From RFLP to DArT: molecular tools for wheat (Triticum spp.) diversity analysis

Khan, Mohd Kamran
Pandey, Anamika
Choudhary, Saumya
Akkaya, Mahinur
Thomas, George
Wheat (Triticum spp.) is a universally lucrative agricultural crop. An increase in wheat production has been shown through selection by the farmers which can increase the grain profitability. The determination of genetic associations among domestic cultivars is facilitated by molecular markers. Data on genetic polymorphism is valuable for the germplasm association and regarding the developing management strategies. The information would be supportive for potential genome mapping programs and for the relevance of intellectual property rights of wheat breeders. Present review is an effort for providing support information to wheat breeders to develop varieties with varied genetic environment to attain continuity in large-scale wheat production. In this review, we have tried to provide a collective depiction of relevant information about the usage of some commonly used markers in wheat. It may help researchers to find out the frequentness and application of different markers and compare their results. The manuscript may serve as a platform helping the intellectuals for the selection and modification of their marker system in wheat diversity analysis. The heart of this review is the emphasis on the performance of various molecular genetic markers in diversity studies in relation to definite approaches that are in practice since several years allied with the multifaceted wheat molecular breeding and its polyploid nature.


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Dizkirici, Ayten; Kansu, Cigdem; Önde, Sertaç; AVCI BİRSİN, MELAHAT; Ozgen, Murat; Kaya, Zeki (Springer Science and Business Media LLC, 2013-12-01)
Cultivated wheat, (Triticum aestivum L.), is one of the most important food crops in the world. The Aegilops L. genus is frequently utilized by plant breeders for improving the current wheat cultivars due to their close relationships. Therefore, understanding the phylogenetic relationships among the species of these genera is not only valuable for plant taxonomy, but also for plant breeding efforts. The presented phylogenetic analysis was based on the sequences of trnT-F chloroplast DNA containing three non...
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Kavas, Musa; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biology (2011)
Soil salinity negatively affects agricultural production in Turkey by decreasing the yield and quality. Direct introduction of stress related genes by genetic engineering is one of the most rapid approaches to develop stress tolerant crops. In this study, TaNHX1 gene was isolated from bread wheat and three different local wheat cultivars were transformed with overexpression vectors containing TaNHX1 gene by using Agrobacterium-mediated and particle bombardment gene transfer techniques. Immature embryo and i...
Impact of biased sex ratio on the genetic diversity, structure, and differentiation of Populus nigra (European black poplar)
Yelmen, Burak; Değirmenci, Funda Ö.; Kaya, Zeki (Canadian Science Publishing, 2020-10-01)
Effective population size is a crucial concept of conservation biology. It is reduced by biased sex ratio, consequently causing loss of genetic variation. To evaluate genetic diversity related to gender, and investigate the possible effects of biased sex ratio, we analyzed available microsatellite DNA markers from 120 samples of Populus nigra L. (European black poplar) originating from five geographical regions in Turkey. Using 12 microsatellite markers, we detected 60 clones of the same genotype, out of 12...
Diallel analysis of wheat parents and their F-2 progenies under medium and low level of available N in soil
Yildirim, Mehmet; Bahar, Bilge; Genc, Ibrahim; Korkmaz, Kursat; Karnez, Ebru (Informa UK Limited, 2007-01-01)
Due to high production cost and prevention of environmental pollution, it is important to reduce the amount of nitrogen (N) fertilizer used on wheat (Triticum aestivum L.). The aim of this study was to evaluate N use of 6 x 6 diallel wheat F-2 progenies and parental lines at low (NO, no N fertilizer) and high (N+, 160 kg N ha(-1)) N levels. Significant differences were found between N+ and NO application in grain N content, grain N yield, N use efficiency for grain N yield (NUEgn), and N use efficiency for ...
Genetic relationships among perennial and annual Cicer species growing in Turkey as revealed by allozymes
Sudupak, MA; Kence, Aykut (Springer Science and Business Media LLC, 2004-05-01)
Allozyme polymorphisms were used to assess genetic variation and relationships among ten Cicer species ( annuals and perennials) growing in Turkey. Using seven enzyme systems, 12 putative scorable loci were detected and surveyed for polymorphism in an accession collection including wild and cultivated forms. Variation was generally low within accessions and species, but common between species. Cluster analysis based on the pairwise genetic distance coefficients (Nei 1978) among accessions and species using ...
Citation Formats
M. K. Khan, A. Pandey, S. Choudhary, E. E. HAKKI, M. Akkaya, and G. Thomas, “From RFLP to DArT: molecular tools for wheat (Triticum spp.) diversity analysis,” GENETIC RESOURCES AND CROP EVOLUTION, pp. 1001–1032, 2014, Accessed: 00, 2020. [Online]. Available: