Melatonin strongly interacts with zwitterionic model membranes - evidence from Fourier transform infrared spectroscopy and differential scanning calorimetry

Date

2005-03-01

Author

Severcan, Feride
Sahin, I
Kazanci, N

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Interactions of melatonin with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) multilamellar liposomes (MLVs) were investigated as a function of temperature and melatonin concentration (1-30 mol%) by using two noninvasive techniques, namely Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). The investigation of the C-H, C=O, and PO2- antisymmetric double stretching modes in FTIR spectra and DSC studies reveal that melatonin changes the physical properties of the DPPC bilayers by decreasing the main phase transition temperature, abolishing the pretransition, ordering the system in the gel phase, and increasing the dynamics of the system both in the gel and liquid crystalline phases. It also causes significant decrease in the wavenumber for the C=O stretching and PO2- antisymmetric double bond stretching bands, which indicates strong hydrogen bonding The results imply that melatonin locates in the interfacial region of the membrane. Furthermore, in the DSC curve, more than one signal is observed at high melatonin concentrations (24 and 30 mol%), which indicates melatonin-induced phase separation in DPPC membranes.

Subject Keywords

Melatonin, Dipalmitoyl phosphatidylcholine, Membrane, Liposome, Fourier transform infrared, Differential scanning calorimetry, Phase separation

URI

https://hdl.handle.net/11511/57412

Journal

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

DOI

https://doi.org/10.1016/j.bbamem.2004.12.009

Collections

Department of Biology, Article

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Citation Formats

F. Severcan, I. Sahin, and N. Kazanci, “Melatonin strongly interacts with zwitterionic model membranes - evidence from Fourier transform infrared spectroscopy and differential scanning calorimetry,” BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, pp. 215–222, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57412.