Improvement of pea proteins’ properties by microwave glycation

Download
2020-9
Ertuğrul, Ülkü
The tendency to include plant proteins in the diet has increased significantly as consumers' preference for animal proteins decline. Sustainability goals, low manufacturing cost, and high nutritional value are also triggering this demand. For this reason, pea protein as a form of isolate and concentrate is often used in protein enriched diets due to its nutritive properties. Previously, improvement in its functional properties has been studied from many perspectives. Glycation, which is known as the initial stage of the Maillard reaction can be considered as a modification strategy for improving the functional properties. In this study, microwave was used for glycating pea protein concentrate. The objective of the study was to investigate the extent of microwave glycation between pea protein concentrate (PPC) and reducing sugars of dextrose, fructose, and the rare sugar, Allulose, by determining the soluble proteins, free amino groups, remaining reducing sugars and advanced glycation end products (AGEs). Also, the effect of two different pH values (7 and 10) and two different pea protein to sugar ratios (2:1.25 and 9:1.25) were observed. Solubility and hydration behavior of PPC were investigated vi afterwards. Lowry method proved that microwave glycation enhanced the solubility of dextrose samples by 50% compared to control samples. Also, the alkaline environment improved PPC solubility. Besides that, microwave glycation improved the hydration of PPC more when it is compared to water bath glycation. To conclude, the study showed that microwave glycation could be used as an alternative modification method since it improved the water-pea protein concentrate interactions more than water bath glycation

Suggestions

Pea protein properties are altered following glycation by microwave heating
Ertuğrul, Ülkü; Namlı, Serap; Taş, Ozan; Şümnü, Servet Gülüm; Öztop, Halil Mecit (2021-10-01)
Pea protein is often used in protein enriched diets due to its nutritional properties. Modifying its properties has been studied from many perspectives. Glycation, a non-enzymatic glycosylation reaction of proteins, is one of the modification strategies used to alter its properties like solubility and hydration ability. This study aims to investigate the extent and effect of glycation by MW heating with reducing sugars (glucose, fructose and allulose) on pea protein concentrate (PPC) by determining protein ...
Physical and chemical characterization of sesame seed protein
Köysüren, Begüm; Öztop, Halil Mecit; Bekir Gökçen , Mazı; Department of Food Engineering (2020-9)
Plant-based food proteins have become very popular and valuable due to increase trend of following a vegan diet and preferring to consume less meat in terms of sustainability reasons. The most popular plant based proteins are soya bean, quinoa, lentil, peanut and chia seed proteins. Sesame seed also has a potential to be an alternative protein source. However, it is a known fact that there is no extended study about sesame seed proteins in the literature although sesame seed contains significant amoun...
Current applications of exopolysaccharides from lactic acid bacteria in the development of food active edible packaging
Moradi, Mehran; Guimarães, Jonas T; Şahin, Serpil (Elsevier BV, 2021-08-01)
The lactic acid bacteria (LAB) are usually recognized as safe for consumption and comprise several genera with different technological and health-promoting potential for food applications, including probiotic characteristics. Their exopolysaccharides (EPS) have interesting film-forming properties and may be used to produce edible packaging, as a structural enhancer, a bioactive agent or probiotic carrier in edible films and coatings. In these days, there is a specific demand for food products with reduced a...
Improvement of holdase activity of a small heat shock protein from extremely acidophilic archaea
Kaçer, Yağmur; Kocabıyık, Semra; Department of Biochemistry (2019)
In this study, a small heat shock protein gene (po-sHSP20) of archaeon Picrophilus oshimae which is the most thermoacidophilic organism known, was cloned and overexpressed in E.coli for the first time. The recombinant po-sHSP20 protein that was resistant to high temperature was purified to homogeneity by affinity chromatography. Oligomeric structure analysis of the po-sHSP20 by Size Exclusion Chromatography showed that it exists mostly as 12- and 18-mer oligomers with some dimeric/monomeric units under phys...
Induced CYP1A mRNA, protein and catalytic activity in the liver of feral fish, leaping mullet, Liza saliens
Arinc, E; Kocabıyık, Semra; Su, E (2001-02-01)
In this study, we examined whether levels of P4501A mRNA expression were naturally induced in feral fish, Liza saliens, and whether CYP1A protein levels and associated enzyme activity, EROD, were also increased. Induction of mRNA was measured using a nucleic acid hybridization technique. For the hybridization studies, a new 33-mer oligonucleotide probe 5 ' -dCTC ATC CAG CTT CCT GTC CTC GCA GTG ATC AAT-3 ' was designed, which corresponded to the totally conserved amino acid motif of CYP1A protein from positi...
Citation Formats
Ü. Ertuğrul, “Improvement of pea proteins’ properties by microwave glycation,” M.S. - Master of Science, Middle East Technical University, 2020.