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Cloning and heterologous expression of the extracellular alpha-galactosidase from Aspergillus fumigatus in Aspergillus sojae under the control of gpdA promoter

2010-07-01
Gurkok, Suemeyra
Soyler, Betuel
Biely, Peter
Ögel, Zümrüt Begüm
Aspergillus fumigatus is highly pathogenic especially for immunocompromised people however it can efficiently produce many industrially important enzymes. The gene coding et-galactosidase enzyme (aglB) of A. fumigatus IMI 385708 has been cloned onto pAN52-4 fungal expression vector and expressed in a GRAS organism, Aspergillus sojae ATCC11906 under the control of constitutive glyceraldehyde-3-phosphate dehydrogenase (gpdA) promoter. pAN52-4 fungal expression system allowed high level alpha-galactosidase production in media with simple sugar glucose as the sole carbon source and without a requirement for an inducer with a yield of 2.45 U/ml which is nearly 3-fold higher than the yield obtained from A. fumigatus grown in locust bean gum containing medium.