as one of these species. As reported before, diploid Triticum species (i.e. T. urartu) were identified as the A genome donors and the positioning of these diploid wheats on the constructed tree are meaningful. The constructed tree based on the chloroplastic matK sequences displayed same relationship between polyploid wheats and Ae. speltoides species providing evidence for the later species being the chloroplast donors for polyploid wheats. Therefore, our results supported the idea of coinheritance of nuclear and chloroplast genomes where Ae. speltoides was the maternal donor. For both trees the remaining Aegilops species produced a distinct cluster whereas with the exception of T. urartu, diploid Triticum species displayed a monophyletic structure.
PAKISTAN JOURNAL OF BOTANY

Suggestions

Molecular phylogeny of relict-endemic Liquidambar orientalis Mill based on sequence diversity of the chloroplast-encoded matK gene
Ozdilek, Asli; Cengel, Burcu; Kandemir, Gaye; Tayanc, Yasemin; Velioglu, Ercan; Kaya, Zeki (2012-02-01)
The genetic diversity and evolutionary divergence in Liquidambar species and Liquidambar orientalis varieties were compared with respect to the matK gene. A total of 66 genotypes from 18 different populations were sampled in southwestern Turkey. The matK region, which is about 1,512 bp in length, was sequenced and studied. L. orientalis, L. styraciflua, and L. formosana had similar magnitude of nucleotide diversity, while L. styraciflua and L. acalycina possessed higher evolutionary divergence. The highest ...
Genetic diversity and relationship analysis among accessions of Aegilops ssp in Turkey using amplified fragment length polymorphism (AFLP) markers
Kaya, İlhan; Kirisozu, Asude Callak; Ersoy, Figen Yildirim; Dere, Sahin; Akkaya, Mahinur (2011-11-16)
Amplified fragment length polymorphism (AFLP) DNA markers were used to assess the genetic diversity and relationships between 55 accessions of genus Aegilops, including the species Aegilops triuncialis L. (UUCC), Aegilops geniculata Roth (MMUU), Aegilops cylindrica Host (CCDD) and Aegilops umbellulata Zhuk (UU). The samples were collected from Aegean region and East Anatolia, Turkey. 16 AFLP selective primer combinations generated a total of 3200 polymorphic amplification products. 50 Aegilops accessions we...
Molecular phylogenetic analysis resolving the taxonomic discrepancies among Salix L. species naturally found in Turkey
Acar, Pelin; Değirmenci, Funda Ö.; DUMAN, HAYRİ; Kaya, Zeki (2022-01-01)
Detailed phylogenetic relationships and molecular dating are still quite rare for the complex and diverse genus Salix L. Here we focus on the taxonomic status and phylogeny of twenty-six Salix taxa naturally found in Turkey using the chloroplast DNA regions (trn T-F, matK, and rbcL) to unravel the relationship among them. The status of Salix species was also checked in the phylogenetic tree constructed with the data from Internal Transcribed Spacer (ITS) region of nuclear gene, including 158 accessions from...
Molecular phylogenetic analyses of Juniperus L. species in Turkey and their relations with other junipers based on cpDNA
Güvendiren, Aysun Demet; Kaya, Zeki; Department of Biology (2015)
Evolutionary relationships within and among two sections and three subsections of Juniperus species (Section Juniperus, subsections Juniperus, Oxycedrus and Caryocedrus, Section Sabina) naturally distributed in Turkey were investigated with molecular variations of chloroplast DNA (cpDNA). This study revealed the phylogenetic relation of 66 individuals from 7 native Turkish Juniperus L. species based upon DNA sequence of trnL intron (trnL5’-L3’) , trnL3’- F(GAA) (trnL-F intergenic spacer), trnV intron and ma...
Phylogenetic relationships of Elymus L. and related genera (Poaceae) based on the nuclear ribosomal internal transcribed spacer sequences
Dizkirici, Ayten; KAYA, ZEKİ; Cabi, Evren; Doğan, Musa (2010-01-01)
To help in the clarification of the taxonomic status of Elymus L and related genera Agropyron Gaertn Leymus Hochst Hordelymus (Jess) Harz and Brachypodium P Beauv, the sequence diversity in the internal transcribed spacer (ITS) region of nuclear ribosomal DNA was studied ITS data of 64 Triticeae accessions including 40 Elymus 12 Agropyron 7 Leymus and 4 Hordelymus and 1 Brachypodium as an out group were analysed The molecular diversity statistics Indicated that the most diverse genus is Elymus among the stu...
Citation Formats
A. Dizkırıcı, Ç. Kansu, and S. Önde, “MOLECULAR PHYLOGENY OF TRITICUM AND AEGILOPS GENERA BASED ON ITS AND MATK SEQUENCE DATA,” PAKISTAN JOURNAL OF BOTANY, pp. 143–153, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55543.