Protein-Protein Interactions in Live Cells: Reinventing the Wheel

2018-12-13
G protein-coupled receptors (GPCRs) are membrane proteins that mediate physiologicalresponse to a diverse array of stimuli. In humans, they mediate the action of hundreds ofpeptide hormones, sensory stimuli, odorants, neurotransmitters, and chemokines. GPCRs alsoare targets for ~40% of all currently marketed pharmaceuticals. These receptors traditionallybeen thought to act as monomeric units. However, recent evidence suggests that GPCRs mayform dimers as part of their normal trafficking and function. While the formation of GPCRdimers/oligomers have been reported to play important roles in regulating receptorexpression, ligand binding, and second messenger activation, less is known about how GPCRdimers interact with other proteins such as G-proteins and Arrestin.We are interested in studiying the interactions between GPCRs and effect of this dimerizationon G-protein dimerization. Our group also focus on the mechanisms of receptor-arrestinbinding in live cells using Föster resonance energy transfer (FRET) and bimolecularfluorescence complementation (BiFC) assays. We designed and developed tagged receptors,G-proteins and Arrestin proteins using Green florescent protein variants that can be used inimaging studies. These constructs are suitable for testing drug candidates and/or analyzeprotein-protein interfaces for GPCRs or G-protein dimers.
6th International Drug Design Congress

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Citation Formats
Ç. D. Son, “Protein-Protein Interactions in Live Cells: Reinventing the Wheel,” presented at the 6th International Drug Design Congress, İstanbul, Turkey, 2018, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/73745.