Chemoenzymatic Conversion of Linoleic Acid into Conjugated Linoleic Acid

Date

2010-02-10

Author

Demir, Ayhan Sıtkı
Talpur, Farah N.

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An efficient chemoenzymatic method for preparing conjugated linoleic acid (CLA) using free linoleic acid (LA) as a substrate is described. In the first step, LA was transformed into 10-hydroxy-cis-12-octadecenoic acid (HA) by the whole cells of Lactobacillus plantarum after 48 h of incubation. The preincubation of whole cells with 0.03% LA resulted in a better yield of HA (480 mg/g) compared to cells grown without LA. In a second fast microwave step, HA was converted to cis-9, trans-11-octadecadienoic acid in the presence of iodine as a catalyst over a silica gel surface. The advantage of this method in preparing cis-9,trans-11 CLA is simple via the whole cell bioconversion of LA into HA via L. plantarum followed by the fast microwave-assisted synthesis of cis-9,trans-11 CLA in higher yields.

Subject Keywords

General Chemistry, General Agricultural and Biological Sciences

URI

https://hdl.handle.net/11511/62647

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

DOI

https://doi.org/10.1021/jf903521b

Collections

Department of Chemistry, Article

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Citation Formats

A. S. Demir and F. N. Talpur, “Chemoenzymatic Conversion of Linoleic Acid into Conjugated Linoleic Acid,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, pp. 1646–1652, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/62647.