Optimal conditions for enhanced beta-mannanase production by recombinant Aspergillus sojae

Date

2010-07-01

Author

Ozturk, Bengu
Çekmecelioğlu, Deniz
Ogel, Zumrut Begum

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Optimization of the growth conditions for maximum P-mannanase production in shake flasks by using recombinant Aspergillus sojae ATCC11906 (AsT1) was carried out by Box-Behnken design of response surface methodology. The highest beta-mannanase activity on the fourth day of cultivation at 30 degrees C was obtained as 363 U/ml in the optimized medium consisting of 7% sugar beet molasses, 0.43% NH(4)NO(3), 0.1% K(2)HPO(4) and 0.05% MgSO(4) (by weight per volume) at 207 rpm. On the sixth day of cultivation under the optimized conditions, the highest beta-mannanase activity was achieved as 482 U/ml which is 1.4-fold of 352 U/ml activity found on glucose medium previously. (C) 2010 Elsevier BM. All rights reserved.

Subject Keywords

Process Chemistry and Technology, Biochemistry, Bioengineering, Catalysis

URI

https://hdl.handle.net/11511/34616

Journal

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

DOI

https://doi.org/10.1016/j.molcatb.2010.02.009

Collections

Department of Food Engineering, Article

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Citation Formats

B. Ozturk, D. Çekmecelioğlu, and Z. B. Ogel, “Optimal conditions for enhanced beta-mannanase production by recombinant Aspergillus sojae,” JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, pp. 135–139, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/34616.