Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins

Date

2009-01-22

Author

Altindis, Emrah
Tefon, Burcu E.
Yildirim, Volkan
Ozcengiz, Erkan
Becher, Doete
Hecker, Michael
Özcengiz, Gülay

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Bordetella pertussis is the causative agent of highly communicable respiratory infection whooping cough (pertussis) which remains one of the world's leading causes of vaccine-preventable deaths. In the present study, total soluble proteins extracted from two B. pertussis strains, Tohama I and the local isolate Saadet were separated by two-dimensional gel electrophoresis and analyzed by Western blotting for their reactivity with the antisera obtained from the mice immunized with inactivated whole cells as well as those collected from the mice challenged intraperitoneally with live cells of each strain. Of a total of 25 immunogenic proteins identified, 21 were shown to be the novel antigens for B. pertussis.

Subject Keywords

Public Health, Environmental and Occupational Health, General Immunology and Microbiology, Molecular Medicine, General Veterinary, Infectious Diseases

URI

https://hdl.handle.net/11511/57688

Journal

VACCINE

DOI

https://doi.org/10.1016/j.vaccine.2008.11.020

Collections

Department of Biology, Article

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Citation Formats

E. Altindis et al., “Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins,” VACCINE, pp. 542–548, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57688.