Mechanism of Inhibition of CYP1A1 and Glutathione S-Transferase Activities in Fish Liver by Quercetin, Resveratrol, Naringenin, Hesperidin, and Rutin

Date

2015-01-02

Author

Arinc, Emel
Yilmaz, Duygu
BOZCAARMUTLU, AZRA

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Quercetin, resveratrol, naringenin, hesperidin, and rutin are phenolic compounds/flavonoids that may have roles in the reduction of cancer susceptibility. In this study, in vitro modulatory effects of them were studied on liver CYP1A1 associated 7-ethoxyresorufin-O-deethylase (EROD) activity and glutathione S-transferase (GST) activity of leaping mullet (Liza saliens). All of the phenolic compounds/flavonoids used exerted an inhibitory effect on both EROD and GST activities of fish. Quercetin, resveratrol, hesperidin, and rutin were found to inhibit EROD activity in a competitive manner; on the other hand, naringenin was found to inhibit EROD activity in a noncompetitive manner. Ki values of quercetin, resveratrol, naringenin, hesperidin, and rutin were calculated from Dixon plots as 0.12 mu M, 0.67 mu M, 2.63 mu M, 18 mu M and 0.1mM, respectively. Resveratrol, quercetin, and hesperidin were found to inhibit GST activity in a competitive manner; on the other hand, rutin and naringenin were found to inhibit GST activity in a mixed-type manner. Ki values of resveratrol, quercetin, hesperidin, naringenin, and rutin were calculated from Dixon plots as 3.2 mu M, 12.5 mu M, 45 mu M, 128 mu M, and 150 mu M, respectively. The results suggest that quercetin and resveratrol containing foods are effective in the prevention and treatment of cancer.

Subject Keywords

Enzymes, Dna-damage, Erod activity, Cytochromes p450, Metabolic-activation, O-deethylase, Dietary flavonoids, Leaping mullet, Mullet mugil-cephalus, Polycyclic aromatic-hydrocarbons

URI

https://hdl.handle.net/11511/66361

Collections

Department of Biology, Article