Evaluation of photosynthetic performance of wheat cultivars exposed to boron toxicity by the JIP fluorescence test

Oz, M. T.
Kayihan, C.
Öktem, Hüseyin Avni
The changes in growth and photosynthetic performance of two wheat (Triticum aestivum L.) cultivars (Bolal-2973 and Atay-85) differing in their sensitivity to boron (B) toxicity were investigated under toxic B conditions. Eight-day old seedlings were exposed to highly toxic B concentrations (5, 7.5, and 10 mM H3BO3) for 5 and 9 days. Fast chlorophyll a fluorescence kinetics was determined and analysed using JIP test. Growth parameters, tissue B contents, and membrane damage were measured at two stress durations. The photochemical performance of PSII was hindered more in the sensitive cultivar (Atay-85) than that of the tolerant one (Bolal-2973) under B toxicity. The increase in the B concentration and stress duration caused membrane leakage in both cultivars. However, higher membrane damage was observed in Atay-85 compared to Bolal-2973. Additionally, significant reduction of growth parameters was observed in both cultivars at toxic B concentrations. The accumulation of B was higher in shoots than in roots of both cultivars. Nevertheless, Atay-85 translocated more B from roots to leaves compared to Bolal-2973. The advantages of certain JIP test parameters were demonstrated for evaluation of PSII activity in plants exposed to B stress. Evaluation of photosynthetic performance by JIP test as well as assessment of growth and tissue B content might be used to determine the effects of B toxicity in wheat. The results indicated lesser sensitivity to B toxicity in Bolal-2973 compared to Atay-85.


Effect of salt stress on antioxidant defense systems of sensitive and resistance cultivars of lentil(Lens culinaris M.)
Cicerali, Işın Nur; Yücel, Ayşe Meral; Department of Biotechnology (2004)
In this study, two lentil cultivars (Lens culinaris, Medik.) (ILL5582-salt tolerant and ILL590) were characterized and compared due to their NaCl susceptibility and antioxidant mechanism was examined under laboratory conditions. Physiological parameters such as wet-dry weight, root-shoot lengths, cell membrane stability, lipid peroxidation in terms of malondialdehyde (MDA), H2O2, proline contents were determined. The activities of antioxidant enzymes such as superoxide dismutase (SOD: EC, catalase...
Effect of drought and salt stresses on antioxidant defense system and physiology of lentil (lens culinaris m.) seedlings
Ercan, Oya; Öktem, Hüseyin Avni; Department of Biology (2008)
In this study, 14 days old lentil seedlings (Lens culinaris Medik cv. Sultan), which were subjected to 7 days of drought (20% PEG 6000), and salt (150 mM NaCl ) stress , were examined in a comparative manner for the effects of drought and salt stress treatments. In shoot and root tissues physiological parameters such as wet-dry weight, relative water content, root-shoot lengths, membrane electrolyte leakage, and lipid peroxidation in terms of malondialdehyde (MDA) were determined. H2O2 content, proline accu...
Expression profiling in response to ascochyta rabiei inoculations in chickpea
Avcıoğlu Dündar, Banu; Akkaya, Mahinur S.; Department of Biotechnology (2008)
In this study, it was aimed to identify chickpea (Cicer arietinum) genes or gene fragments expressed upon Ascochyta rabiei infection using a tolerant chickpea cultivar ILC195 and fungal isolates with varying level of pathogenicity. PCR amplification of resistance gene analogs (RGA) and disease related genes, and mRNA differential display reverse transcription (DDRT) were used to get these expressed gene fragments in chickpea. The constitutively or differentially expressed PCR product fragments were cloned a...
Optimization of regeneration and agrobacterium mediated transformation of sugar beet (Beta vulgaris L.)
Baloğlu, Mehmet Cengiz; Yücel, Ayşe Meral; Department of Biology (2005)
In this study, optimization of a transformation and regeneration system via indirect and direct organogenesis in cotyledon, hypocotyl, petiole, leaf and shoot base tissues of sugar beet (Beta vulgaris L. cv. ELK 345 and 1195) was investigated. Two different germination, three different callus induction and shoot induction medium was used for indirect organogenesis of sugar beet cultivar ELK 345. Except cotyledon, other explants (hypocotyl, petiole and leaf) produced callus. However no shoot development was ...
YUCEL, M; BURKE, JJ; NGUYEN, HT (Elsevier BV, 1992-04-01)
Two wheat (Triticum aestivum L.) cultivars, Mustang and Sturdy, have been shown to exhibit genetic diversity in acquired thermal tolerance following exposure to 37-degrees-C. The present study used chlorophyll fluorescence to investigate (1) the sensitivity of photosystem II (PS II) to 37-degrees-C treatments, and (2) the recovery of PS II variable fluorescence at 22-degrees-C following heat treatment. The purpose was to determine if the observed diversity in cellular thermotolerance was related to differen...
Citation Formats
M. T. Oz et al., “Evaluation of photosynthetic performance of wheat cultivars exposed to boron toxicity by the JIP fluorescence test,” PHOTOSYNTHETICA, pp. 555–563, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39852.