Virus induced gene silencing in Brachypodium distachyon, a model organism for cereals

Demircan, Turan
Akkaya, Mahinur
Brachypodium distachyon is emerging as a model organism for crops as a better alternative to Oryzae sativa. It shares common characteristics of a model plant with its small genome, small physical plant size, a short lifecycle, and less demanding growth requirements similar to Arabidopsis thaliana. In this study, we are reporting for the first time, an implementation of virus induced gene silencing (VIGS), a powerful method allowing rapid and effective means of loss of gene function through RNA interference. To this end, Phytoene desaturase (Pds) gene, commonly preferred in gene silencing studies as a phenotypic marker, was silenced using Barley Stripe Mosaic Virus (BSMV), the most effectively used virus in monocots for VIGS., a fragment of the ORF of the B. distachyon Pds gene was cloned into the BSMV vector having the gamma genome proviral DNA. The decreased Pds gene expression was confirmed after plant infection by qRT-PCR. The effectiveness of BSMV infection was also tested with the transcripts of the vector constructed for GFP expression. We believe the demonstration of BSMV mediated VIGS will be an important step for evaluating functions of crop genes in this model organism.


The effect of hybrid vigor on callus induction and plant regeneration from mature embryo culture of barley (Hordeum vulgare)
Ozgen, M; Birsin, MA; Onde, S (Springer Science and Business Media LLC, 2005-07-01)
The influence of hybrid vigor on callus induction, callus weight, regeneration capacity of callus, culture efficiency and number of regenerated plants from mature embryo cultures of winter barley ( Hordeum vulgare L.) hybrid was studied. A total of 15 F-1 hybrids and their parents were used for mature embryo culture. The statistical analysis of the results revealed that positive heterosis was noticed for all these characters except for callus weight. Plants regenerated in vitro were successfully established...
Consequences of habitat fragmentation on genetic diversity and structure of Salix alba L. populations in two major river systems of Turkey
Degirmenci, Funda O.; Acar, Pelin; Kaya, Zeki (Springer Science and Business Media LLC, 2019-08-01)
Salix alba L. (white willow) is an indicator species of a healthy riparian ecosystem with great renewable energy potential in Turkey though habitats of the species in many river ecosystems are highly degraded or fragmented. Impacts of this degradation of river ecosystems on the magnitude and pattern of genetic diversity are not known. This study was aimed at assessing the genetic structure of S. alba populations in two highly degraded and fragmented river systems (the Goksu and Kzlrmak rivers) in Turkey wit...
Molecular and Elemental Characterization of Selected Turkish Durum Wheat Varieties
HAKKI, ERDOĞAN EŞREF; Dograr, Nurdan; Pandey, Anamika; Khan, Mohd. Kamran; HAMURCU, MEHMET; KAYIŞ, SEYİT ALİ; GEZGİN, SAİT; Olmez, Fatih; Akkaya, Mahinur (AcademicPres (EAP) Publishing House, 2014-07-01)
Combination of elemental and protein studies along with molecular data using microsatellite markers may lead CO the better and realistic determination of relatedness between the varieties and their populations. In this study, the extent of diversity among five Turkish durum wheat cultivars and their populations has been assessed using seven microsatellite markers and the elemental analysis together with the differences in their protein content. In molecular analysis, total 23 alleles have been obtained amon...
Signal transduction pathways mediated by the interaction of CpG DNA with Toll-like receptor 9.
Takeshita, F; Gursel, I; Ishii, KJ; Suzuki, K; Gürsel, Mayda; Klinman, DM (2004-02-01)
Synthetic oligodeoxynucleotides (ODN) expressing non-methylated "CpG motifs" patterned after those present in bacterial DNA have characteristic immunomodulatory effects. CpG DNA is recognized as a pathogen-associated molecular pattern, and triggers a rapid innate immune response. CpG ODN are being harnessed for a variety of therapeutic uses, including as immune adjuvants, for cancer therapy, as anti-allergens, and as immunoprotective agents. The signal transduction pathway mediated by the engagement of CpG ...
Reciprocal effects in anther cultures of wheat hybrids
YILDIRIM, MEHMET; Bahar, B.; Genc, I.; HATİPOĞLU, RÜŞTÜ; Altintas, S. (Institute of Experimental Botany, 2008-12-01)
This study was conducted to determine the reciprocal effects for anther culture response in wheat (Triticum aestivum L.) using a set of 4 x 4 full diallel crosses. Both reciprocal and nuclear genetic effects were highly significant for anther culture response and useful for selection and breeding purposes. General combining ability (GCA) effects were predominant for all investigated anther culture traits. Also, significant differences for specific combining ability (SCA) effects were detected between recipr...
Citation Formats
T. Demircan and M. Akkaya, “Virus induced gene silencing in Brachypodium distachyon, a model organism for cereals,” PLANT CELL TISSUE AND ORGAN CULTURE, pp. 91–96, 2010, Accessed: 00, 2020. [Online]. Available: