Resistance to anticancer drugs permanently alters electrophoretic mobility of cancer cell lines

Date

2017-04-01

Author

Kazan, Hasan Hueseyin
Urfali-Mamatoglu, Cagri
Guenduez, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

134
views

0
downloads

Electrophoretic mobility is a physical phenomenon defining the mobility of charged particles in a solution under applied electric field. As charged biological systems, living cells including both prokaryotes and eukaryotes have been assessed in terms of electrophoretic mobility to decipher their electrochemical structure. Moreover, determination of electrophoretic mobility of living cancer cells have promoted the advance exploration of the nature of the cancer cells and separation of cancer cells from normal ones under applied electric field. However, electrophoretic mobility of drug-resistant cells has not yet been examined. In the present study, we determined the electrophoretic mobility of drug-resistant cancer cell lines for both suspension and adherent cells and compared with those of drug-sensitive counterparts. We showed that resistance to anticancer drugs alters the electrophoretic mobility in a permanent manner, even lasting without any exposure to anticancer agents for a long time period. We also studied the cellular morphologies of adherent cells where the cellular invaginations and protrusions were increased in drug-resistant adherent cells, which could be direct cause of altered surface charge and electrophoretic mobility as a result. These findings could be helpful in terms of understanding the electrophysiological and physicochemical background of drug resistance in cancer cells and developing systems to separate drug-sensitive cells from drug-resistant ones.

Subject Keywords

Clinical Biochemistry, Biochemistry

URI

https://hdl.handle.net/11511/66466

Journal

ELECTROPHORESIS

DOI

https://doi.org/10.1002/elps.201600494

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Antioxidant enzyme activities in rat liver tissues of diabetic rats Sadi, Gökhan; Güray, Tülin; Department of Biochemistry (2004) Free radicals are the compounds having one or more unpaired electrons in their outer orbital and this unpaired electron make these compounds very reactive. Especially as their concentration increases, they initiate a chain oxidation reaction of lipids, proteins and nucleic acids. The condition, in which the production of free radicals exceeds their elimination or tissue defense mechanism decrease against them or both occur together, is called oxidative stress. In diabetes mellitus which is a glucose metabol...
Efficient energy transfer in cofacial boradiazaindacene (BODIPY) dyes built on a xanthane scaffold as a model for early steps in photosynthesis Dinç, Tarık; Akkaya, Engin Umut; Department of Biochemistry (2006) Energy transfer is a natural phenomena that bases of the photosynthesis. In photosynthesis, chlorophyls and auxlary absorbing dyes units absorb solar energy and transfer the energy to the reaction center where other chemical reactions occur. Studies showed that energy transfer depends strongly on distance and rigidy of the structure. Rigid structures that close in space transfer energy nearly %100 efficiency. Supramolecular chemistry tries to mimic nature and miniurize natural phenomence on to molecular sca...
THEORY OF POLARIZED FLUORESCENCE FROM MOLECULAR PAIRS - FORSTER TRANSFER AT LARGE ELECTRONIC COUPLING KNOX, RS; GULEN, D (Wiley, 1993-01-01) Polarization properties of the fluorescence from a pair of identical molecules coupled electronically are examined on the basis of a stochastic Liouville equation formalism developed in 1979 by Rahman, Knox and Kenkre. The time development of polarization is calculated for random ensembles of rigid molecule pairs under initial conditions that represent either selective excitation or broad-band coherent excitation. We hold that the applicability of the Forster mechanism is not limited to cases of weak coupli...
Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment. Huang, LY; Umanah, G; Hauser, M; Son, Çağdaş Devrim; Arshava, B; Naider, F; Becker, JM (American Chemical Society (ACS), 2008-05-20) Ste2p is the G protein-coupled receptor (GPCR) for the tridecapeptide pheromone cc factor of Saccharomyces cerevisiae. This receptor- pheromone pair has been used extensively as a paradigm for investigating GPCR structure and function. Expression in yeast harboring a cognate tRNA/aminoacyl-tRNA synthetase pair specifically evolved to incorporate p-benzoyl-L-phenylalanine (Bpa) in response to the amber codon allowed the biosynthesis of Bpa-substituted Ste2p in its native cell. We replaced natural amino acid ...
Benzaldehyde lyase from pseudomonas fluorescens biovar i mediated biotransformation for the synthesis of chiral alpha hydroxy ketones Hoşrik, Birsu Semra; Demir, Ayhan Sıtkı; Department of Biochemistry (2010) Optically active α-hydroxy ketones are important subunits of many biologically active compounds and indispensable synthons for asymmetric synthesis. Benzaldehyde Lyase from Pseudomonas fluorescens Biovar I is a novel ThDP-dependent enzyme that catalyzes the synthesis of benzoin type chiral α-hydroxy ketones starting from both benzaldehyde and racemic benzoin derivatives. Benzaldehyde Lyase is the first example of enzymes in the literature which leads to a chemical resolution of enantiomers of benzoin deriva...

Citation Formats

H. H. Kazan, C. Urfali-Mamatoglu, and U. Guenduez, “Resistance to anticancer drugs permanently alters electrophoretic mobility of cancer cell lines,” ELECTROPHORESIS, pp. 1201–1205, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66466.