Investigating physicochemical properties of wetglycated soy proteins

Download

12625647.pdf

Date

2020-8

Author

Zia, Muhammed Bin

Metadata

Show full item record

Item Usage Stats

441
views

174
downloads

On a lab-scale, glycation is generally a lengthy process which if replicated on an industrial scale, would be costly. In this study, a simplified method for glycating soy protein isolate has been developed. Soy proteins were glycated with allulose, fructose and glucose in the presence of water. The protein-water ratio was changed from ½ to 1 (w/w) and glycation was conducted at 100°C and 130°C for 15 minutes, and samples were also dried at 50°C, 55% relative humidity for 1 day. The physicochemical properties of these proteins were analysed including free amino groups by OPA method, protein solubility by Lowry method, browning index, reducing sugar concentration using HPLC, structural changes via Fourier Transform Infrared (FT-IR) spectroscopy and hydration behavior using Time Domain Nuclear Magnetic Resonance (TD-NMR) relaxometry through T2 relaxation time measurements. The results showed that proteins could be glycated under these simple conditions and with minimal control. It was found that high temperatures are not favorable for glycation and lower temperatures could be preffered. At high temperatures, proteins become more denatured and less soluble as shown through the Lowry method for protein solubility and TD-NMR results. Higher browning was seen at higher temperature, especially when allulose and fructose were used for vi glycation. Proteins glycated with glucose were observed to be lighter in color. The highest glycation was obtained with glucose while allulose and fructose showed the lowest reactivity under the proposed conditions. Proteins with low solubility had longer T2 relaxation times, indicating the presence of more free water and weaker water-binding capability.

Subject Keywords

Glycation, Soy protein isolate, FT-IR, TD-NMR, Glikasyon, Soya protein izolatı

URI

https://hdl.handle.net/11511/69011

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Physicochemical properties of wet-glycated soy proteins Zia, Muhammad Bin; Namlı, Serap; Öztop, Halil Mecit (2021-05-01) On a lab-scale, glycation is generally a lengthy process that would be costly if replicated on an industrial scale. In this study, a simplified method for glycating soy protein isolate has been developed. Soy proteins were glycated with allulose, fructose, and glucose in the presence of water. Free amino group content, protein solubility, browning index, and reducing sugar concentration were measured for the glycated proteins. Fourier Transform Infrared (FT-IR) spectroscopy was used to examine structural ch...
INVESTIGATION OF DAMAGE IN GFRP TAPERED COMPOSITE LAMINATES Hosseinpour Dashatan, Saeid; Arıkan, Mehmet Ali Sahir; Parnas, Kemal Levend; Department of Mechanical Engineering (2021-8-13) In some applications of composite laminates, thickness variation is necessary to fulfill specific design requirements. One way of accomplishing the intentional thickness variation in composite structures is terminating some plies within a layup. Introducing ply drop-offs causes geometrical and material discontinuities, which lead to high localized stresses around drop-off locations. Despite all the progress made in damage analyses of tapered composite laminates, estimating their load-carrying capacity and s...
Study of sol-gel processing for fabrication of low density alumina microspheres Dilsiz, N; Akovali, G (2002-07-01) A sol-gel process for producing microspheres of low density alumina has been developed. A Dispal alumina sol and aluminum monohydrate were used as starting materials. Microspheres with spherical shape and controllable size have been produced by using a drop generation technique, The technique consists of producing a continuous stream Of uniform droplets of alumina sol and then converting the drops into a rigid form by exposing them to an ammonium hydroxide gelation medium. By using this technique. alumina s...
Investigation of Changes in mRNA Levels of Sporulation Related Genes in OGU1 Strain in Comparison to Standard Strain PY79 of Bacillus subtilis Özcengiz, Gülay(2018-12-31) Bacillus subtilis çok sayıda ve çeşitte ikincil metabolit üretmesiyle bilinmektedir. Bunlardan biri olan basilisin, L-alanin ve L-antikapsinden ribozomal olmayan bir şeklide üretilir. Basilisin antifungal ve çeşitli bakterilere karşı etkilidir ve L-antikapsinin salınması ile hücre duvarı üretiminde görevli glukozamin sentetaz enziminin inhibisyonunu sağlar. Grubumuzca oluşturulan basilisin üretemeyen mutant OGU1 suş ile gerçekleştirilen karşılaştırmalı proteom analizleri sonucunda, biyofilm, sporülasyon, hü...
Comparison of Fine Grained and Coarse Grained Parallel Models in Particle Swarm Optimization Algorithm Baştürk, Alper; Akay, Rüştü; Kalınlı, Adem (2011-11-23) Many optimization problems are generally complex and required to be solved in parallel architectures due to theircomputational costs. The main issue about the parallelism is that the parallel architectures may affect the performancebecause the original models are constructed based upon the sequential architectures. Therefore, the parallelizationapproaches should consider the efficiency in addition to reducing computational cost. The objective of this paper is two-fold.First goal is presenting a parallelizat...

Citation Formats

M. B. Zia, “Investigating physicochemical properties of wetglycated soy proteins,” M.S. - Master of Science, Middle East Technical University, 2020.