Dye derived and metal incorporated affinity poly(2-hydroxyethyl methacrylate) membranes for use in enzyme immobilization

Date

1998-08-01

Author

Arica, MY
Denizli, A
Baran, T
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

139
views

0
downloads

Microporous poly(2-hydroxyethyl methacrylate) (PHEMA) membranes were prepared by W-initiated photopolymerization of HEMA in the presence of an initiator (alpha,alpha'-azobisisobutyronitrile, AIBN). An affinity dye Cibacron Blue F3GA (CB) was attached covalently and then Fe3+ ions incorporated. The PHEMA-CB and PHEMA-CB-Fe3+ membranes derived were used for adsorption of glucose oxidase (GOD). The adsorption capacities of these membranes were determined under conditions of different pH and with different concentrations of the adsorbate in the medium. The adsorption phenomena appeared to follow a typical Langmuir isotherm. The glucose oxidase adsorption capacity of the Fe3+ incorporated membrane (87 mu g cm(-2)) was greater than that of the dye-derived membrane (66 mu g cm(-2)). Non-specific adsorption of the glucose oxidase on the PHEMA membranes was negligible. The K-m values for both immobilized glucose oxidase PHEMA-CB-GOD (8.3) and PHEMA-CB-Fe3+-GOD (7.6) were higher than that of the free enzyme (6.2 mM). Optimum reaction pH was 5.5 for the free and 6.0 for both immobilized preparations. The optimum reaction temperature of the adsorbed enzymes was 5 degrees C higher than that of the free enzyme and was significantly broader. After 15 successive uses the retained activity of the adsorbed enzyme was 87%. It was observed that enzymes could be repeatedly adsorbed and desorbed on the derived PHEMA membranes without significant loss in adsorption capacity or enzymic activity. (C) 1998 SCI.

Subject Keywords

Glucose oxidase, Dye affinity membrane, Cibacron blue F3GA, PHEMA, Immobilization, Adsorption, Metal chelate affinity membrane

URI

https://hdl.handle.net/11511/56174

Journal

POLYMER INTERNATIONAL

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Catalase adsorption onto cibacron blue F3GA and Fe(III)derivatized poly(hydroxyethyl methacrylate) membranes and application to a continuous system Arica, MY; Denizli, A; Salih, B; Piskin, E; Hasırcı, Vasıf Nejat (1997-06-25) Poly(2-hydroxyethyl methacrylate) (poly(HEMA)) membranes were prepared by W-initiated photopolymerization of HEMA in the presence of an initiator (a-a'-azobisisobutyronitrile, AIBN). An affinity dye, i.e. Cibacron Blue F3GA (CB) was incorporated covalently and then complexed with Fe(III) ions. The polyHEMA-CB and polyHEMA-CB-Fe(III) derivatized membranes were used in the adsorption of catalase (CAT). The enzyme-loading capability of the Fe(III)-containing membrane (23.6 mu g/cm(2)) was greater than that of ...
Cibacron Blue F3GA-incorporated macroporous poly(2-hydroxyethyl methacrylate) affinity membranes for heavy metal removal Denizli, A; Salih, B; Arica, MY; Kesenci, K; Hasırcı, Vasıf Nejat; Piskin, E (1997-01-17) Macroporous poly(2-hydroxyethyl methacrylate), poly(HEMA), membranes were prepared by UV-initiated photo-polymerization of HEMA in the presence of an initiator (azobisisobutyronitrile, AIBN). An affinity dye, i.e., Cibacron Blue F3GA was then incorporated covalently. These affinity membranes with a swelling ratio of 58%, and carrying 10.67 mmol Cibacron Blue F3GA/m(2) membrane were used in the adsorption/desorption of some selected heavy metal ions [i.e., As(III), Cd(II) and Pb(II)] from aqueous media. Very...
Comparison of beta-galactosidase immobilization by entrapment in and adsorption on poly(2-hydroxyethylmethacrylate) membranes Baran, T; Arica, MY; Denizli, A; Hasırcı, Vasıf Nejat (1997-12-01) beta-Galactosidase was immobilized in/on poly(2-hydroxyethyl methacrylate) (pHEMA) membranes by two different methods: adsorption on Cibacron F3GA derivatized pHEMA membranes (pHEMA-CB), and entrapment in the bulk of the pHEMA membranes. The maximum beta-galactosidase adsorption on pHEMA-CB membranes was obtained as 95.6 mu g cm(-2) in 2.0 mg cm(-3) enzyme solution. The adsorption phenomena appeared to follow a typical Langmuir isotherm. In the entrapment, an increase in beta-galactosidase loading resulted ...
COVALENT IMMOBILIZATION OF ASPERGILLUS-NIGER ON PHEMA MEMBRANE - APPLICATION TO CONTINUOUS-FLOW REACTORS ARICA, MY; SHARIF, FA; ALAEDDINOGLU, NG; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (1993-01-01) Poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was prepared via photopolymerization and activated with epichlorohydrin. The conidia of Aspergillus niger strains (wild type 'NRRL-3' and genetically improved strain 'NRRL-3/2-2A') were covalently-immobilized on the membranes. Uniform growth of A. niger cells on membrane surfaces was verified by SEM. The glucose oxidase (GOD) activity of the immobilized cells was determined in a continuous flow membrane reactor (CFMR) by assaying for hydrogen peroxide produ...
GLUCOSE-OXIDASE SANDWICHED BETWEEN PHEMA LAYERS - A CONTINUOUS-FLOW REACTOR APPLICATION ARICA, MY; Hasırcı, Vasıf Nejat (1993-09-01) Glucose oxidase was entrapped between poly(2-hydroxyethyl methacrylate) membranes and conditions were optimized for high enzyme activity and high levels of entrapment. Highest entrapment was with a 78 mum thick coat. A continuous flow membrane reactor was designed and used. The reaction was first order with respect to glucose and to oxygen. V(max) values for the native and immobilized enzymes were 0.182 and 0.133 mm/min. The K(m)'s for native and immobilized enzymes were 6.2 and 16.9 mm, respectively. At hi...

Citation Formats

M. Arica, A. Denizli, T. Baran, and V. N. Hasırcı, “Dye derived and metal incorporated affinity poly(2-hydroxyethyl methacrylate) membranes for use in enzyme immobilization,” POLYMER INTERNATIONAL, pp. 345–352, 1998, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/56174.