Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals

Date

2007-01-01

Author

Saatci, Ebru
Nistor, Mihaela
Gaspar, Szilveszter
Csoregi, Elisabeth
İşcan, Mesude

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This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)(6)) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1 fM to 1mM for Zn2+, and 10pM to 1mM for Cd2+. The GST-(His) 6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1 fM to 10 mu M, and Hg2+ in the range of 1 fM to 10mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His) 6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors for the detection of heavy metals.

Subject Keywords

Analytical Chemistry, Waste Management and Disposal, Public Health, Environmental and Occupational Health, Soil Science, Pollution, Health, Toxicology and Mutagenesis, Water Science and Technology, Environmental Chemistry

URI

https://hdl.handle.net/11511/58046

Collections

Department of Biology, Article