Enhancing the value of nitrogen from rapeseed meal for microbial oil production

Kiran, Esra Uckun
Salakkam, Apilak
Trzcinski, Antoine P.
Bakir, Ufuk
Webb, Colin
Rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. Various strategies were tested for the production of a novel fermentation medium, rich in free amino nitrogen (FAN): commercial enzymes (CEs) (2.7 mg g(-1) dry meal), liquid state fungal pre-treatment (LSF) using Aspergillus oryzae (4.6 mg g(-1)), liquid state fungal pre-treatment followed by fungal autolysis (LSFA) (9.13 mg g(-1)), liquid state pre-treatment using fungal enzymatic broth (EB) (2.1 mg g(-1)), but the best strategy was a solid state fungal pre-treatment followed by fungal autolysis (34.5 mg g(-1)).


Microbial oil produced from biodiesel by-products could enhance overall production
Kiran, Esra Uckun; Trzcinski, Antoine; Webb, Colin (2013-02-01)
Glycerol and rapeseed meal, two major by-products of biodiesel production, have been tested for possible use as low-cost raw materials for the production of microbial bio-oil using the oleaginous yeast Rhodosporidium toruloides. Using fed-batch fermentation with crude glycerol and a novel nitrogen rich nutrient source derived from rapeseed meal as feed, it was shown that 13 g/L lipids could be produced, compared with 9.4 g/L when crude glycerol was used with yeast extract. When 100 g/L pure glycerol was use...
Improvement of Pectinase Production by Co-culture of Bacillus spp. Using Apple Pomace as a Carbon Source
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Increasing interest in using food and agricultural wastes for low cost enzyme production continues in fermentation industry. Bacteria, especially Bacillus subtilis, are known to produce pectinases in synthetic media and media amended with agricultural wastes as a carbon source, whereas the potential of co-culturing Bacillus species to produce pectinases has not been fully studied yet. In this study, co-culture of B. subtilis and B. pumilus was optimized for solid load and pH to maximize pectinase production...
Evaluation and optimization of microbial oil production using synthetic sugars
Gürel, Nermin; Çekmecelioğlu, Deniz; Department of Food Engineering (2019)
Oleaginous microorganisms produce microbial oil that can be a solution to sustainable oil production. Lipomyces starkeyi DSM70295 and Rhodosporidium toruloides DSM4444 yeasts were chosen to produce microbial oil in this study. Synthetic sugars, glucose, galactose,xylose, fructose and 1:1(w/w) combinations of sugars with glucose were used in fermentation medium. Nitrogen is an important criterion for yeast growth and lipid accumulation. To test effects of nitrogen on yeast growth and lipid production, C/N ra...
Ca-D(-)-lactate production from orange baggase via enzymatic hydrolysis and fermentation
Baltacı, Saadet Fatma; Hamamcı, Haluk; Department of Food Engineering (2016)
The utilization of low-cost renewable carbohydrate sources for the production of lactic acid which is widely used organic acid in food and polymer industry is a recent strategy in bioeconomy. The objective of this work was to produce Ca-D(-) -lactate as a precursor for D(-)-polylactic acid through the bioconversion of orange bagasse (OB) by enzymatic hydrolysis and fermentation. The hydrolysis of OB carried with dried and fresh OB, and cellulolytic and pectinolytic enzymes at 55 C, 150 rpm and 4.8 pH to eva...
Optimization of bioethanol production from kitchen waste
Uncu, Oya Nihan; Çekmecelioğlu, Deniz; Department of Food Engineering (2009)
Kitchen waste, which is collected in large amounts from cafeterias, restaurants, dining halls, food processing plants, and household kitchens, have become a valuable material for bioprocess engineering. Due to the high carbohydrate fraction, kitchen waste has great potential to be used as a potential substrate for ethanol production. Utilization of it as a raw material in ethanol fermentation would also contribute to reduction of costs. In the first part of this study, the effect of pretreatment method and ...
Citation Formats
E. U. Kiran, A. Salakkam, A. P. Trzcinski, U. Bakir, and C. Webb, “Enhancing the value of nitrogen from rapeseed meal for microbial oil production,” ENZYME AND MICROBIAL TECHNOLOGY, pp. 337–342, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68164.