Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals

Date

2007-01-01

Author

Saatci, Ebru
Nistor, Mihaela
Gaspar, Szilveszter
Csoregi, Elisabeth
İşcan, Mesude

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

160
views

0
downloads

This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)(6)) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1 fM to 1mM for Zn2+, and 10pM to 1mM for Cd2+. The GST-(His) 6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1 fM to 10 mu M, and Hg2+ in the range of 1 fM to 10mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His) 6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors for the detection of heavy metals.

Subject Keywords

Analytical Chemistry, Waste Management and Disposal, Public Health, Environmental and Occupational Health, Soil Science, Pollution, Health, Toxicology and Mutagenesis, Water Science and Technology, Environmental Chemistry

URI

https://hdl.handle.net/11511/58046

Journal

INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY

DOI

https://doi.org/10.1080/03067310701409309

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Quantum chemical treatment of cyanogen azide and its univalent and divalent ionic forms Türker, Burhan Lemi; Atalar, Taner (Elsevier BV, 2008-05-30) An explosive material, cyanogen azide (CN4) and its univalent and divalent anionic and cationic forms have been studied quantum chemically by using different theoretical approaches. In this study, the structures considered have been screened for their relative stabilities. Also, they have been investigated whether the charged forms play a role in the usual explosion process or any electrical charging during storage cause explosion. Various quantum chemical properties are obtained and discussed. It has been ...
Investigation of sludge viscosity and its effects on the performance of a vacuum rotation membrane bioreactor KOMESLİ, Okan Tarık; Gökçay, Celal Ferdi (Informa UK Limited, 2014-03-04) Sludge characteristics of a full-scale vacuum rotation membrane (VRM) bioreactor having plate-type membranes with 0.038 m nominal pore size and 540 m(2) surface area were investigated. The VRM plant is composed of an aeration tank and a filtration chamber. The sludge floc size distribution, as determined microscopically, was mainly between 0 and 100 m in the filter chamber with very little difference in size distribution between summer (20-25 degrees C) and winter (10-15 degrees C) seasons. Small floc size ...
An ab initio study on ethylenedinitramine and its monovalent ions Türker, Burhan Lemi (Elsevier BV, 2005-02-14) An explosive material, ethylenedinitramine (EDNA), its mono and di aci forms, as well as its monovalent cation and anion forms have been considered for 6-31G (UHF) type ab initio quantum chemical treatment in order to investigate the stability of EDNA in the neutral form and when charges develop on it exposed to electrical fields during the storage, handling or explosion process. The calculations indicate stable species. The aci forms are less stable than EDNA itself and the anion form is more stable than t...
Evaluation of PCB dechlorination pathways in anaerobic sediment microcosms using an anaerobic dechlorination model Demirtepe, Hale; Kjellerup, Birthe; Sowers, Kevin R.; İmamoğlu, İpek (Elsevier BV, 2015-10-15) A detailed quantitative analysis of anaerobic dechlorination (AD) pathways of polychlorinated biphenyls (PCBs) in sediment microcosms was performed by applying an anaerobic dechlorination model (ADM). The purpose of ADM is to systematically analyze changes in a contaminant profile that result from microbial reductive dechlorination according to empirically determined dechlorination pathways. In contrast to prior studies that utilized modeling tools to predict dechlorination pathways, ADM also provides quant...
A theoretical study on vomitoxin and its tautomers Türker, Burhan Lemi; Guemues, Selcuk (Elsevier BV, 2009-04-15) In the present work, the structural and electronic properties of vomitoxin (deoxynivalenol, a mycotoxin) and all of its possible tautomers have been investigated by the application of B3LYP/6-31 G(d,p) type quantum chemical calculations. According to the results of the calculations, tautomer V-4 has been found to be the most stable one among all the structures both in the gas and aqueous phases. The calculations also indicated that, vomitoxin and V-2 possess the deepest and the highest lying HOMO levels, re...

Citation Formats

E. Saatci, M. Nistor, S. Gaspar, E. Csoregi, and M. İşcan, “Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals,” INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, pp. 745–754, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/58046.