Kinetics model for growth of Pseudomonas putida F1 during benzene, toluene and phenol biodegradation

Date

2004-04-30

Author

Abuhamed, T
Bayraktar, E
Mehmetoglu, T
Mehmetoglu, U

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

238
views

0
downloads

The effect of adaptation of Pseudomonas putida F1 ATCC 700007 (Pp F1) to the biodegradation of benzene (B), toluene (T) and phenol (P) was studied. The adaptation of microorganism to BTP decreased the biodegradation time from 24 to 6 It for benzene (90 mg/1) and toluene (90 mg/1), and from 90 to 18 It for phenol (50 mg/1). Andrews kinetics model for single substrate was solved to obtain maximum specific growth rates, half saturation and substrate inhibition constant. Cell growth using toluene (mu(max.T) = 0.61) and benzene (mu(max.B) = 0.62) as carbon sources were better and faster than the growth in phenol (mu(max.P) = 0.051). For the substrate mixtures, a sum kinetics model was used and the interaction parameters were determined. These models provided an excellent prediction of the growth kinetics and the interactions between these substrates. Toluene inhibited the utilization of benzene (I-T, B = 5.16) much more than benzene inhibits the utilization of toluene (I-B.T = 0.49). Benzene (I-B.P = 0.27) and toluene (I-T.P= 0.14) enhance the biodegradation of phenol, and phenol inhibits the biodegradation of benzene (I-P.B = 1.08) and toluene (I-P.T = 1.03).

Subject Keywords

Applied Microbiology and Biotechnology, Biochemistry, Bioengineering

URI

https://hdl.handle.net/11511/67475

Journal

PROCESS BIOCHEMISTRY

DOI

https://doi.org/10.1016/s0032-9592(03)00210-3

Collections

Department of Petroleum and Natural Gas Engineering, Article

Suggestions

OpenMETU
Core

Substrate interactions during the biodegradation of benzene, toluene and phenol mixtures Abu Hamed, T; Bayraktar, E; Mehmetoglu, T; Mehmetoglu, U (Elsevier BV, 2003-09-30) Benzene, toluene and phenol were degraded completely at high initial concentrations by Pseudomonas putida F I ATCC 700007. Two hundred and fifty milligram per litre benzene, 225 mg/l toluene and 200 mg/l phenol were degraded individually in 19, 14 and 3 5 h, respectively. The biodegradation times increased on increasing the substrate concentration. The maximum biodegradation rates were 149 mg benzene/g dry cell h for 60 mg/l benzene, 44 mg toluene/g dry cell h for 110 mg/l toluene and 102 mg phenol/g dry ce...
Kinetics of riboflavin production by Brewers' yeasts Tamer, I.M.; Özilgen , Mustafa; Ungan, Suat (Elsevier BV, 1988-12) The kinetics of riboflavin production by Saccharomyces cerevisiae and Saccharomyces carlsbergensis in synthetic media and wort were studied. The results indicated that riboflavin was produced by growing cells only. Riboflavin production rate was proportional to growth rate of the yeasts in the exponential phase. Riboflavin was depleted in the stationary phase. The depletion rate was expressed with a first-order kinetic expression in yeast concentration. The kinetics of substrate utilization and ethanol prod...
Mechanistics of nickel sorption by activated sludge Arican, B; Gokcay, CF; Yetiş, Ülkü (Elsevier BV, 2002-06-01) Biomass from activated sludge reactors operating at different dilution rates (0.09, 0,16, and 0.24/h) was used to examine the effect of sludge age on Ni2+ removal characteristics, running batch adsorption tests. The kinetic studies have revealed that sludge grown at all dilution rates, exhibits both active and passive uptake of Ni2+. Although percentages of active and passive uptake changed with the age of activated sludge biomass, passive uptake was the dominant mechanism in Ni2+ sorption. The data obtaine...
Kinetic analyses of the effects of temperature and light intensity on growth, hydrogenm production and organic acid utilization by rhodobacter capsulatus Sevinç, Pelin; Gündüz, Ufuk; Department of Biotechnology (2010) Effects of temperature and light intensity on photofermentative hydrogen production by Rhodobacter capsulatus DSM1710 by use of acetic and lactic acids as substrates were studied. Experiments were conducted at 20, 30 and 38oC incubator temperatures under light intensities in the 1500 – 7000 lux range. pH of the medium and quantity of hydrogen forming together with quantity of biomass, and concentrations of acetic, lactic, formic, butyric and propionic acids in the medium were determined periodically. Growth...
Preparation of cross-linked tyrosinase aggregates Aytar, Burcu Selin; Bakir, Ufuk (Elsevier BV, 2008-02-01) Tyrosinase from mushroom was immobilized as a cross-linked enzyme aggregate (CLEA) via precipitation with ammonium sulfate and cross-linking with glutaraldehyde. The effects of precipitation and cross-linking on CLEA activity were investigated and the immobilized tyrosinase was characterized. Sixty percent ammonium sulfate saturation and 2% glutaraldehyde were used; a 3-h cross-linking reaction at room temperature, at pH 7.0 was performed; particle sizes of the aggregates were reduced; consequently, 100% ac...

Citation Formats

T. Abuhamed, E. Bayraktar, T. Mehmetoglu, and U. Mehmetoglu, “Kinetics model for growth of Pseudomonas putida F1 during benzene, toluene and phenol biodegradation,” PROCESS BIOCHEMISTRY, pp. 983–988, 2004, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67475.