Experimental study of solute transport and extraction by a single root in soil

Kim, J
Chang, YS
Corapcioglu, MY
Drew, MC
The fate of C-14-2,4,6-trinitrotoluene ([U-C-14]TNT) in soil/plant systems was studied using onion (Allium cepa L.) plants with only a single root. It was found that the single roots grew exponentially and that the rate of water uptake of the onion plants increased exponentially, as well. The concentration of [U-C-14] in the roots at first increased and then appeared to reach a steady state, while the [U-C-14] concentration in the leaves was found to increase linearly with time. The [U-C-14] concentration in the rhizosphere increased gradually, while in the bulk soil it decreased slowly. The accumulation of [U-C-14] in the rhizosphere is likely to difference between movement into the rhizosphere (through advective mass flow of soil water by root uptake) and its uptake into the roots. The distribution of C-14 in the soil/plant system was found to be 60-85% in the soil solid phase, 7-11% in the soil liquid phase, <1% in the soil air phase, <1% in the root compartment, and <0.01% in the leaf compartment. The maximum RCF (root concentration factor) value for TNT and its derivates was found to be about 20, and the maximum TSCF (transpiration stream concentration factor) was 0.18. These values can be changed by a variety of factors in soil-plant systems.


Evaluation of photosynthetic performance of wheat cultivars exposed to boron toxicity by the JIP fluorescence test
Oz, M. T.; TURAN, ÖZLEM; Kayihan, C.; EYİDOĞAN, FÜSUN; EKMEKÇİ, YASEMİN; YÜCEL, MUSTAFA; Öktem, Hüseyin Avni (Institute of Experimental Botany, 2014-12-01)
The changes in growth and photosynthetic performance of two wheat (Triticum aestivum L.) cultivars (Bolal-2973 and Atay-85) differing in their sensitivity to boron (B) toxicity were investigated under toxic B conditions. Eight-day old seedlings were exposed to highly toxic B concentrations (5, 7.5, and 10 mM H3BO3) for 5 and 9 days. Fast chlorophyll a fluorescence kinetics was determined and analysed using JIP test. Growth parameters, tissue B contents, and membrane damage were measured at two stress durati...
Note: Pyrethroid resistance and possible involvement of glutathione S-transferases in Helicoverpa armigera from Turkey
Ugurlu, Sakine; Konus, Metin; Isgor, Belgin; İşcan, Mesude (Springer Science and Business Media LLC, 2007-01-01)
An introductory study was conducted to investigate the pyrethroid resistance of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) strains in Turkey, collected from cotton fields in the Adana and Antalya provinces, through two different synthetic pyrethroid insecticides: lambda-cyhalothrin and esfenvalerate. In addition, the roles of glutathione S-transferases (GSTs) in this resistance mechanism were analyzed. It was found that whereas resistance ratios for lambda-cyhalothrin (LD50 levels) were 3- and 9...
Genetic transformation of lentil ( Lens culinaris m. cv.Sultan.1) with a transcription factor regulator (MBF1c) and analysis of transgenic plants
Kamçı, Hamdi; Çelikkol Akçay, Ufuk; Kamçı, Hamdi; Department of Biotechnology (2011)
Agrobacterium mediated genetic transformation of lentil Sultan 1 cultivar with MBF1c and evaluation of transgenic plants was aimed. The study was initially based on optimized protocol with Agrobacterium tumefaciens KYRT1 strain and pTJK136 binary plasmid. Based on this protocol and transient marker gene expression in embryo apex, 15% stable transformation efficiency was aimed. However limited knowledge about pTJK136 and problem with curing KYRT1 leaded us to use Agrobacterium tumefaciens C58C1 strain and al...
Plasmalemma from the roots of cucumber: Isolation by two-phase partitioning and characterization
Memon, Abdul Razaque; Sommarin, Marianne; Kylin, Anders (Wiley, 1987-2)
Plasmalemma was isolated from the roots of 2‐week‐old cucumber plants (Cucumis sativus L. cv. Rhensk druv) by utilizing an aqueous polymer two‐phase system with 6.5%:6.5% (w/w) Dextran T500 and polyethylene glycol (PEG) 3350 at pH 7.8. The plasmalemma fraction comprised ca 6% of the membrane proteins contained in the microsomal fraction. The specific activity of the plasma membrane marker enzyme (K+, Mg2+‐ATPase) was 14‐ to 17‐times higher in the upper (PEG‐rich) than in the lower (Dextran‐rich) phase, and ...
Agrobacterium tumefaciens-mediated genetic transformation of a recalcitrant grain legume, lentil (Lens culinaris Medik)
Akcay, Ufuk Celikkol; Mahmoudian, M.; Kamci, H.; YÜCEL, MUSTAFA; Öktem, Hüseyin Avni (Springer Science and Business Media LLC, 2009-03-01)
A simple and reproducible Agrobacterium-mediated transformation protocol for a recalcitrant legume plant, lentil (Lens culinaris M.) is reported. Application of wounding treatments and efficiencies of three Agrobacterium tumefaciens strains, EHA105, C58C1, and KYRT1 were compared for T-DNA delivery into lentil cotyledonary node tissues. KYRT1 was found to be on average 2.8-fold more efficient than both EHA105 and C58C1 for producing transient beta-glucuronidase (GUS) gene (gus) expression on cotyledonary pe...
Citation Formats
J. Kim, Y. Chang, M. Corapcioglu, and M. Drew, “Experimental study of solute transport and extraction by a single root in soil,” PLANT AND SOIL, pp. 213–224, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67451.