Exploring Marine Microbial Community Structure Using 16S rRNA Amplicon Sequencing: First results to understand their role in the oligotrophic marine ecosystem

2017-07-13
Küüçükavşar, Selin
Karahan, Arzu
Arkın, Sinan
Biogeochemical cycle, biotechnology or basic researches done by standard methods (culturing) provide biased and limited information. Next Generation Sequencing (NGS) platforms are the best tool that uses culture-independent approaches and thus provides data related to the microbial genetic diversity, while the organic matter produced by marine plants (photosynthesis) sinks to the bottom of the sea, some are exposed to decomposition by bacteria that produce the inorganic nutrients necessary for marine plants. This recycling/decomposition performed by bacteria makes them a central component of the marine biogeochemical cycles, which in turn signifies their importance in some of the most important environmental problems facing the world, including climate change and eutrophication. Despite the many important roles they play, bacteria are one of the least known components of the marine biogeochemical cycles and their representation in the marine biogeochemical models is highly limited. Here we will present the first culture independent 16S rRNA amplicon sequencing study results in the northeastern 4thEcology and Evolutionary Biology Symposium, Turkey, 201721Mediterranean Sea. Seawater sample collected from the surface in the 200 meters’ station of the Erdemli Time Series stations and sequenced by next generation sequencing platform to explore community composition and to understand their functional diversity.The number of OTUs assigned is 143 and 96% of it was classified as Bacteria, 1% as Archaea and 3% unclassified. It is the first data to develop a gene-centric model to determine the rates of the reactions that the bacteria perform.
Citation Formats
S. Küüçükavşar, A. Karahan, and S. Arkın, “Exploring Marine Microbial Community Structure Using 16S rRNA Amplicon Sequencing: First results to understand their role in the oligotrophic marine ecosystem,” İTÜ Ayazağa Campus, Istanbul, Turkey, 2017, p. 20, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/71959.