Chemoenzymatic Conversion of Linoleic Acid into Conjugated Linoleic Acid

Demir, Ayhan Sıtkı
Talpur, Farah N.
An efficient chemoenzymatic method for preparing conjugated linoleic acid (CLA) using free linoleic acid (LA) as a substrate is described. In the first step, LA was transformed into 10-hydroxy-cis-12-octadecenoic acid (HA) by the whole cells of Lactobacillus plantarum after 48 h of incubation. The preincubation of whole cells with 0.03% LA resulted in a better yield of HA (480 mg/g) compared to cells grown without LA. In a second fast microwave step, HA was converted to cis-9, trans-11-octadecadienoic acid in the presence of iodine as a catalyst over a silica gel surface. The advantage of this method in preparing cis-9,trans-11 CLA is simple via the whole cell bioconversion of LA into HA via L. plantarum followed by the fast microwave-assisted synthesis of cis-9,trans-11 CLA in higher yields.


ABDULLAH, MI; YOUNG, JC; GAMES, DE (American Chemical Society (ACS), 1994-03-01)
Material obtained by the CO2 supercritical fluid extraction (SFE) of the wild and cultivated mushrooms Agaricus silvicola and Agaricus bisporus, respectively, was analyzed by g chromatography (GC)-mass spectrometry and GC-Fourier transform infrared spectrometry. SFE gave results comparable with classical extraction techniques for free fatty acids and esters but with fewer manipulative steps and also resulted in the characterization of acids not previously reported from these sources. The organic acids ident...
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Karacabey, Erkan; Mazza, Giuseppe (American Chemical Society (ACS), 2008-08-13)
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WANG, CF; TSAY, SM; LEE, CY; LIU, SM; ARAS, NK (American Chemical Society (ACS), 1992-06-01)
Phytate and trace element concentrations of the total daily diet of upper social groups in Taiwan were determined by P-31 FT NMR spectroscopy and by instrumental neutron activation analysis, respectively. Results of 15 samples are 2.21 +/- 0.71 g of phytate/kg of dry diet with the range 1.07-3.76 g/kg. The molar ratios of phytate to Zn and Ca were calculated, and bioavailabilities of these elements are discussed.
Chemoenzymatic synthesis of both enantiomers of 3-hydroxy-2,3-dihydro-4H-chromen-4-one
Demir, AS; Aybey, A; Sesenoglu, O; Polat, Faruk (Elsevier BV, 2003-06-06)
4-Oxo-3,4-dihydro-2-chromen-3-yl acetate is synthesized using manganese(III)acetate starting from 2,3-dihydro-4H-chromen-4-one. K2CO3 mediated hydrolysis of 4-oxo-3,4-dihdro-2-chromen-3-yl acetate furnished 3-hydroxy-2,3-dihydro-4H-chromen-4-one in high yield. The enantioselective hydrolysis of (+/-)-4-oxo-3,4-dihydro-2-chromen-3-yI acetate in various organic solvent-phosphate buffer (pH7) systems and enantioselective transesterification of (+/-)-3-hydroxy-2,3-dihydro-4H-chromen-4-one in organic solvents wa...
Experimental investigation of drag reduction effects of polymer additives on turbulent pipe flow using ultrasound Doppler velocimetry
Zeybek Vural, Serife; Bayram, Göknur; Uludağ, Yusuf (The Scientific and Technological Research Council of Turkey, 2014-01-01)
Drag reduction in fully developed turbulent pipe flow with 4 concentrations (200 to 500 wppm or mg/kg) of low molecular weight sodium carboxymethylcellulose (CMC) in aqueous solutions was investigated experimentally. Drag reduction was determined by pressure drop measurements. Maximum drag reduction achieved was 22% using 500 wppm CMC solution. To observe the impact of the presence of CMC on the flow, ultrasound Doppler velocimetry (UDV) was employed to monitor the instantaneous velocity distributions. Expe...
Citation Formats
A. S. Demir and F. N. Talpur, “Chemoenzymatic Conversion of Linoleic Acid into Conjugated Linoleic Acid,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, pp. 1646–1652, 2010, Accessed: 00, 2020. [Online]. Available: