Kinetics model for growth of Pseudomonas putida F1 during benzene, toluene and phenol biodegradation

Abuhamed, T
Bayraktar, E
Mehmetoglu, T
Mehmetoglu, U
The effect of adaptation of Pseudomonas putida F1 ATCC 700007 (Pp F1) to the biodegradation of benzene (B), toluene (T) and phenol (P) was studied. The adaptation of microorganism to BTP decreased the biodegradation time from 24 to 6 It for benzene (90 mg/1) and toluene (90 mg/1), and from 90 to 18 It for phenol (50 mg/1). Andrews kinetics model for single substrate was solved to obtain maximum specific growth rates, half saturation and substrate inhibition constant. Cell growth using toluene (mu(max.T) = 0.61) and benzene (mu(max.B) = 0.62) as carbon sources were better and faster than the growth in phenol (mu(max.P) = 0.051). For the substrate mixtures, a sum kinetics model was used and the interaction parameters were determined. These models provided an excellent prediction of the growth kinetics and the interactions between these substrates. Toluene inhibited the utilization of benzene (I-T, B = 5.16) much more than benzene inhibits the utilization of toluene (I-B.T = 0.49). Benzene (I-B.P = 0.27) and toluene (I-T.P= 0.14) enhance the biodegradation of phenol, and phenol inhibits the biodegradation of benzene (I-P.B = 1.08) and toluene (I-P.T = 1.03).


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Benzene, toluene and phenol were degraded completely at high initial concentrations by Pseudomonas putida F I ATCC 700007. Two hundred and fifty milligram per litre benzene, 225 mg/l toluene and 200 mg/l phenol were degraded individually in 19, 14 and 3 5 h, respectively. The biodegradation times increased on increasing the substrate concentration. The maximum biodegradation rates were 149 mg benzene/g dry cell h for 60 mg/l benzene, 44 mg toluene/g dry cell h for 110 mg/l toluene and 102 mg phenol/g dry ce...
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Citation Formats
T. Abuhamed, E. Bayraktar, T. Mehmetoglu, and U. Mehmetoglu, “Kinetics model for growth of Pseudomonas putida F1 during benzene, toluene and phenol biodegradation,” PROCESS BIOCHEMISTRY, pp. 983–988, 2004, Accessed: 00, 2020. [Online]. Available: