Bacterial and fungal strain selections for cellulase and xylanase production using distillers' dried grains with solubles (DDGS)

Date

2020-01-01

Author

Iram, Attia
Çekmecelioğlu, Deniz
Demirci, Ali

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Distillers' dried grains with solubles (DDGS) are by-products of dry-mill bioethanol production from corn and wheat. The DDGS is leftover by the yeast after converting the starch into ethanol and it has a high nutritional profile including undigested fiber and protein fractions. The DDGS can be used as cheap microbial feedstock for the production of cellulase and xylanases, which are produced from expensive industrial feedstocks. In this study, screening of several bacterial and fungal strains was done using DDGS as the feedstock for the hydrolytic enzyme production. Among all these strains, Aspergillus niger (NRRL 1956) produced the maximum cellulase of 0.592 IU/ml. On the other hand, stable high production of xylanase of 34.8 IU/ml was obtained by Aspergillus niger (NRRL 567) on day twelfth. Bacterial enzyme production by Bacillus subtilis (NRRL NSR352, DSM 17766, and NF1) was relatively lower (0-0.261 IU/ml for cellulase and 1.2-5.2 IU/ml for xylanase) than the fungal enzyme production. In conclusion, this study demonstrated that the hydrolyzed DDGS can be an alternative economical substrate for A. niger strains to produce cellulase and xylanase, respectively. The next phases of the study should further optimize the production of cellulase and xylanase in terms of growth parameters and medium components by using benchtop bioreactors.

URI

https://hdl.handle.net/11511/93568

DOI

https://doi.org/10.13031/aim.202000032

Conference Name

2020 ASABE Annual International Meeting

Collections

Department of Food Engineering, Conference / Seminar

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Distillers' dried grains with solubles (DDGS) and its potential as fermentation feedstock Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (Springer Science and Business Media LLC, 2020-07-01) Distillers' dried grain with solubles (DDGS) is a byproduct of bioethanol fermentation, which uses the dry milling technology for starch-rich grains such as corn, wheat, and barley. The current interest in bioethanol is increasing due to the need for renewable liquid fuels specifically in the transportation sector. Since DDGS is rich in crude protein, fat, fiber, vitamins, and minerals, it is currently used as aquaculture, livestock, and poultry feeds. In recent years, DDGS has been used as feedstock in the...
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Citation Formats

A. Iram, D. Çekmecelioğlu, and A. Demirci, “Bacterial and fungal strain selections for cellulase and xylanase production using distillers’ dried grains with solubles (DDGS),” presented at the 2020 ASABE Annual International Meeting, Virtual, Online, 2020, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/93568.