Microbial oil produced from biodiesel by-products could enhance overall production

Kiran, Esra Uckun
Trzcinski, Antoine
Webb, Colin
Glycerol and rapeseed meal, two major by-products of biodiesel production, have been tested for possible use as low-cost raw materials for the production of microbial bio-oil using the oleaginous yeast Rhodosporidium toruloides. Using fed-batch fermentation with crude glycerol and a novel nitrogen rich nutrient source derived from rapeseed meal as feed, it was shown that 13 g/L lipids could be produced, compared with 9.4 g/L when crude glycerol was used with yeast extract. When 100 g/L pure glycerol was used, the final lipid concentration was 19.7 g/L with the novel biomedium compared to 16.2 g/L for yeast extract. The novel biomedium also resulted in higher lipid yields (0.19 g lipid/g glycerol consumed compared to 0.12 g/L) suggesting it provides a better carbon to nitrogen balance for accumulating lipids. FAMEs produced from the microbial lipids indicated a high degree of unsaturation confirming that the fatty acids produced from the novel biomedium have potential for biodiesel production.


Enhancing the value of nitrogen from rapeseed meal for microbial oil production
Kiran, Esra Uckun; Salakkam, Apilak; Trzcinski, Antoine P.; Bakir, Ufuk; Webb, Colin (2012-05-10)
Rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. Various strategies were tested for the production of a novel fermentation medium, rich in free amino nitrogen (FAN): commercial enzymes (CEs) (2.7 mg g(-1) dry meal), liquid state fungal pre-treatment (LSF) using Aspergillus oryzae (4.6 mg g(-1)), liquid state fungal pre-treatment followed by fungal autolysis (LSF...
Microbial biodiesel production by direct methanolysis of oleaginous biomass
Thliveros, Panagiotis; Kiran, Esra Uckun; Webb, Colin (Elsevier BV, 2014-04-01)
Biodiesel is usually produced by the transesterification of vegetable oils and animal fats with methanol, catalyzed by strong acids or bases. This study introduces a novel biodiesel production method that features direct base-catalyzed methanolysis of the cellular biomass of oleaginous yeast Rhodosporidium toruloides Y4. NaOH was used as catalyst for transesterification reactions and the variables affecting the esterification level including catalyst concentration, reaction temperature, reaction time, solve...
Improvement of Pectinase Production by Co-culture of Bacillus spp. Using Apple Pomace as a Carbon Source
Kuvvet, Ceren; UZUNER, SİBEL; Çekmecelioğlu, Deniz (2019-05-01)
Increasing interest in using food and agricultural wastes for low cost enzyme production continues in fermentation industry. Bacteria, especially Bacillus subtilis, are known to produce pectinases in synthetic media and media amended with agricultural wastes as a carbon source, whereas the potential of co-culturing Bacillus species to produce pectinases has not been fully studied yet. In this study, co-culture of B. subtilis and B. pumilus was optimized for solid load and pH to maximize pectinase production...
Bacterial and fungal strain selections for cellulase and xylanase production using distillers' dried grains with solubles (DDGS)
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2020-01-01)
Distillers' dried grains with solubles (DDGS) are by-products of dry-mill bioethanol production from corn and wheat. The DDGS is leftover by the yeast after converting the starch into ethanol and it has a high nutritional profile including undigested fiber and protein fractions. The DDGS can be used as cheap microbial feedstock for the production of cellulase and xylanases, which are produced from expensive industrial feedstocks. In this study, screening of several bacterial and fungal strains was done usin...
Growth of microalgae Chlorella vulgaris at the photobioreactor for biodiesel production
Ertürk, Handan; Sudagıdan, Mert; Yurdakul, Merve; Öktem, Hüseyin Avni (2018-03-01)
Microalga is known to have higher lipid contents and biodiesel efficiency than most plant oil sources e.g. palm oil. We studied the growth of Chlorella vulgaris at the photobioreactor in our laboratory. We aimed to use this photobioreactor of laboratory scale as feed stream to an open pond larger scale bioreactor for future work. Photobioreactor had three compartments which had separate controls for light and air circulation. Temperature was kept at 22°C-26°C. The circulation rate was 180 L/h. The light int...
Citation Formats
E. U. Kiran, A. Trzcinski, and C. Webb, “Microbial oil produced from biodiesel by-products could enhance overall production,” BIORESOURCE TECHNOLOGY, pp. 650–654, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66388.