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Cloning and heterologous expression of the extracellular alpha-galactosidase from Aspergillus fumigatus in Aspergillus sojae under the control of gpdA promoter
Date
2010-07-01
Author
Gurkok, Suemeyra
Soyler, Betuel
Biely, Peter
Ögel, Zümrüt Begüm
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Aspergillus fumigatus is highly pathogenic especially for immunocompromised people however it can efficiently produce many industrially important enzymes. The gene coding et-galactosidase enzyme (aglB) of A. fumigatus IMI 385708 has been cloned onto pAN52-4 fungal expression vector and expressed in a GRAS organism, Aspergillus sojae ATCC11906 under the control of constitutive glyceraldehyde-3-phosphate dehydrogenase (gpdA) promoter. pAN52-4 fungal expression system allowed high level alpha-galactosidase production in media with simple sugar glucose as the sole carbon source and without a requirement for an inducer with a yield of 2.45 U/ml which is nearly 3-fold higher than the yield obtained from A. fumigatus grown in locust bean gum containing medium.
Subject Keywords
Process Chemistry and Technology
,
Biochemistry
,
Bioengineering
,
Catalysis
URI
https://hdl.handle.net/11511/56499
Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
DOI
https://doi.org/10.1016/j.molcatb.2009.09.012
Collections
Graduate School of Natural and Applied Sciences, Article