Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Regulation and role of CCAAT/enhancer binding protein during differentiation of intestinal epithelial cells
Date
2013-09-01
Author
Memişoğlu, Aslı Sade
Banerjee, Sreeparna
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
229
views
0
downloads
Cite This
Background: The molecular mechanisms of differentiation of intestinal epithelial cells, is poorly understood and disruption of this balance may result in neoplastic transformation and malignant growth. The family of CCAAT/Enhancer Binding Protein (C/EBP) transcription factors is implicated in cellular growth, differentiation, inflammation and development and control differentiation in several cell types. The involvement of these transcription factors in intestinal differentiation is not known. The aim of this study is to elucidate the transcriptional, translational and post-translational regulation of C/EBP proteins and their role during the course of intestinal epithelial differentiation. Materials and Methods: The colorectal cancer cell line Caco-2, a well established differentiation model, was used. The expression of C/EBP genes was analyzed using publicly available microarray data. The protein levels and the phosphorylation status of translation initiation factors were checked by Western blot. The activity of C/EBP was determined by luciferase reporter and chromatin immunoprecipitation assays. Cytoplasmic calcium levels were determined fluorometrically by calcium binding with Fura-2AM. Results: Microarray data analyses of Caco-2 differentiation have shown that C/EBPa and b expression increases during differentiation. The major regulatory mechanism for C/EBP proteins is translational in which several isoforms are produced from alternative translation initiation sites. The ratio of the two isoforms C/EBPb1 to C/EBPb3 was found to be decreasing in differentiated Caco-2 cells. This change could be explained by the increase in the active translation initiation factor eIF2a. The activity of C/EBP was shown to increase together with an upregulation of its target genes. The post-translational regulation was investigated by determining SERCA-3 (less affinity to calcium) levels and was shown to decrease. This lead to an increase in the cytoplasmic calcium levels which in turn may cause activation of calcium dependent calmodulin kinase II, hence increase in C/EBP activity. Conclusions: In this study the involvement of C/EBP proteins in intestinal epithelial differentiation was investigated for the first time. We believe that the findings of this research would reveal important mechanisms underlying the intestinal differentiation-dedifferentiation processes and provide some candidate therapeutic targets for the prevention and treatment of colorectal cancer.
URI
https://hdl.handle.net/11511/56182
Collections
Department of Biology, Conference / Seminar
Suggestions
OpenMETU
Core
Investigation of the molecular pathways involved in intestinal epithelial differentiation
Sade Memişoğlu, Aslı; Banerjee, Sreeparna; Department of Biology (2014)
The molecular mechanisms of balanced and continuous generation of intestinal epithelial cells, is poorly understood and disruption of this balance may result in neoplastic transformation and malignant growth. Differentiation is regulated by numerous signals, which in turn regulate signaling pathways directing activation or inactivation of certain transcription factors. Perturbations like changes in Ca2+ levels, glucose or amino acid starvation result in an ER stress response, which is also implicated in the...
Activation of C/EBPbeta-3 during cellular differentiation links the development of ER stress and autophagy in colon epithelial cells
Memişoğlu, Aslı Sade; Tuncer, S.; Banerjee, Sreeparna (2016-09-01)
The molecular mechanisms of balanced and continuous generation of intestinal epithelial cells, is closely regulated and disruption of this balance may result in neoplastic transformation and malignant growth. Differentiation is regulated by numerous signals, which in turn regulate signaling pathways directing activation or inactivation of certain transcription factors. Perturbations like changes in Ca2+levels, glucose or amino acid starvation may result in an ER stress response, which is also implicated in ...
Alterations of ceramide/sphingosine 1-phosphate rheostat involved in the regulation of resistance to imatinib-induced apoptosis in K562 human chronic myeloid leukemia cells
Baran, Yusuf; Salas, Arelis; Senkal, Can E.; Gündüz, Ufuk; Bielawski, Jacek; Obeid, Lina M.; Ogretmen, Besim (2007-04-13)
In this study, mechanisms of resistance to imatinib-induced apoptosis in human K562 cells were examined. Continuous exposure to stepwise increasing concentrations of imatinib resulted in the selection of K562/IMA-0.2 and -1 cells, which expressed similar to 2.3- and 19-fold resistance, respectively. Measurement of endogenous ceramides by high performance liquid chromatography/mass spectroscopy showed that treatment with imatinib increased the generation of ceramide, mainly C-18-ceramide, which is generated ...
DynaDom: structure-based prediction of T cell receptor inter-domain and T cell receptor-peptide-MHC (class I) association angles
Hoffmann, Thomas; Marıon, Antoıne; Antes, Iris (Springer Science and Business Media LLC, 2017-02-02)
Background: T cell receptor (TCR) molecules are involved in the adaptive immune response as they distinguish between self- and foreign-peptides, presented in major histocompatibility complex molecules (pMHC). Former studies showed that the association angles of the TCR variable domains (Va/V beta) can differ significantly and change upon binding to the pMHC complex. These changes can be described as a rotation of the domains around a general Center of Rotation, characterized by the interaction of two highly...
Differential expressions and functions of phosphodiesterase enzymes in different regions of the rat heart
DERİCİ, MEHMET KÜRŞAT; SADİ, GÖKHAN; Cenik, Basar; Güray, Nülüfer Tülün; DEMİREL YILMAZ, EMİNE (Elsevier BV, 2019-02-05)
Phosphodiesterase enzymes (PDEs) are responsible for the adjustment of cyclic nucleotide levels. Alterations in PDE expressions in different tissues cause conflicts between functional and clinical effects of PDE inhibitors. Therefore, the aim of this study was to investigate the gene and protein expressions and the functional role of PDEs in atrium and ventricle of rat heart The expressions of PDEs were examined in cardiac intact tissues and enzymatically isolated cells. The effects of PDE1-5 inhibitors (vi...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
A. S. Memişoğlu and S. Banerjee, “Regulation and role of CCAAT/enhancer binding protein during differentiation of intestinal epithelial cells,” 2013, vol. 49, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/56182.
