Determination of Zinc, Copper, Iron, and Manganese in Different Regions of Lamb Brain

Date

2011-09-01

Author

Bakirdere, Sezgin
Kizilkan, Nese
YAMAN, MEHMET

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

119
views

0
downloads

The levels of zinc, copper, iron, and manganese were measured in the hippocampus, thalamus, gyrus cinguli, hypothalamus, and in the prefrontal, parietal, temporal, and occipital cortexes of lamb brain by flame atomic absorption spectrometry. Standard addition method was also applied to samples and it was observed that there was no matrix interference affecting the determination of elements interested. In the determination of Cu, a slotted tube atom trap was used to improve sensitivity. One-way analysis of variance was used for the statistical evaluation of the results for each element. Limit of detection results for Zn, Cu, Fe, and Mn were found to be 24, 4.5, 60, and 25 ng/mL, respectively. Region-specific differences were found for all elements interested. Mn concentration ranging from 0.29 +/- 0.04 to 0.61 +/- 0.04 mg/kg was found to be the lowest in all brain regions among the all elements interested.

Subject Keywords

Inorganic Chemistry, Clinical Biochemistry, Biochemistry, Endocrinology, Diabetes and Metabolism, Biochemistry, medical, General Medicine, Lamb brain, Zinc, Copper, Iron, Manganese

URI

https://hdl.handle.net/11511/66721

Journal

BIOLOGICAL TRACE ELEMENT RESEARCH

DOI

https://doi.org/10.1007/s12011-010-8804-0

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Determination of trace elements in marine plankton by inductively coupled plasma mass spectrometry (ICP-MS) Arslan, Z; Ertas, N; Tyson, JF; Uden, PC; Denoyer, ER (Springer Science and Business Media LLC, 2000-02-01) A method has been developed for the determination of 23 elements in marine plankton in which inductively coupled plasma (ICP) source mass spectrometry (MS) was used to quantify the elements in the solution after digestion in a mixture of hydrofluoric and nitric acids in sealed PTFE vessels in a microwave field. The procedure was validated by the analysis of a standard reference soil (SRM 2709 San Joaquin Soil) and a standard reference fresh water plankton (CRM 414). The method was applied to the analysis of...
Assessment of mineral density and atomic content of fracture callus by quantitative computerized tomography Korkusuz, Feza; Akın, Serhat; Akkuş, Ozan; KORKUSUZ, PETEK (Elsevier BV, 2000-01-01) The mineral density and atomic numbers of elements in the periosteal callus and the cortex area of a healing fracture were measured by quantitative computerized tomography (QCT) to obtain accurate information on the mineralization process in rabbit tibia. The mineral density of the periosteal callus was highest on day 15 and decreased gradually throughout the experiment. This was initially detected by QCT, but not with conventional radiography. An apparent decrease in cortical bone density on days 28 and 42...
KINETIC-PROPERTIES OF PURIFIED SHEEP LUNG MICROSOMAL NADH-CYTOCHROME B5 REDUCTASE Güray, Nülüfer Tülün (Elsevier BV, 1991-01-01) 1. Lung NADH-cytochrome b5 reductase was saturated with its artificial substrate, potassium ferricyanide at approximately 0.1 mM ferricyanide concentration, and the activity of the lung enzyme was inhibited by the higher concentrations of potassium ferricyanide. Ferricyanide at 0.5 and 1.0 mM inhibited the activity of the enzyme by about 20 and 61% respectively. The apparent K(m) value was calculated as 13.7-mu-M potassium ferricyanide and 4.3-mu-M NADH.
STUDIES ON METAL RESISTANCE SYSTEM IN KLUYVEROMYCES-MARXIANUS YAZGAN, A; Özcengiz, Gülay; ALAEDDINOGLU, NG (Springer Science and Business Media LLC, 1993-08-01) Through preliminary plate tests, Kluyveromyces marxianus was found to be much more resistant to toxic heavy metals compared to a CUP1R strain of Saccharomyces cerevisiae. Specific growth rate and maximum dry weights affected by increasing metal concentrations were determined to obtain precise patterns of resistance. Metal biosorption was also monitored during the course of growth in synthetic media containing respective metals at 0.5 mM final concentration. Although Zn- and Co-binding was negligible, as muc...
Isolation and immunological characterization of theta class glutathione-s-transferase gstt2-2 from bovine liver İşgör, Sultan Belgin; Çoruh, Nursen; Department of Biochemistry (2004) The glutathione-S-transferases (GSTs) (EC.2.5.1.18) are enzymes that participate in cellular detoxification of endogenous as well as foreign electrophilic compounds, function in the cellular detoxification systems and are evolved to protect cells against reactive oxygen metabolites by conjugating the reactive molecules to the nucleophile scavenging tripeptide glutathione (GSH, ?-glu-cys-gly). The GSTs are found in all eukaryotes and prokaryotic systems, in the cytoplasm, on the microsomes, and in the mitoch...

Citation Formats

S. Bakirdere, N. Kizilkan, and M. YAMAN, “Determination of Zinc, Copper, Iron, and Manganese in Different Regions of Lamb Brain,” BIOLOGICAL TRACE ELEMENT RESEARCH, pp. 492–499, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66721.