Catalase adsorption onto cibacron blue F3GA and Fe(III)derivatized poly(hydroxyethyl methacrylate) membranes and application to a continuous system

Date

1997-06-25

Author

Arica, MY
Denizli, A
Salih, B
Piskin, E
Hasırcı, Vasıf Nejat

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Poly(2-hydroxyethyl methacrylate) (poly(HEMA)) membranes were prepared by W-initiated photopolymerization of HEMA in the presence of an initiator (a-a'-azobisisobutyronitrile, AIBN). An affinity dye, i.e. Cibacron Blue F3GA (CB) was incorporated covalently and then complexed with Fe(III) ions. The polyHEMA-CB and polyHEMA-CB-Fe(III) derivatized membranes were used in the adsorption of catalase (CAT). The enzyme-loading capability of the Fe(III)-containing membrane (23.6 mu g/cm(2)) was greater than that of the poly(HEMA)-CB derivatized membrane (17.1 mu g/cm(2)). The adsorption phenomena appeared to follow a typical Langmuir isotherm. The K-m values for both immobilized catalases (poly(HEMA)-CB-CAT (22.4 mM) and poly(HEMA)-CB-Fe(III)-CAT (19.3 mM)) were higher than that of free enzyme (16.5 mM). Optimum operational temperature was 5 degrees C higher than that of the free enzyme and was significantly broader. A similar observation was made for the optimum pH. Operational, thermal and storage stabilities were found to increase with immobilization, especially in the presence of Fe(III). It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss in adsorption capacity or enzyme activity.

Subject Keywords

Affinity membranes, Microporous, Porous membranes, Biological membranes, Enzyme immobilization, Dye affinity

URI

https://hdl.handle.net/11511/32178

Journal

JOURNAL OF MEMBRANE SCIENCE

DOI

https://doi.org/10.1016/s0376-7388(96)00334-1

Collections

Graduate School of Natural and Applied Sciences, Article

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PERMEABILITY OF PHEMA MEMBRANES PREPARED BY PHOTOINITIATION ARICA, MY; Hasırcı, Vasıf Nejat (1993-01-01) Poly(2-hydroxyethyl methacrylate) membranes were prepared in aqueous media with different ions and ionic strengths. The enzyme glucose oxidase was incorporated into some of these membranes. It was found that membrane water contents and ionic strengths have a linear relationship as long as the salt is not changed. When the salt was varied, only a relationship between salt concentration and water content could be observed. The membrane permeabilities were all found to be of the same order (10(-8) cm2 s-1) but...

Citation Formats

M. Arica, A. Denizli, B. Salih, E. Piskin, and V. N. Hasırcı, “Catalase adsorption onto cibacron blue F3GA and Fe(III)derivatized poly(hydroxyethyl methacrylate) membranes and application to a continuous system,” JOURNAL OF MEMBRANE SCIENCE, pp. 65–76, 1997, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32178.