Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals

Saatci, Ebru
Nistor, Mihaela
Gaspar, Szilveszter
Csoregi, Elisabeth
İşcan, Mesude
This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)(6)) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1 fM to 1mM for Zn2+, and 10pM to 1mM for Cd2+. The GST-(His) 6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1 fM to 10 mu M, and Hg2+ in the range of 1 fM to 10mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His) 6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors for the detection of heavy metals.


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Citation Formats
E. Saatci, M. Nistor, S. Gaspar, E. Csoregi, and M. İşcan, “Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals,” INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, pp. 745–754, 2007, Accessed: 00, 2020. [Online]. Available: