Treatment of silk production wastewaters by membrane processes for sericin recovery

Date

2008-12-01

Author

Capar, Goksen
Aygun, S. Seylan
Gecit, M. Rusen

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Sericin protein, although a valuable resource for many industries including cosmetics, pharmaceutical and biomedical, has been discarded as a waste in silk industry, causing environmental pollution. This paper describes determination of a membrane-based process for sericin recovery from cocoon cooking wastewaters (CCW) that will enable value-added utilization of waste sericin. The iso-electric point (pI) of sericin was found as 5-6, whose MW was distributed as 180-200, 70-80, 30-40 and 10-25 kDa. Prior to membrane filtration. sericin was separated from other impurities via centrifugation (CFG) followed by microfiltration (MF) in the pre-treatment stage, which also helped minimize post membrane fouling. Ultrafiltration (UF) and nanofiltration (NF) were adopted at a pH equal to pI of sericin. UF achieved partial recovery of sericin polypeptides at 37-60%, which was attributed to increased transmission of uncharged sericin polypeptides at their pI. On the other hand, NF achieved sericin recovery as high as 94-95%, containing all MW fractions. Severe flux decline was the major problem due to protein-membrane interactions and high sericin concentrations, where concentration polarization mainly had a dominant effect. Although flux declines were as high as 58-88% in UF and 70-75% in NF, flux recovery by at least 83% was achieved by chemical cleaning using NaOH and free chlorine.

Subject Keywords

Physical and Theoretical Chemistry, Filtration and Separation, General Materials Science, Biochemistry

URI

https://hdl.handle.net/11511/66965

Journal

JOURNAL OF MEMBRANE SCIENCE

DOI

https://doi.org/10.1016/j.memsci.2008.09.020

Collections

Department of Engineering Sciences, Article

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Citation Formats

G. Capar, S. S. Aygun, and M. R. Gecit, “Treatment of silk production wastewaters by membrane processes for sericin recovery,” JOURNAL OF MEMBRANE SCIENCE, pp. 920–931, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66965.