Differentiation of mesophilic and thermophilic bacteria with Fourier transform infrared spectroscopy

Date

2007-02-01

Author

Garip, Sebnem
Bozoglu, Faruk
Severcan, Feride

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

119
views

0
downloads

In the present study the characterization and differentiation of mesophilic and thermophilic bacteria were investigated by using Fourier transform infrared (FT-IR) spectroscopy. Our results showed significant differences between the FT-IR spectra of mesophilic and thermophilic bacteria. The protein-to-lipid ratio was significantly higher for thermophiles compared to mesophiles. The absorption intensity of the CH3 asymmetric stretching vibration was higher in thermophilic bacteria, indicating a change in the composition of the acyl chains. The higher intensity/area observed in the CH2 symmetric stretching mode at 2857 cm(-1), and the CH2 bending vibration band at 1452 cm(-1), indicated a higher amount of saturated lipids in thermophilic bacteria. The lipid C=O stretching vibration at 1739 cm(-1), which was observed in the mesophilic group, was not observed clearly in the thermophilic group, indicating a difference in packing that is presumably due to the decreased proportion of unsaturated acyl chains in thermophilic bacteria. In addition, the carbonyl groups become hydrogen bonded and the cellular DNA content was lower in thermophilic bacteria. Moreover, in the 1000-400 cm(-1) frequency region, the spectra of each bacterial species belonging to both the mesophilic and thermophilic bacterial groups, showed characteristic differences that were discriminated via dendrogram using cluster analysis. The curent study implies that FT-IR spectroscopy could be succesfuly applied for the rapid comparison of bacterial groups and species to establish either similarities or discrepencies, as well as to confirm biochemical or physiological characteristics.

Subject Keywords

Instrumentation, Spectroscopy

URI

https://hdl.handle.net/11511/56834

Journal

APPLIED SPECTROSCOPY

DOI

https://doi.org/10.1366/000370207779947486

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Surface-enhanced Raman of dopamine and neurotransmitters using sol-gel substrates and polymer-coated fiber-optic probes Volkan, Mürvet; Vo-Dinh, T (SAGE Publications, 2000-12-01) Surface-enhanced Raman spectroscopy (SERS) is used to detect catecholamines-viz., dopamine, norepinephrine, epinine, and isoproterenol-using a new substrate prepared with sol-gel technology. Catecholamines are complexed at the surface by iron(III) ions, which were introduced during the reduction of the silver ions embedded inside the sol-gel matrix. With the use of a He-Ne laser at a power of only 12 mW, it is possible to measure dopamine in the micromolar concentration range. A single-fiber SERS probe syst...
Interactions of tamoxifen with distearoyl phosphatidylcholine multilamellar vesicles: FTIR and DSC studies Bilge, Duygu; ŞAHİN, İPEK; KAZANCI, NADİDE; Severcan, Feride (Elsevier BV, 2014-09-15) Interactions of a non-steroidal antiestrogen drug, tamoxifen (TAM), with distearoyl-sn-glycero-3-phosphatidylcholine (DSPC) multilamellar liposomes (MLVs) were investigated as a function of drug concentration (1-15 mol%) by using two noninvasive techniques, namely Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). FTIR spectroscopy results show that increasing TAM concentrations (except 1 mol%) increased the wavenumbers of the CH2 stretching modes, implying an disord...
Analysis of the Raman intensities near the phase transitions in ammonium halides Yurtseven, Hasan Hamit (Elsevier BV, 2008-12-01) This study concentrates on the temperature dependence of the Raman intensities for the lattice modes in ammonium halides (NH4Cl and NH4Br) close to phase transitions, We predict their intensities using the results of a shell model for the Raman polarizability within the framework of an Ising pseudospin-phonon coupled model.
Disorder-induced Raman scattering in ammonium halides Yurtseven, Hasan Hamit (Informa UK Limited, 1995-01-01) The mechanism responsible for the lambda-phase transitions in ammonium halides is investigated within the framework of an extended theory of the disorder-induced Raman scattering. The Raman spectra of ammonium halides have been predicted for both the disordered beta phase (NH4Cl and NH4Br) and the ordered gamma phase (NH4Br). It is postulated that those terms which dominate the dynamic distortions of the lattices and thereby cause the bulk of the disorder-allowed Raman scattering, will also dominate the qua...
The lambda-phase transition in NH4Br Yurtseven, Hasan Hamit; Sherman, WF (Informa UK Limited, 1996-01-01) This study gives the predicted frequencies for the Raman modes of v(7)TA(56 cm(-1)), v(5)LO(177 cm(-1)) and v(2)(1684 cm(-1)) of NH4Br at various temperatures for zero pressure across the lambda-phase transition in this crystal system. We calculate the Raman frequencies by means of a constant mode Gruneisen parameter for each mode across the lambda-phase change region of NH4Br.

Citation Formats

S. Garip, F. Bozoglu, and F. Severcan, “Differentiation of mesophilic and thermophilic bacteria with Fourier transform infrared spectroscopy,” APPLIED SPECTROSCOPY, pp. 186–192, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/56834.