Determination of esterase enzyme polymorphism in house fly (Musca domestica L.) populations in Turkey

Taskin, Belgin Gocmen
Kence, Meral
The objective of this study was to determine esterase enzyme polymorphism in 16 natural Musca domestica L. populations from the Aegean and Mediterranean regions of Turkey by using the native polyacrylamide gel electrophoresis (PAGE) technique. The high level of genetic variation among populations and species make esterase enzymes an important tool for analyzing genetic differentiation and evolutionary relationships in insects. In the present study, 22 a-esterase bands were detected by using a-naphthylacetate as a substrate. The frequencies of these bands were variable within and among populations. Neither regional nor population specific esterase bands were detected. The origin of speciation of M. domestica is unknown, but the southern Palearctic region, particularly the Middle East, has been suggested as the likely candidate location. Studies on genetic diversity in M. domestica are important for providing knowledge that will better enable us to understand the origin of this species. This preliminary study is important because it is the first in the literature to illustrate the esterase isozymes and their frequencies for this part of the world. For a better evaluation and understanding of the ongoing situation of M. domestica populations in Turkey as a Palearctic country, however, further research is needed to screen not only populations from these 2 regions but also from other regions for many other enzyme and DNA loci.


The impact of over-exploitation on the genetic structure of Turkish red pine (Pinus brutia Ten.) populations determined by RAPD markers
Lise, Yildiray; Kaya, Zeki; Isik, Fikret; Sabuncu, Rumi; Kandemir, Irfan; Önde, Sertaç (2007-01-01)
To determine the possible impact of over-exploitation on the genetic structure of Turkish red pine (Pinus brutia Ten.) populations, three natural and three over-exploited (human degraded) populations of the species in the Mediterranean region of Turkey were investigated with Randomly Amplified Polymorphic DNA (RAPD). With the 80 RAPT) primers tested, 12 of them yielded 137 polymorphic RAPD fragments. Four of the studied populations maintained unique fragments. The mean proportion of polymorphic fragments fo...
Kaya, Zeki (1994-01-01)
To determine the genetic structure of marginally located populations of Pinus nigra var pallasiana, seedlings of open pollinated families from 7 populations were raised in Kizilcahamam nursery near Ankara for 2 years. Seed weight (SW) and cone weight (CW) for families, number of cotyledon per seedling (GOT), timing of bud set in 1990 (BS90) and in 1991 (BS91), bud burst timing of seedlings in 1991 (BB91), height growth in 1991 (HT90) and final height growth in 1991 (HT91) and final diameter growth of seedli...
Assessment of pollution in the West Black Sea Coast of Turkey using biomarker responses in fish
BOZCAARMUTLU, AZRA; Sapmaz, Canan; Aygun, Zuleyha; Arinc, Emel (Elsevier BV, 2009-05-01)
Aim of this study was to determine the extent of pollution in the West Black Sea Coast of Turkey by measuring CYP1A associated EROD activity, phase II enzyme, glutathione S-transferase and antioxidant enzymes, catalase and glutathione reductase activities and immunochemical detection of CYP1A protein level in the liver of mullet. The fish samples were caught from six locations having a varying degree of pollution in the West Black Sea Region of Turkey in August 2005, 2006 and 2007. Mullets caught from Zongu...
ELHALLAQ, YH; GOKMEN, IG; ARAS, NK; Gökmen, Ali (Royal Society of Chemistry (RSC), 1992-03-01)
A post-irradiation radiochemical separation technique was tested for the determination of selenium levels in diet samples, collected by using a duplicate portion technique, from both rural and urban population groups in Turkey. The technique involved sample irradiation, acid digestion, selective distillation, precipitation and filtration steps. During the separations it was possible to determine the yield of each sample using a stable selenium carrier. An average chemical yield of 71 +/- 3% was obtained ...
The pattern of genetic variation in shoot growth of Pinus brutia Ten. populations sampled from the Toros mountains in Turkey
Kaya, Zeki (1997-01-01)
The pattern of genetic variation in shoot growth of Turkish red pine (Pinus brutia TEN.) was studied in 180 open-pollinated families from a south to north transect in southern Turkey. Seedlings from 1 coastal, 1 inland and 2 centrally located populations (45 open-pollinated families each) were grown for 2 growing seasons in a forest nursery located near Antalya. The study revealed that both populations and families within populations varied significantly in all seedling traits except for free growth in the ...
Citation Formats
V. TAŞKIN, B. G. Taskin, K. KÜÇÜKAKYÜZ, and M. Kence, “Determination of esterase enzyme polymorphism in house fly (Musca domestica L.) populations in Turkey,” TURKISH JOURNAL OF ZOOLOGY, pp. 869–877, 2011, Accessed: 00, 2020. [Online]. Available: