Melatonin strongly interacts with zwitterionic model membranes - evidence from Fourier transform infrared spectroscopy and differential scanning calorimetry

Date

2005-03-01

Author

Severcan, Feride
Sahin, I
Kazanci, N

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

150
views

0
downloads

Interactions of melatonin with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) multilamellar liposomes (MLVs) were investigated as a function of temperature and melatonin concentration (1-30 mol%) by using two noninvasive techniques, namely Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). The investigation of the C-H, C=O, and PO2- antisymmetric double stretching modes in FTIR spectra and DSC studies reveal that melatonin changes the physical properties of the DPPC bilayers by decreasing the main phase transition temperature, abolishing the pretransition, ordering the system in the gel phase, and increasing the dynamics of the system both in the gel and liquid crystalline phases. It also causes significant decrease in the wavenumber for the C=O stretching and PO2- antisymmetric double bond stretching bands, which indicates strong hydrogen bonding The results imply that melatonin locates in the interfacial region of the membrane. Furthermore, in the DSC curve, more than one signal is observed at high melatonin concentrations (24 and 30 mol%), which indicates melatonin-induced phase separation in DPPC membranes.

Subject Keywords

Melatonin, Dipalmitoyl phosphatidylcholine, Membrane, Liposome, Fourier transform infrared, Differential scanning calorimetry, Phase separation

URI

https://hdl.handle.net/11511/57412

Journal

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

DOI

https://doi.org/10.1016/j.bbamem.2004.12.009

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Melatonin induces opposite effects on order and dynamics of anionic DPPG model membranes Sahin, Ipek; Severcan, Feride; Kazanci, Nadide (Elsevier BV, 2007-05-27) The temperature and concentration induced effects of melatonin on anionic dipalmitoyl phosphatidylglycerol (DPPG) multilamellar liposomes (MLVs) were investigated by using Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). The results show that melatonin does not perturb the phase transition profile, while a decrease in the main transition temperature (T-m) is noticed at high melatonin concentrations (15, 24 and 30 mol Low concentrations of melatonin (3, 6 and 9 mol ...
Competitive effect of vitamin D-2 and Ca2+ on phospholipid model membranes: an FTIR study Toyran, N; Severcan, Feride (2003-04-01) The interaction of Ca2+, with dipalmitoyl phosphatidylcholine (DPPC) model membranes was studied in the presence and absence of vitamin D-2 by using Fourier transform infrared spectroscopy. Addition of vitamin D-2 and/or Ca2+ into pure DPPC liposomes shifts the phase transition to higher temperature, orders and decreases the dynamics of the acyl chains in both phases and does not induce hydrogen bond formation in the interfacial region. Moreover, the dynamics of the head group of the phospholipid decreases ...
Biophysical studies of progesterone-model membrane interactions Korkmaz, Filiz; Kırbıyık, Halil; Severcan, Feride; Department of Physics (2003) Interactions of progesterone with zwitterionic dipalmitoyl phosphotidylcholine (DPPC) multilamellar liposomes (MLVs) were investigated as a function of temperature and progesterone concentration by using three non-invasive techniques of Fourier transform infrared (FTIR) spectroscopy, turbidity at 440 nm and differential scanning calorimetry (DSC).The results show that lmol% of progesterone does not induce a significant change in the shape of thermotropic profile of DPPC. However as progesterone concentratio...
Thermal Characterization of Sepiolite Samples Kök, Mustafa Verşan (2013-01-15) In this research, two sepiolite samples of different origins were studied by X-ray diffraction, Fourier transform infrared spectroscopy, differential scanning calorimeter, and thermogravimetry. It was observed that both sepiolite samples are nearly pure and well crystallized sepiolite. Thermal studies (thermogravimetry) showed that four distinct weight loss regions are observed in both sepiolite samples studied. It was noticed that these structural changes play an important role to the properties and uses o...
Tamoxifen-model membrane interactions: An FT-IR study Boyar, H; Severcan, Feride (1997-06-01) The temperature- and concentration-induced effects of tamoxifen (TAM) on dipalmitoyl phosphatidylcholine (DPPC) model membranes were investigated by the Fourier transform-infrared (FT IR) spectroscopic technique. An investigation of the C-H stretching region and the C=0 mode reveals that the inclusion of TAM changes the physical properties of the DPPC multibilayers by (i) shifting the main phase transition to lower temperatures; (ii) broadening the transition profile slightly; (iii) disordering the system i...

Citation Formats

F. Severcan, I. Sahin, and N. Kazanci, “Melatonin strongly interacts with zwitterionic model membranes - evidence from Fourier transform infrared spectroscopy and differential scanning calorimetry,” BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, pp. 215–222, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57412.