Optimal conditions for enhanced beta-mannanase production by recombinant Aspergillus sojae

Date

2010-07-01

Author

Ozturk, Bengu
Çekmecelioğlu, Deniz
Ogel, Zumrut Begum

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

139
views

0
downloads

Optimization of the growth conditions for maximum P-mannanase production in shake flasks by using recombinant Aspergillus sojae ATCC11906 (AsT1) was carried out by Box-Behnken design of response surface methodology. The highest beta-mannanase activity on the fourth day of cultivation at 30 degrees C was obtained as 363 U/ml in the optimized medium consisting of 7% sugar beet molasses, 0.43% NH(4)NO(3), 0.1% K(2)HPO(4) and 0.05% MgSO(4) (by weight per volume) at 207 rpm. On the sixth day of cultivation under the optimized conditions, the highest beta-mannanase activity was achieved as 482 U/ml which is 1.4-fold of 352 U/ml activity found on glucose medium previously. (C) 2010 Elsevier BM. All rights reserved.

Subject Keywords

Process Chemistry and Technology, Biochemistry, Bioengineering, Catalysis

URI

https://hdl.handle.net/11511/34616

Journal

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

DOI

https://doi.org/10.1016/j.molcatb.2010.02.009

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Immobilization of dioxomolybdenum(VI) complex bearing salicylidene 2-picoloyl hydrazone on chloropropyl functionalized SBA-15: A highly active, selective and reusable catalyst in olefin epoxidation Bagherzadeh, Mojtaba; Zare, Maryam; Salemnoush, Taghi; Özkar, Saim; Akbayrak, Serdar (Elsevier BV, 2014-04-05) A novel organic-inorganic hybrid heterogeneous catalyst system was obtained from the reaction of the molybdenum(VI) complex of salicylidene 2-picoloyl hydrazone with mesoporous silica containing 3-chloropropyl groups prepared by a direct synthetic approach involving hydrolysis and co-condensation of tetraethylorthosilicate (TEOS) and 3-chloropropyltrimethoxysilane in the presence of the triblock copolymer P123 as template under acidic conditions. Characterization of the functionalized materials by X-ray dif...
Exceptionally thermally stable, hydrocarbon soluble Ziegler-type Ir(0)(n) nanoparticle catalysts made from [Ir(1,5-COD)(mu-O2C8H15)](2) plus AlEt3: Tests of key hypotheses for their unusual stabilization Hamdemir, Isil K.; Özkar, Saim; Finke, Richard G. (Elsevier BV, 2013-11-01) In recent work we showed that Ziegler-type nanoparticles made from [Ir(1,5-COD)(mu-O2C8H15)](2) plus AlEt3 are an unusually thermally stable (>= 30 min at 200 degrees C), hydrocarbon-solvent soluble, high catalytic activity nanoparticle catalyst (I.K. Hamdemir, S. Ozkar, K.-H. Yih, J.E. Mondloch, R.G. Finke, ACS Catal. 2 (2012) 632-641). As such, they are analogous to-and currently the cleanest and best characterized model system for-Ziegler-type nanoparticles made from Co or Ni precatalysts plus AlEt3 whic...
Enantioselective synthesis of 4,5,6,7-tetrahydro-4-oxo-benzofuran-5-yl acetate and 1-benzyl-4,5,6,7-tetrahydro-4-oxo-1(H)-indol-5-yl acetate using chemoenzymatic methods Demir, Ayhan Sıtkı; Caliskan, Zerrin; Sahin, Ertan (Elsevier BV, 2007-03-01) The chemoenzymatic synthesis of both of the enantiomers of pharmacologically interesting compounds such as 4,5,6,7-tetrahydro-4-oxobenzofuran-5-yl acetate (2a), 4,5,6,7-tetrahydro-4-oxo-6,6-dimethylbenzofuran-5-yl acetate (2b), and their hydroxy derivatives 3a, 3b, 1-benzyl4,5,6,7-tetrahydro-4-oxo-1(H)-indol-5-yl acetate (5), starting from 6,7-dihydrobenzofuran-4(5H)-one (la), 6,7-dihydro-6,6-dimethylbenzofuran4(5H)-one (7b), and 1-benzyl-6,7-dihydro-1 H-indol-4(5H)-one (4) are reported. Manganese(III) acet...
Cloning and heterologous expression of the extracellular alpha-galactosidase from Aspergillus fumigatus in Aspergillus sojae under the control of gpdA promoter Gurkok, Suemeyra; Soyler, Betuel; Biely, Peter; Ögel, Zümrüt Begüm (Elsevier BV, 2010-07-01) Aspergillus fumigatus is highly pathogenic especially for immunocompromised people however it can efficiently produce many industrially important enzymes. The gene coding et-galactosidase enzyme (aglB) of A. fumigatus IMI 385708 has been cloned onto pAN52-4 fungal expression vector and expressed in a GRAS organism, Aspergillus sojae ATCC11906 under the control of constitutive glyceraldehyde-3-phosphate dehydrogenase (gpdA) promoter. pAN52-4 fungal expression system allowed high level alpha-galactosidase pro...
Fusarium roseum and Aspergillus oryzae-mediated enantioselective reduction of benzils to benzoins Elmir, Ayhan S.; Ayhan, Peruze; Demirtas, Umut; Erkilic, Umut (Elsevier BV, 2008-11-01) Aspergillus oryzae OUT5048 and Fusarium roseum OUT4019 were found to be effective biocatalysts in the reduction of benzils to optically active benzoins. Easily available symmetrical benzil derivatives were reduced to the corresponding benzoins [(S)-2-hydroxy-1,2-diphenylethanones] by A. oryzae OUT5048 with tip to 94% ee and by F roseum OUT4019 with up to 98% ee, respectively. In this Work. first general method for whole-cell-mediated selective reduction of benzils to benzoins is reported. It is also shown t...

Citation Formats

B. Ozturk, D. Çekmecelioğlu, and Z. B. Ogel, “Optimal conditions for enhanced beta-mannanase production by recombinant Aspergillus sojae,” JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, pp. 135–139, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/34616.